Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6654 and GFF6655 are separated by 18 nucleotides GFF6655 and GFF6656 are separated by 341 nucleotides GFF6656 and GFF6657 are separated by 60 nucleotides
GFF6654 - Transcriptional regulator, RpiR family, at 28,085 to 28,951
GFF6654
GFF6655 - Transcriptional regulator, GntR family, at 28,970 to 29,665
GFF6655
GFF6656 - hypothetical protein, at 30,007 to 30,372
GFF6656
GFF6657 - Outer membrane factor (OMF) lipoprotein associated wth MdtABC efflux system, at 30,433 to 31,926
GFF6657
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 28.154 kb on + strand at 28.154 kb on + strand at 28.154 kb on + strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.464 kb on + strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.539 kb on - strand, within GFF6654 at 28.539 kb on - strand, within GFF6654 at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.145 kb on + strand, within GFF6655 at 29.146 kb on - strand, within GFF6655 at 29.146 kb on - strand, within GFF6655 at 29.146 kb on - strand, within GFF6655 at 29.146 kb on - strand, within GFF6655 at 29.300 kb on + strand, within GFF6655 at 29.300 kb on + strand, within GFF6655 at 29.301 kb on - strand, within GFF6655 at 29.301 kb on - strand, within GFF6655 at 29.301 kb on - strand, within GFF6655 at 29.508 kb on + strand, within GFF6655 at 29.508 kb on + strand, within GFF6655 at 29.508 kb on + strand, within GFF6655 at 29.508 kb on + strand, within GFF6655 at 29.508 kb on + strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.509 kb on - strand, within GFF6655 at 29.650 kb on + strand at 29.650 kb on + strand at 29.651 kb on - strand at 29.651 kb on - strand at 29.651 kb on - strand at 29.744 kb on + strand at 29.744 kb on + strand at 29.745 kb on - strand at 29.745 kb on - strand at 29.768 kb on - strand at 29.813 kb on + strand at 29.813 kb on + strand at 29.813 kb on + strand at 29.814 kb on - strand at 29.814 kb on - strand at 29.814 kb on - strand at 29.814 kb on - strand at 29.814 kb on - strand at 29.814 kb on - strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.861 kb on + strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.862 kb on - strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.878 kb on + strand at 29.879 kb on - strand at 29.879 kb on - strand at 29.879 kb on - strand at 29.879 kb on - strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.881 kb on + strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.882 kb on - strand at 29.907 kb on + strand at 29.908 kb on - strand at 29.933 kb on + strand at 29.933 kb on + strand at 29.933 kb on + strand at 29.934 kb on - strand at 29.956 kb on + strand at 29.999 kb on + strand at 30.000 kb on - strand at 30.000 kb on - strand at 30.337 kb on + strand at 30.337 kb on + strand at 30.337 kb on + strand at 30.337 kb on + strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.338 kb on - strand at 30.633 kb on - strand, within GFF6657 at 30.633 kb on - strand, within GFF6657 at 30.633 kb on - strand, within GFF6657 at 30.633 kb on - strand, within GFF6657 at 30.633 kb on - strand, within GFF6657 at 30.654 kb on - strand, within GFF6657 at 30.654 kb on - strand, within GFF6657
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 28,154 + +0.1 28,154 + +0.2 28,154 + +0.9 28,155 - -0.4 28,155 - +0.4 28,155 - +2.0 28,155 - -1.1 28,155 - -0.8 28,155 - +0.0 28,325 + GFF6654 0.28 -1.1 28,325 + GFF6654 0.28 -0.3 28,325 + GFF6654 0.28 -0.7 28,325 + GFF6654 0.28 +0.3 28,326 - GFF6654 0.28 +0.5 28,326 - GFF6654 0.28 -0.2 28,326 - GFF6654 0.28 +0.7 28,326 - GFF6654 0.28 -1.5 28,326 - GFF6654 0.28 -0.7 28,326 - GFF6654 0.28 -1.2 28,326 - GFF6654 0.28 -0.0 28,326 - GFF6654 0.28 -0.2 28,326 - GFF6654 0.28 +0.2 28,326 - GFF6654 0.28 -0.5 28,326 - GFF6654 0.28 +0.2 28,326 - GFF6654 0.28 +0.1 28,326 - GFF6654 0.28 +0.5 28,464 + GFF6654 0.44 -1.6 28,465 - GFF6654 0.44 +0.4 28,465 - GFF6654 0.44 -0.1 28,465 - GFF6654 0.44 +0.5 28,532 + GFF6654 0.52 -0.3 28,532 + GFF6654 0.52 -0.5 28,532 + GFF6654 0.52 +0.5 28,532 + GFF6654 0.52 -0.3 28,532 + GFF6654 0.52 -0.6 28,532 + GFF6654 0.52 +0.5 28,532 + GFF6654 0.52 -1.3 28,532 + GFF6654 0.52 -0.9 28,532 + GFF6654 0.52 -0.4 28,532 + GFF6654 0.52 -0.9 28,532 + GFF6654 0.52 -0.2 28,532 + GFF6654 0.52 -0.2 28,532 + GFF6654 0.52 -0.3 28,533 - GFF6654 0.52 -0.7 28,533 - GFF6654 0.52 -0.9 28,533 - GFF6654 0.52 -0.5 28,533 - GFF6654 0.52 +0.2 28,533 - GFF6654 0.52 -0.1 28,533 - GFF6654 0.52 +0.1 28,533 - GFF6654 0.52 -0.5 28,533 - GFF6654 0.52 +0.2 28,533 - GFF6654 0.52 -0.4 28,533 - GFF6654 0.52 +0.2 28,538 + GFF6654 0.52 -0.9 28,538 + GFF6654 0.52 +0.9 28,538 + GFF6654 0.52 -1.5 28,539 - GFF6654 0.52 -0.9 28,539 - GFF6654 0.52 -2.3 28,919 + -0.4 28,919 + -0.9 28,919 + -0.6 28,919 + -0.1 28,919 + -1.0 28,919 + -0.3 28,919 + +1.5 28,919 + -0.1 28,920 - -0.5 28,920 - -0.9 28,920 - +0.2 28,920 - +0.3 28,920 - -0.2 28,920 - -0.4 28,920 - -0.3 28,920 - -0.5 28,920 - +0.8 28,920 - -1.1 28,920 - +0.3 29,145 + GFF6655 0.25 -0.0 29,145 + GFF6655 0.25 -0.6 29,145 + GFF6655 0.25 +1.5 29,145 + GFF6655 0.25 +0.5 29,145 + GFF6655 0.25 +0.4 29,145 + GFF6655 0.25 -0.1 29,145 + GFF6655 0.25 +0.9 29,146 - GFF6655 0.25 -0.9 29,146 - GFF6655 0.25 +0.0 29,146 - GFF6655 0.25 -2.9 29,146 - GFF6655 0.25 -0.8 29,300 + GFF6655 0.47 -0.0 29,300 + GFF6655 0.47 -1.3 29,301 - GFF6655 0.48 +0.4 29,301 - GFF6655 0.48 +0.5 29,301 - GFF6655 0.48 +0.4 29,508 + GFF6655 0.77 -0.2 29,508 + GFF6655 0.77 -0.4 29,508 + GFF6655 0.77 +0.1 29,508 + GFF6655 0.77 -0.8 29,508 + GFF6655 0.77 -0.6 29,509 - GFF6655 0.77 -0.8 29,509 - GFF6655 0.77 -1.3 29,509 - GFF6655 0.77 +1.8 29,509 - GFF6655 0.77 -0.2 29,509 - GFF6655 0.77 +0.2 29,509 - GFF6655 0.77 -0.5 29,509 - GFF6655 0.77 +0.3 29,509 - GFF6655 0.77 +1.5 29,509 - GFF6655 0.77 +0.3 29,650 + -0.1 29,650 + +0.2 29,651 - -0.6 29,651 - +0.5 29,651 - +0.1 29,744 + -0.7 29,744 + -1.6 29,745 - -0.4 29,745 - -0.7 29,768 - -1.0 29,813 + +0.1 29,813 + -0.5 29,813 + -0.8 29,814 - -0.2 29,814 - -1.0 29,814 - -0.7 29,814 - +0.7 29,814 - -0.2 29,814 - -0.6 29,861 + -1.5 29,861 + -0.1 29,861 + -0.0 29,861 + -0.5 29,861 + -0.9 29,861 + +0.7 29,861 + +0.9 29,861 + +0.3 29,861 + -0.7 29,861 + -0.9 29,861 + +0.3 29,861 + -0.3 29,861 + -0.2 29,861 + -0.5 29,861 + -0.9 29,861 + +0.1 29,862 - -0.0 29,862 - -0.2 29,862 - -1.1 29,862 - +0.3 29,862 - +0.1 29,862 - -0.0 29,862 - +2.5 29,862 - -0.8 29,862 - -0.3 29,862 - -2.5 29,862 - +0.2 29,862 - +0.4 29,862 - +1.4 29,862 - +0.7 29,862 - +0.8 29,862 - -0.3 29,862 - -0.1 29,862 - -0.2 29,862 - +0.7 29,878 + -0.7 29,878 + -0.3 29,878 + +0.4 29,878 + +0.0 29,878 + -1.2 29,878 + -0.6 29,878 + -1.1 29,879 - -0.7 29,879 - -0.9 29,879 - -0.4 29,879 - -0.8 29,881 + -1.9 29,881 + -0.1 29,881 + -0.1 29,881 + +1.0 29,881 + -0.4 29,881 + -1.5 29,881 + +0.1 29,881 + -1.2 29,881 + -0.6 29,881 + -0.5 29,881 + -1.0 29,881 + +0.4 29,881 + +0.1 29,881 + -0.9 29,881 + -1.1 29,881 + -1.2 29,881 + +1.1 29,881 + -0.1 29,881 + -0.2 29,881 + +0.0 29,882 - -0.0 29,882 - -0.1 29,882 - +0.6 29,882 - -1.6 29,882 - -1.1 29,882 - -0.5 29,882 - -0.7 29,882 - -1.8 29,882 - +0.5 29,882 - -0.2 29,882 - -0.6 29,882 - -0.7 29,882 - +0.3 29,907 + +0.8 29,908 - -0.3 29,933 + -1.3 29,933 + +0.4 29,933 + -0.8 29,934 - -0.6 29,956 + +0.9 29,999 + +0.8 30,000 - +0.4 30,000 - -0.0 30,337 + -0.4 30,337 + -0.3 30,337 + +0.5 30,337 + -1.6 30,338 - -1.3 30,338 - +0.0 30,338 - +0.8 30,338 - +0.8 30,338 - -0.3 30,338 - +0.7 30,338 - -0.1 30,633 - GFF6657 0.13 -0.6 30,633 - GFF6657 0.13 -0.1 30,633 - GFF6657 0.13 +1.5 30,633 - GFF6657 0.13 -0.1 30,633 - GFF6657 0.13 -0.2 30,654 - GFF6657 0.15 -1.2 30,654 - GFF6657 0.15 -1.5
Or see this region's nucleotide sequence