Strain Fitness in Variovorax sp. SCN45 around GFF6925

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6923 and GFF6924 overlap by 4 nucleotidesGFF6924 and GFF6925 are separated by 121 nucleotidesGFF6925 and GFF6926 are separated by 9 nucleotidesGFF6926 and GFF6927 are separated by 87 nucleotides GFF6923 - Acetate permease ActP (cation/acetate symporter), at 1,595 to 3,331 GFF6923 GFF6924 - protein of unknown function DUF485, at 3,328 to 3,645 GFF6924 GFF6925 - Acetyl-CoA synthetase (EC 6.2.1.1), at 3,767 to 5,743 GFF6925 GFF6926 - MBL-fold metallo-hydrolase superfamily, at 5,753 to 6,646 GFF6926 GFF6927 - Transcriptional regulator, ArsR family, at 6,734 to 7,057 GFF6927 Position (kb) 3 4 5 6Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2.817 kb on + strand, within GFF6923at 2.818 kb on - strand, within GFF6923at 2.982 kb on + strand, within GFF6923at 2.982 kb on + strand, within GFF6923at 2.983 kb on - strand, within GFF6923at 2.997 kb on + strand, within GFF6923at 3.051 kb on + strand, within GFF6923at 3.052 kb on - strand, within GFF6923at 3.052 kb on - strand, within GFF6923at 3.052 kb on - strand, within GFF6923at 3.109 kb on - strand, within GFF6923at 3.109 kb on - strand, within GFF6923at 3.109 kb on - strand, within GFF6923at 3.109 kb on - strand, within GFF6923at 3.404 kb on + strand, within GFF6924at 3.404 kb on + strand, within GFF6924at 3.405 kb on - strand, within GFF6924at 3.405 kb on - strand, within GFF6924at 3.407 kb on + strand, within GFF6924at 3.407 kb on + strand, within GFF6924at 3.408 kb on - strand, within GFF6924at 3.408 kb on - strand, within GFF6924at 3.408 kb on - strand, within GFF6924at 3.536 kb on + strand, within GFF6924at 3.803 kb on + strandat 3.804 kb on - strandat 3.980 kb on + strand, within GFF6925at 4.109 kb on + strand, within GFF6925at 4.109 kb on + strand, within GFF6925at 4.193 kb on + strand, within GFF6925at 4.193 kb on + strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.194 kb on - strand, within GFF6925at 4.566 kb on - strand, within GFF6925at 4.721 kb on + strand, within GFF6925at 4.721 kb on + strand, within GFF6925at 4.721 kb on + strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.722 kb on - strand, within GFF6925at 4.784 kb on + strand, within GFF6925at 4.841 kb on + strand, within GFF6925at 4.841 kb on + strand, within GFF6925at 5.252 kb on + strand, within GFF6925at 5.809 kb on - strandat 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.039 kb on + strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.040 kb on - strand, within GFF6926at 6.087 kb on + strand, within GFF6926at 6.087 kb on + strand, within GFF6926at 6.186 kb on + strand, within GFF6926at 6.187 kb on - strand, within GFF6926at 6.478 kb on - strand, within GFF6926at 6.478 kb on - strand, within GFF6926at 6.478 kb on - strand, within GFF6926at 6.531 kb on + strand, within GFF6926at 6.532 kb on - strand, within GFF6926at 6.588 kb on + strandat 6.588 kb on + strandat 6.588 kb on + strandat 6.588 kb on + strandat 6.588 kb on + strandat 6.588 kb on + strandat 6.589 kb on - strandat 6.589 kb on - strandat 6.589 kb on - strandat 6.589 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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2,817 + GFF6923 0.70 -0.1
2,818 - GFF6923 0.70 +1.2
2,982 + GFF6923 0.80 -0.1
2,982 + GFF6923 0.80 -0.3
2,983 - GFF6923 0.80 -0.1
2,997 + GFF6923 0.81 +1.0
3,051 + GFF6923 0.84 -1.5
3,052 - GFF6923 0.84 -0.4
3,052 - GFF6923 0.84 -0.2
3,052 - GFF6923 0.84 -0.3
3,109 - GFF6923 0.87 -1.3
3,109 - GFF6923 0.87 +0.7
3,109 - GFF6923 0.87 +0.3
3,109 - GFF6923 0.87 +0.9
3,404 + GFF6924 0.24 -1.8
3,404 + GFF6924 0.24 +0.7
3,405 - GFF6924 0.24 -0.0
3,405 - GFF6924 0.24 -0.7
3,407 + GFF6924 0.25 +0.6
3,407 + GFF6924 0.25 +0.0
3,408 - GFF6924 0.25 -1.1
3,408 - GFF6924 0.25 +0.5
3,408 - GFF6924 0.25 -0.6
3,536 + GFF6924 0.65 +0.0
3,803 + -0.9
3,804 - -1.0
3,980 + GFF6925 0.11 -0.4
4,109 + GFF6925 0.17 +0.5
4,109 + GFF6925 0.17 +0.3
4,193 + GFF6925 0.22 +0.3
4,193 + GFF6925 0.22 -0.3
4,194 - GFF6925 0.22 -0.1
4,194 - GFF6925 0.22 +1.0
4,194 - GFF6925 0.22 -0.7
4,194 - GFF6925 0.22 -0.3
4,194 - GFF6925 0.22 +0.6
4,194 - GFF6925 0.22 +0.3
4,194 - GFF6925 0.22 +0.6
4,566 - GFF6925 0.40 +0.6
4,721 + GFF6925 0.48 -0.6
4,721 + GFF6925 0.48 -0.3
4,721 + GFF6925 0.48 +0.3
4,722 - GFF6925 0.48 +0.4
4,722 - GFF6925 0.48 +0.3
4,722 - GFF6925 0.48 -1.0
4,722 - GFF6925 0.48 -0.1
4,722 - GFF6925 0.48 +0.6
4,722 - GFF6925 0.48 -0.3
4,784 + GFF6925 0.51 +0.3
4,841 + GFF6925 0.54 -0.4
4,841 + GFF6925 0.54 +0.9
5,252 + GFF6925 0.75 -0.7
5,809 - +1.1
6,039 + GFF6926 0.32 +0.8
6,039 + GFF6926 0.32 -0.3
6,039 + GFF6926 0.32 -0.1
6,039 + GFF6926 0.32 -0.6
6,039 + GFF6926 0.32 +0.2
6,039 + GFF6926 0.32 +0.1
6,039 + GFF6926 0.32 -0.1
6,039 + GFF6926 0.32 +0.3
6,040 - GFF6926 0.32 +0.0
6,040 - GFF6926 0.32 +0.4
6,040 - GFF6926 0.32 +0.6
6,040 - GFF6926 0.32 -0.9
6,040 - GFF6926 0.32 +3.1
6,040 - GFF6926 0.32 -0.5
6,040 - GFF6926 0.32 +1.9
6,040 - GFF6926 0.32 -1.0
6,087 + GFF6926 0.37 -0.3
6,087 + GFF6926 0.37 -0.1
6,186 + GFF6926 0.48 -0.4
6,187 - GFF6926 0.49 -0.4
6,478 - GFF6926 0.81 +0.0
6,478 - GFF6926 0.81 -1.9
6,478 - GFF6926 0.81 +0.2
6,531 + GFF6926 0.87 +0.1
6,532 - GFF6926 0.87 +0.2
6,588 + -0.6
6,588 + -0.8
6,588 + +0.3
6,588 + -0.5
6,588 + +0.7
6,588 + -0.9
6,589 - -0.1
6,589 - +0.2
6,589 - +0.6
6,589 - -0.2

Or see this region's nucleotide sequence