Strain Fitness in Variovorax sp. SCN45 around GFF85

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF83 and GFF84 are separated by 112 nucleotidesGFF84 and GFF85 are separated by 131 nucleotidesGFF85 and GFF86 are separated by 41 nucleotidesGFF86 and GFF87 overlap by 4 nucleotides GFF83 - Alkanal monooxygenase alpha chain (EC 1.14.14.3), at 99,554 to 100,615 GFF83 GFF84 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 100,728 to 101,444 GFF84 GFF85 - Transcriptional regulator, LysR family, at 101,576 to 102,475 GFF85 GFF86 - Cell morphology protein, at 102,517 to 103,191 GFF86 GFF87 - no description, at 103,188 to 103,868 GFF87 Position (kb) 101 102 103Strain fitness (log2 ratio) -2 -1 0 1 2at 100.695 kb on + strandat 100.695 kb on + strandat 100.695 kb on + strandat 100.695 kb on + strandat 100.696 kb on - strandat 100.757 kb on - strandat 101.110 kb on + strand, within GFF84at 101.110 kb on + strand, within GFF84at 101.111 kb on - strand, within GFF84at 101.420 kb on - strandat 101.470 kb on + strandat 101.470 kb on + strandat 101.471 kb on - strandat 101.486 kb on + strandat 101.486 kb on + strandat 101.487 kb on - strandat 101.487 kb on - strandat 101.777 kb on + strand, within GFF85at 101.777 kb on + strand, within GFF85at 101.777 kb on + strand, within GFF85at 101.777 kb on + strand, within GFF85at 101.778 kb on - strand, within GFF85at 101.778 kb on - strand, within GFF85at 102.098 kb on + strand, within GFF85at 102.099 kb on - strand, within GFF85at 102.444 kb on + strandat 102.444 kb on + strandat 102.482 kb on + strandat 102.483 kb on - strandat 102.483 kb on - strandat 102.589 kb on + strand, within GFF86at 102.590 kb on - strand, within GFF86at 102.590 kb on - strand, within GFF86at 102.590 kb on - strand, within GFF86at 102.590 kb on - strand, within GFF86at 102.590 kb on - strand, within GFF86at 102.670 kb on + strand, within GFF86at 102.968 kb on - strand, within GFF86at 102.980 kb on - strand, within GFF86at 102.980 kb on - strand, within GFF86at 103.273 kb on - strand, within GFF87at 103.273 kb on - strand, within GFF87at 103.405 kb on - strand, within GFF87

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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100,695 + +0.3
100,695 + -0.2
100,695 + +0.8
100,695 + -0.5
100,696 - +0.4
100,757 - -0.6
101,110 + GFF84 0.53 -0.6
101,110 + GFF84 0.53 -0.6
101,111 - GFF84 0.53 -1.6
101,420 - -0.8
101,470 + -0.5
101,470 + -0.4
101,471 - -0.4
101,486 + -0.0
101,486 + -0.2
101,487 - +0.4
101,487 - -1.2
101,777 + GFF85 0.22 +0.0
101,777 + GFF85 0.22 +0.9
101,777 + GFF85 0.22 +0.1
101,777 + GFF85 0.22 +2.4
101,778 - GFF85 0.22 -0.8
101,778 - GFF85 0.22 +0.5
102,098 + GFF85 0.58 -0.6
102,099 - GFF85 0.58 -0.0
102,444 + +1.4
102,444 + -0.7
102,482 + +0.1
102,483 - +0.0
102,483 - -1.0
102,589 + GFF86 0.11 -1.0
102,590 - GFF86 0.11 -0.0
102,590 - GFF86 0.11 -0.6
102,590 - GFF86 0.11 -2.1
102,590 - GFF86 0.11 +0.1
102,590 - GFF86 0.11 -0.9
102,670 + GFF86 0.23 -0.2
102,968 - GFF86 0.67 -0.4
102,980 - GFF86 0.69 -0.5
102,980 - GFF86 0.69 -0.0
103,273 - GFF87 0.12 -2.1
103,273 - GFF87 0.12 +1.2
103,405 - GFF87 0.32 -0.9

Or see this region's nucleotide sequence