Strain Fitness in Variovorax sp. SCN45 around GFF7339

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7337 and GFF7338 overlap by 8 nucleotidesGFF7338 and GFF7339 overlap by 4 nucleotidesGFF7339 and GFF7340 are separated by 4 nucleotides GFF7337 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 6,175 to 7,137 GFF7337 GFF7338 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,130 to 7,957 GFF7338 GFF7339 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,954 to 8,781 GFF7339 GFF7340 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 8,786 to 9,877 GFF7340 Position (kb) 7 8 9Strain fitness (log2 ratio) -2 -1 0 1at 7.239 kb on + strand, within GFF7338at 7.342 kb on - strand, within GFF7338at 7.947 kb on - strandat 8.174 kb on + strand, within GFF7339at 8.175 kb on - strand, within GFF7339at 8.742 kb on - strandat 8.847 kb on + strandat 8.864 kb on + strandat 8.864 kb on + strandat 8.910 kb on + strand, within GFF7340at 8.911 kb on - strand, within GFF7340at 9.322 kb on - strand, within GFF7340at 9.322 kb on - strand, within GFF7340at 9.427 kb on - strand, within GFF7340at 9.427 kb on - strand, within GFF7340at 9.594 kb on + strand, within GFF7340at 9.625 kb on - strand, within GFF7340at 9.625 kb on - strand, within GFF7340

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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7,239 + GFF7338 0.13 +1.7
7,342 - GFF7338 0.26 -0.8
7,947 - -0.1
8,174 + GFF7339 0.27 +1.0
8,175 - GFF7339 0.27 +0.6
8,742 - -0.8
8,847 + -0.6
8,864 + +0.1
8,864 + +1.1
8,910 + GFF7340 0.11 -0.6
8,911 - GFF7340 0.11 -0.8
9,322 - GFF7340 0.49 -0.8
9,322 - GFF7340 0.49 -0.3
9,427 - GFF7340 0.59 -1.5
9,427 - GFF7340 0.59 -2.1
9,594 + GFF7340 0.74 -0.1
9,625 - GFF7340 0.77 +0.1
9,625 - GFF7340 0.77 -0.1

Or see this region's nucleotide sequence