Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6453 and GFF6454 are separated by 17 nucleotides GFF6454 and GFF6455 are separated by 176 nucleotides GFF6455 and GFF6456 are separated by 7 nucleotides
GFF6453 - Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein, at 30,177 to 31,949
GFF6453
GFF6454 - Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, gamma subunit, at 31,967 to 32,713
GFF6454
GFF6455 - Bll3817 protein, at 32,890 to 33,474
GFF6455
GFF6456 - Lipid A phosphoethanolamine transferase, putative, at 33,482 to 35,233
GFF6456
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 32.007 kb on + strand at 32.007 kb on + strand at 32.007 kb on + strand at 32.008 kb on - strand at 32.008 kb on - strand at 32.008 kb on - strand at 32.008 kb on - strand at 32.121 kb on + strand, within GFF6454 at 32.122 kb on - strand, within GFF6454 at 32.277 kb on + strand, within GFF6454 at 32.277 kb on + strand, within GFF6454 at 32.277 kb on + strand, within GFF6454 at 32.278 kb on - strand, within GFF6454 at 32.278 kb on - strand, within GFF6454 at 32.338 kb on - strand, within GFF6454 at 32.379 kb on + strand, within GFF6454 at 32.379 kb on + strand, within GFF6454 at 32.379 kb on + strand, within GFF6454 at 32.379 kb on + strand, within GFF6454 at 32.380 kb on - strand, within GFF6454 at 32.380 kb on - strand, within GFF6454 at 32.380 kb on - strand, within GFF6454 at 32.380 kb on - strand, within GFF6454 at 32.380 kb on - strand, within GFF6454 at 32.529 kb on + strand, within GFF6454 at 32.529 kb on + strand, within GFF6454 at 32.529 kb on + strand, within GFF6454 at 32.541 kb on + strand, within GFF6454 at 32.541 kb on + strand, within GFF6454 at 32.542 kb on - strand, within GFF6454 at 32.542 kb on - strand, within GFF6454 at 32.542 kb on - strand, within GFF6454 at 32.542 kb on - strand, within GFF6454 at 32.697 kb on + strand at 32.698 kb on - strand at 32.719 kb on + strand at 32.720 kb on - strand at 32.720 kb on - strand at 32.720 kb on - strand at 32.720 kb on - strand at 32.787 kb on + strand at 32.787 kb on + strand at 32.787 kb on + strand at 32.787 kb on + strand at 32.787 kb on + strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.788 kb on - strand at 32.804 kb on + strand at 32.804 kb on + strand at 32.805 kb on - strand at 32.805 kb on - strand at 32.805 kb on - strand at 33.046 kb on + strand, within GFF6455 at 33.143 kb on - strand, within GFF6455 at 33.143 kb on - strand, within GFF6455 at 33.178 kb on + strand, within GFF6455 at 33.179 kb on - strand, within GFF6455 at 33.220 kb on + strand, within GFF6455 at 33.274 kb on + strand, within GFF6455 at 33.275 kb on - strand, within GFF6455 at 33.473 kb on - strand at 33.473 kb on - strand at 33.473 kb on - strand at 33.473 kb on - strand at 33.473 kb on - strand at 33.528 kb on + strand at 33.528 kb on + strand at 33.528 kb on + strand at 33.529 kb on - strand at 33.571 kb on - strand at 33.571 kb on - strand at 33.571 kb on - strand at 33.664 kb on + strand, within GFF6456 at 33.665 kb on - strand, within GFF6456 at 33.706 kb on - strand, within GFF6456 at 33.732 kb on + strand, within GFF6456 at 33.765 kb on + strand, within GFF6456 at 33.765 kb on + strand, within GFF6456 at 33.766 kb on - strand, within GFF6456 at 33.783 kb on + strand, within GFF6456 at 33.783 kb on + strand, within GFF6456 at 33.783 kb on + strand, within GFF6456 at 33.783 kb on + strand, within GFF6456 at 33.784 kb on - strand, within GFF6456 at 33.784 kb on - strand, within GFF6456 at 33.784 kb on - strand, within GFF6456 at 33.859 kb on - strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.951 kb on + strand, within GFF6456 at 33.952 kb on - strand, within GFF6456 at 33.952 kb on - strand, within GFF6456 at 33.955 kb on - strand, within GFF6456 at 33.955 kb on - strand, within GFF6456 at 34.230 kb on + strand, within GFF6456 at 34.231 kb on - strand, within GFF6456 at 34.231 kb on - strand, within GFF6456 at 34.251 kb on + strand, within GFF6456 at 34.458 kb on + strand, within GFF6456 at 34.459 kb on - strand, within GFF6456
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 32,007 + +0.3 32,007 + +0.5 32,007 + -0.1 32,008 - -0.6 32,008 - +0.6 32,008 - -0.7 32,008 - +0.1 32,121 + GFF6454 0.21 -0.1 32,122 - GFF6454 0.21 +0.2 32,277 + GFF6454 0.41 -0.4 32,277 + GFF6454 0.41 -1.1 32,277 + GFF6454 0.41 -0.5 32,278 - GFF6454 0.42 -0.8 32,278 - GFF6454 0.42 -1.8 32,338 - GFF6454 0.50 -0.0 32,379 + GFF6454 0.55 +0.3 32,379 + GFF6454 0.55 -0.2 32,379 + GFF6454 0.55 -0.1 32,379 + GFF6454 0.55 -1.2 32,380 - GFF6454 0.55 +0.1 32,380 - GFF6454 0.55 +1.2 32,380 - GFF6454 0.55 -1.9 32,380 - GFF6454 0.55 -0.0 32,380 - GFF6454 0.55 +0.6 32,529 + GFF6454 0.75 -0.9 32,529 + GFF6454 0.75 +1.3 32,529 + GFF6454 0.75 -1.5 32,541 + GFF6454 0.77 -0.7 32,541 + GFF6454 0.77 -0.0 32,542 - GFF6454 0.77 +0.1 32,542 - GFF6454 0.77 -0.6 32,542 - GFF6454 0.77 -0.5 32,542 - GFF6454 0.77 -1.2 32,697 + +0.6 32,698 - -0.6 32,719 + -0.1 32,720 - +0.0 32,720 - +0.1 32,720 - -0.3 32,720 - -0.7 32,787 + -0.8 32,787 + +0.0 32,787 + -0.4 32,787 + -1.9 32,787 + -0.0 32,788 - -0.9 32,788 - +0.2 32,788 - -1.7 32,788 - +0.9 32,788 - +0.1 32,788 - +1.3 32,788 - -0.7 32,804 + +0.3 32,804 + +0.2 32,805 - -0.9 32,805 - -0.9 32,805 - -0.7 33,046 + GFF6455 0.27 -1.9 33,143 - GFF6455 0.43 -0.8 33,143 - GFF6455 0.43 +1.4 33,178 + GFF6455 0.49 -0.5 33,179 - GFF6455 0.49 -0.3 33,220 + GFF6455 0.56 +1.1 33,274 + GFF6455 0.66 -0.9 33,275 - GFF6455 0.66 -1.6 33,473 - -0.6 33,473 - -0.1 33,473 - +0.1 33,473 - -0.7 33,473 - -0.8 33,528 + -1.1 33,528 + +0.1 33,528 + -0.5 33,529 - -0.4 33,571 - +1.2 33,571 - -0.4 33,571 - -0.5 33,664 + GFF6456 0.10 -0.9 33,665 - GFF6456 0.10 -0.7 33,706 - GFF6456 0.13 -1.4 33,732 + GFF6456 0.14 -0.8 33,765 + GFF6456 0.16 +0.6 33,765 + GFF6456 0.16 +0.3 33,766 - GFF6456 0.16 -0.1 33,783 + GFF6456 0.17 +0.6 33,783 + GFF6456 0.17 -0.7 33,783 + GFF6456 0.17 +0.6 33,783 + GFF6456 0.17 +0.1 33,784 - GFF6456 0.17 +0.1 33,784 - GFF6456 0.17 -0.1 33,784 - GFF6456 0.17 -0.2 33,859 - GFF6456 0.22 +1.1 33,951 + GFF6456 0.27 -0.8 33,951 + GFF6456 0.27 -0.2 33,951 + GFF6456 0.27 -0.4 33,951 + GFF6456 0.27 -0.5 33,951 + GFF6456 0.27 +0.1 33,951 + GFF6456 0.27 +2.1 33,951 + GFF6456 0.27 -0.5 33,952 - GFF6456 0.27 -1.1 33,952 - GFF6456 0.27 -0.7 33,955 - GFF6456 0.27 +0.9 33,955 - GFF6456 0.27 +1.6 34,230 + GFF6456 0.43 -0.5 34,231 - GFF6456 0.43 -0.7 34,231 - GFF6456 0.43 -0.4 34,251 + GFF6456 0.44 -0.4 34,458 + GFF6456 0.56 -0.1 34,459 - GFF6456 0.56 -0.5
Or see this region's nucleotide sequence