Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1007 and GFF1008 are separated by 18 nucleotides GFF1008 and GFF1009 are separated by 53 nucleotides GFF1009 and GFF1010 are separated by 80 nucleotides
GFF1007 - Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22), at 326,586 to 327,110
GFF1007
GFF1008 - Glyoxalase family protein, at 327,129 to 327,581
GFF1008
GFF1009 - Transcriptional regulator, AraC family, at 327,635 to 328,501
GFF1009
GFF1010 - hypothetical protein, at 328,582 to 330,915
GFF1010
Position (kb) 327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 326.784 kb on + strand, within GFF1007 at 326.784 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.940 kb on + strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 326.941 kb on - strand, within GFF1007 at 327.009 kb on + strand, within GFF1007 at 327.009 kb on + strand, within GFF1007 at 327.009 kb on + strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.010 kb on - strand, within GFF1007 at 327.063 kb on + strand at 327.063 kb on + strand at 327.063 kb on + strand at 327.063 kb on + strand at 327.063 kb on + strand at 327.064 kb on - strand at 327.064 kb on - strand at 327.064 kb on - strand at 327.064 kb on - strand at 327.064 kb on - strand at 327.064 kb on - strand at 327.161 kb on - strand at 327.161 kb on - strand at 327.161 kb on - strand at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.259 kb on + strand, within GFF1008 at 327.260 kb on - strand, within GFF1008 at 327.260 kb on - strand, within GFF1008 at 327.260 kb on - strand, within GFF1008 at 327.260 kb on - strand, within GFF1008 at 327.389 kb on + strand, within GFF1008 at 327.390 kb on - strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.517 kb on + strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.518 kb on - strand, within GFF1008 at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.556 kb on + strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.557 kb on - strand at 327.636 kb on + strand at 327.636 kb on + strand at 327.636 kb on + strand at 327.636 kb on + strand at 327.637 kb on - strand at 327.637 kb on - strand at 327.637 kb on - strand at 327.637 kb on - strand at 327.774 kb on + strand, within GFF1009 at 327.774 kb on + strand, within GFF1009 at 327.774 kb on + strand, within GFF1009 at 327.774 kb on + strand, within GFF1009 at 327.775 kb on - strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.809 kb on + strand, within GFF1009 at 327.810 kb on - strand, within GFF1009 at 327.810 kb on - strand, within GFF1009 at 327.810 kb on - strand, within GFF1009 at 327.810 kb on - strand, within GFF1009 at 327.861 kb on + strand, within GFF1009 at 327.861 kb on + strand, within GFF1009 at 327.861 kb on + strand, within GFF1009 at 327.861 kb on + strand, within GFF1009 at 327.861 kb on + strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.862 kb on - strand, within GFF1009 at 327.921 kb on + strand, within GFF1009 at 327.921 kb on + strand, within GFF1009 at 327.922 kb on - strand, within GFF1009 at 328.086 kb on + strand, within GFF1009 at 328.087 kb on - strand, within GFF1009 at 328.153 kb on + strand, within GFF1009 at 328.154 kb on - strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.260 kb on + strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.261 kb on - strand, within GFF1009 at 328.610 kb on + strand at 328.610 kb on + strand at 328.610 kb on + strand at 328.610 kb on + strand at 328.610 kb on + strand at 328.610 kb on + strand at 328.611 kb on - strand at 328.611 kb on - strand at 328.611 kb on - strand at 328.611 kb on - strand at 328.611 kb on - strand at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.940 kb on + strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 328.941 kb on - strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.054 kb on + strand, within GFF1010 at 329.055 kb on - strand, within GFF1010 at 329.055 kb on - strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.258 kb on + strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.259 kb on - strand, within GFF1010 at 329.285 kb on + strand, within GFF1010 at 329.286 kb on - strand, within GFF1010 at 329.286 kb on - strand, within GFF1010
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 326,784 + GFF1007 0.38 -2.0 326,784 + GFF1007 0.38 -0.4 326,940 + GFF1007 0.67 +1.4 326,940 + GFF1007 0.67 -0.1 326,940 + GFF1007 0.67 -1.1 326,940 + GFF1007 0.67 -0.6 326,940 + GFF1007 0.67 -0.6 326,940 + GFF1007 0.67 +0.6 326,940 + GFF1007 0.67 -0.8 326,940 + GFF1007 0.67 -0.1 326,941 - GFF1007 0.68 -0.8 326,941 - GFF1007 0.68 -0.2 326,941 - GFF1007 0.68 -0.5 326,941 - GFF1007 0.68 -0.5 326,941 - GFF1007 0.68 -0.1 326,941 - GFF1007 0.68 -0.0 326,941 - GFF1007 0.68 +0.2 326,941 - GFF1007 0.68 +0.6 326,941 - GFF1007 0.68 +0.2 327,009 + GFF1007 0.81 +1.1 327,009 + GFF1007 0.81 +1.6 327,009 + GFF1007 0.81 -0.7 327,010 - GFF1007 0.81 +0.5 327,010 - GFF1007 0.81 +0.8 327,010 - GFF1007 0.81 +0.1 327,010 - GFF1007 0.81 -0.2 327,010 - GFF1007 0.81 -0.8 327,010 - GFF1007 0.81 +0.4 327,063 + +0.1 327,063 + +0.4 327,063 + +0.6 327,063 + +0.9 327,063 + -0.3 327,064 - -0.6 327,064 - -0.3 327,064 - -1.2 327,064 - -1.1 327,064 - +1.4 327,064 - -1.1 327,161 - -0.2 327,161 - +0.1 327,161 - +0.6 327,259 + GFF1008 0.29 -0.3 327,259 + GFF1008 0.29 -0.1 327,259 + GFF1008 0.29 -0.8 327,259 + GFF1008 0.29 -0.8 327,259 + GFF1008 0.29 +0.4 327,259 + GFF1008 0.29 +0.6 327,259 + GFF1008 0.29 +0.0 327,260 - GFF1008 0.29 -0.9 327,260 - GFF1008 0.29 -0.2 327,260 - GFF1008 0.29 -0.7 327,260 - GFF1008 0.29 -0.7 327,389 + GFF1008 0.57 -0.3 327,390 - GFF1008 0.58 +0.2 327,517 + GFF1008 0.86 -1.1 327,517 + GFF1008 0.86 -0.7 327,517 + GFF1008 0.86 -0.1 327,517 + GFF1008 0.86 -0.2 327,517 + GFF1008 0.86 -1.2 327,517 + GFF1008 0.86 +0.6 327,517 + GFF1008 0.86 -1.0 327,517 + GFF1008 0.86 -0.7 327,517 + GFF1008 0.86 +0.3 327,517 + GFF1008 0.86 +0.8 327,517 + GFF1008 0.86 -0.2 327,518 - GFF1008 0.86 -0.4 327,518 - GFF1008 0.86 +0.3 327,518 - GFF1008 0.86 +1.4 327,518 - GFF1008 0.86 +0.2 327,518 - GFF1008 0.86 -0.3 327,518 - GFF1008 0.86 +1.5 327,518 - GFF1008 0.86 +0.1 327,518 - GFF1008 0.86 +0.4 327,518 - GFF1008 0.86 +2.9 327,518 - GFF1008 0.86 -1.1 327,518 - GFF1008 0.86 -1.6 327,556 + +0.0 327,556 + -0.7 327,556 + -0.7 327,556 + -0.5 327,556 + -1.0 327,556 + -1.1 327,556 + -0.3 327,556 + -0.2 327,556 + +0.5 327,556 + +0.2 327,556 + +3.7 327,556 + +0.2 327,557 - -0.3 327,557 - -0.8 327,557 - +0.7 327,557 - -0.1 327,557 - -0.0 327,557 - -0.2 327,557 - -0.6 327,557 - +0.4 327,557 - -0.3 327,557 - +0.1 327,557 - +0.6 327,636 + -1.2 327,636 + -0.3 327,636 + +0.6 327,636 + -0.5 327,637 - +1.8 327,637 - +0.3 327,637 - -0.0 327,637 - -0.2 327,774 + GFF1009 0.16 +0.0 327,774 + GFF1009 0.16 -0.3 327,774 + GFF1009 0.16 +0.7 327,774 + GFF1009 0.16 -0.2 327,775 - GFF1009 0.16 -0.2 327,809 + GFF1009 0.20 -0.4 327,809 + GFF1009 0.20 -1.2 327,809 + GFF1009 0.20 +0.0 327,809 + GFF1009 0.20 +0.4 327,809 + GFF1009 0.20 -0.4 327,809 + GFF1009 0.20 -0.2 327,810 - GFF1009 0.20 +0.4 327,810 - GFF1009 0.20 -0.4 327,810 - GFF1009 0.20 -0.5 327,810 - GFF1009 0.20 -0.3 327,861 + GFF1009 0.26 -1.0 327,861 + GFF1009 0.26 -0.3 327,861 + GFF1009 0.26 -0.6 327,861 + GFF1009 0.26 -1.6 327,861 + GFF1009 0.26 -1.4 327,862 - GFF1009 0.26 -0.4 327,862 - GFF1009 0.26 -0.2 327,862 - GFF1009 0.26 -0.4 327,862 - GFF1009 0.26 +1.2 327,862 - GFF1009 0.26 -0.2 327,862 - GFF1009 0.26 +0.1 327,921 + GFF1009 0.33 -0.4 327,921 + GFF1009 0.33 +0.0 327,922 - GFF1009 0.33 -0.0 328,086 + GFF1009 0.52 +0.3 328,087 - GFF1009 0.52 -0.3 328,153 + GFF1009 0.60 -0.9 328,154 - GFF1009 0.60 +0.1 328,260 + GFF1009 0.72 -0.9 328,260 + GFF1009 0.72 -0.4 328,260 + GFF1009 0.72 -0.8 328,260 + GFF1009 0.72 -0.4 328,260 + GFF1009 0.72 +0.0 328,260 + GFF1009 0.72 -3.0 328,260 + GFF1009 0.72 -0.3 328,260 + GFF1009 0.72 +0.4 328,260 + GFF1009 0.72 -0.4 328,260 + GFF1009 0.72 -0.0 328,260 + GFF1009 0.72 -0.2 328,260 + GFF1009 0.72 -1.1 328,260 + GFF1009 0.72 +1.1 328,260 + GFF1009 0.72 -0.2 328,261 - GFF1009 0.72 +1.0 328,261 - GFF1009 0.72 -0.2 328,261 - GFF1009 0.72 -0.4 328,261 - GFF1009 0.72 -0.8 328,261 - GFF1009 0.72 +0.5 328,261 - GFF1009 0.72 +0.7 328,261 - GFF1009 0.72 +0.3 328,261 - GFF1009 0.72 -0.2 328,261 - GFF1009 0.72 +0.6 328,261 - GFF1009 0.72 -1.1 328,261 - GFF1009 0.72 -0.2 328,261 - GFF1009 0.72 +0.0 328,610 + -0.4 328,610 + -1.0 328,610 + +1.9 328,610 + +0.2 328,610 + -0.1 328,610 + -0.9 328,611 - +0.5 328,611 - +0.0 328,611 - +0.1 328,611 - -0.0 328,611 - +0.3 328,940 + GFF1010 0.15 -0.4 328,940 + GFF1010 0.15 +0.2 328,940 + GFF1010 0.15 -1.7 328,940 + GFF1010 0.15 +0.0 328,940 + GFF1010 0.15 +2.1 328,940 + GFF1010 0.15 -0.1 328,940 + GFF1010 0.15 +0.0 328,940 + GFF1010 0.15 -0.2 328,940 + GFF1010 0.15 +0.5 328,941 - GFF1010 0.15 -2.3 328,941 - GFF1010 0.15 +0.1 328,941 - GFF1010 0.15 -1.6 328,941 - GFF1010 0.15 +0.8 328,941 - GFF1010 0.15 +0.2 328,941 - GFF1010 0.15 +0.0 328,941 - GFF1010 0.15 -0.4 328,941 - GFF1010 0.15 -0.7 328,941 - GFF1010 0.15 +0.4 328,941 - GFF1010 0.15 -1.2 329,054 + GFF1010 0.20 -0.5 329,054 + GFF1010 0.20 -0.1 329,054 + GFF1010 0.20 -0.5 329,054 + GFF1010 0.20 -0.2 329,054 + GFF1010 0.20 +1.9 329,054 + GFF1010 0.20 +0.0 329,054 + GFF1010 0.20 -0.4 329,055 - GFF1010 0.20 -0.1 329,055 - GFF1010 0.20 -0.6 329,258 + GFF1010 0.29 -0.2 329,258 + GFF1010 0.29 +1.0 329,258 + GFF1010 0.29 -1.0 329,258 + GFF1010 0.29 +1.1 329,258 + GFF1010 0.29 +0.4 329,258 + GFF1010 0.29 -0.9 329,258 + GFF1010 0.29 +0.4 329,258 + GFF1010 0.29 -1.0 329,258 + GFF1010 0.29 +0.2 329,258 + GFF1010 0.29 +0.9 329,258 + GFF1010 0.29 +0.7 329,258 + GFF1010 0.29 -0.2 329,258 + GFF1010 0.29 -1.4 329,258 + GFF1010 0.29 +0.5 329,259 - GFF1010 0.29 -0.1 329,259 - GFF1010 0.29 +1.8 329,259 - GFF1010 0.29 +0.8 329,259 - GFF1010 0.29 -0.3 329,259 - GFF1010 0.29 +0.7 329,259 - GFF1010 0.29 -0.2 329,259 - GFF1010 0.29 -1.4 329,259 - GFF1010 0.29 -0.8 329,259 - GFF1010 0.29 -1.6 329,259 - GFF1010 0.29 -0.1 329,259 - GFF1010 0.29 -0.1 329,259 - GFF1010 0.29 -0.2 329,259 - GFF1010 0.29 -0.9 329,259 - GFF1010 0.29 +0.0 329,259 - GFF1010 0.29 +0.1 329,259 - GFF1010 0.29 -0.6 329,259 - GFF1010 0.29 +0.7 329,259 - GFF1010 0.29 +2.9 329,285 + GFF1010 0.30 -0.1 329,286 - GFF1010 0.30 -1.8 329,286 - GFF1010 0.30 -1.2
Or see this region's nucleotide sequence