Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5468 and GFF5469 are separated by 129 nucleotides GFF5469 and GFF5470 are separated by 7 nucleotides GFF5470 and GFF5471 are separated by 11 nucleotides GFF5471 and GFF5472 are separated by 11 nucleotides GFF5472 and GFF5473 are separated by 29 nucleotides
GFF5468 - FAD/FMN-containing dehydrogenase, at 97,775 to 99,157
GFF5468
GFF5469 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 99,287 to 100,141
GFF5469
GFF5470 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 100,149 to 100,952
GFF5470
GFF5471 - Creatinine amidohydrolase (EC 3.5.2.10), at 100,964 to 101,785
GFF5471
GFF5472 - SEED: DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 101,797 to 101,910
GFF5472
GFF5473 - hypothetical protein, at 101,940 to 104,396
GFF5473
Position (kb)
100
101 Strain fitness (log2 ratio)
-2
-1
0
1 at 99.237 kb on - strand at 99.288 kb on + strand at 99.288 kb on + strand at 99.289 kb on - strand at 99.289 kb on - strand at 99.549 kb on + strand, within GFF5469 at 99.549 kb on + strand, within GFF5469 at 99.550 kb on - strand, within GFF5469 at 99.550 kb on - strand, within GFF5469 at 99.550 kb on - strand, within GFF5469 at 99.550 kb on - strand, within GFF5469 at 99.576 kb on + strand, within GFF5469 at 99.576 kb on + strand, within GFF5469 at 99.577 kb on - strand, within GFF5469 at 99.577 kb on - strand, within GFF5469 at 99.606 kb on + strand, within GFF5469 at 99.606 kb on + strand, within GFF5469 at 99.606 kb on + strand, within GFF5469 at 99.606 kb on + strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.607 kb on - strand, within GFF5469 at 99.732 kb on + strand, within GFF5469 at 99.732 kb on + strand, within GFF5469 at 99.732 kb on + strand, within GFF5469 at 99.732 kb on + strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.733 kb on - strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.972 kb on + strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 99.973 kb on - strand, within GFF5469 at 100.219 kb on + strand at 100.219 kb on + strand at 100.220 kb on - strand at 100.256 kb on - strand, within GFF5470 at 100.330 kb on + strand, within GFF5470 at 100.330 kb on + strand, within GFF5470 at 100.330 kb on + strand, within GFF5470 at 100.331 kb on - strand, within GFF5470 at 100.331 kb on - strand, within GFF5470 at 100.331 kb on - strand, within GFF5470 at 100.331 kb on - strand, within GFF5470 at 100.342 kb on + strand, within GFF5470 at 100.342 kb on + strand, within GFF5470 at 100.343 kb on - strand, within GFF5470 at 100.343 kb on - strand, within GFF5470 at 100.537 kb on + strand, within GFF5470 at 100.538 kb on - strand, within GFF5470 at 100.538 kb on - strand, within GFF5470 at 100.965 kb on + strand at 100.966 kb on - strand at 101.232 kb on + strand, within GFF5471 at 101.232 kb on + strand, within GFF5471 at 101.233 kb on - strand, within GFF5471 at 101.233 kb on - strand, within GFF5471 at 101.337 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.430 kb on + strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.431 kb on - strand, within GFF5471 at 101.821 kb on + strand, within GFF5472 at 101.822 kb on - strand, within GFF5472 at 101.827 kb on - strand, within GFF5472 at 101.855 kb on + strand, within GFF5472 at 101.855 kb on + strand, within GFF5472 at 101.855 kb on + strand, within GFF5472 at 101.856 kb on - strand, within GFF5472 at 101.860 kb on - strand, within GFF5472 at 101.885 kb on + strand, within GFF5472 at 101.885 kb on + strand, within GFF5472 at 101.885 kb on + strand, within GFF5472 at 101.885 kb on + strand, within GFF5472 at 101.885 kb on + strand, within GFF5472 at 101.886 kb on - strand, within GFF5472 at 101.886 kb on - strand, within GFF5472 at 101.886 kb on - strand, within GFF5472 at 101.886 kb on - strand, within GFF5472 at 101.941 kb on + strand at 101.942 kb on - strand at 101.942 kb on - strand at 101.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 99,237 - +0.0 99,288 + +1.0 99,288 + -0.1 99,289 - -0.1 99,289 - -0.9 99,549 + GFF5469 0.31 -0.4 99,549 + GFF5469 0.31 +0.6 99,550 - GFF5469 0.31 +0.1 99,550 - GFF5469 0.31 -0.4 99,550 - GFF5469 0.31 +0.2 99,550 - GFF5469 0.31 +0.5 99,576 + GFF5469 0.34 +0.3 99,576 + GFF5469 0.34 +0.3 99,577 - GFF5469 0.34 +0.6 99,577 - GFF5469 0.34 +0.0 99,606 + GFF5469 0.37 +0.1 99,606 + GFF5469 0.37 +0.2 99,606 + GFF5469 0.37 -0.4 99,606 + GFF5469 0.37 +0.2 99,607 - GFF5469 0.37 -0.6 99,607 - GFF5469 0.37 -1.5 99,607 - GFF5469 0.37 -0.4 99,607 - GFF5469 0.37 -0.2 99,607 - GFF5469 0.37 -0.0 99,607 - GFF5469 0.37 -0.1 99,732 + GFF5469 0.52 +0.2 99,732 + GFF5469 0.52 +0.0 99,732 + GFF5469 0.52 +0.6 99,732 + GFF5469 0.52 -1.7 99,733 - GFF5469 0.52 +1.0 99,733 - GFF5469 0.52 -0.3 99,733 - GFF5469 0.52 +1.2 99,733 - GFF5469 0.52 -0.5 99,733 - GFF5469 0.52 -2.1 99,733 - GFF5469 0.52 -0.8 99,733 - GFF5469 0.52 +0.3 99,733 - GFF5469 0.52 -0.4 99,733 - GFF5469 0.52 -1.5 99,972 + GFF5469 0.80 +0.5 99,972 + GFF5469 0.80 +0.1 99,972 + GFF5469 0.80 +0.7 99,972 + GFF5469 0.80 +0.8 99,972 + GFF5469 0.80 +0.3 99,972 + GFF5469 0.80 -0.5 99,973 - GFF5469 0.80 +0.5 99,973 - GFF5469 0.80 +0.8 99,973 - GFF5469 0.80 -0.1 99,973 - GFF5469 0.80 -0.2 99,973 - GFF5469 0.80 -1.1 99,973 - GFF5469 0.80 -0.1 99,973 - GFF5469 0.80 -0.3 99,973 - GFF5469 0.80 +0.2 99,973 - GFF5469 0.80 -0.3 99,973 - GFF5469 0.80 +0.7 99,973 - GFF5469 0.80 -0.2 99,973 - GFF5469 0.80 +1.0 100,219 + -1.3 100,219 + +1.0 100,220 - -1.4 100,256 - GFF5470 0.13 -1.4 100,330 + GFF5470 0.23 +0.1 100,330 + GFF5470 0.23 +1.0 100,330 + GFF5470 0.23 +0.0 100,331 - GFF5470 0.23 +1.3 100,331 - GFF5470 0.23 +0.2 100,331 - GFF5470 0.23 -1.3 100,331 - GFF5470 0.23 -0.9 100,342 + GFF5470 0.24 -1.2 100,342 + GFF5470 0.24 +0.0 100,343 - GFF5470 0.24 -1.7 100,343 - GFF5470 0.24 +0.5 100,537 + GFF5470 0.48 -1.2 100,538 - GFF5470 0.48 +1.0 100,538 - GFF5470 0.48 +1.3 100,965 + -0.3 100,966 - -1.3 101,232 + GFF5471 0.33 -0.7 101,232 + GFF5471 0.33 +1.2 101,233 - GFF5471 0.33 -0.8 101,233 - GFF5471 0.33 +0.2 101,337 + GFF5471 0.45 -0.3 101,430 + GFF5471 0.57 +0.9 101,430 + GFF5471 0.57 -0.4 101,430 + GFF5471 0.57 +0.6 101,430 + GFF5471 0.57 +0.5 101,430 + GFF5471 0.57 -0.3 101,430 + GFF5471 0.57 +0.3 101,430 + GFF5471 0.57 +1.0 101,431 - GFF5471 0.57 -0.3 101,431 - GFF5471 0.57 +0.5 101,431 - GFF5471 0.57 -1.1 101,431 - GFF5471 0.57 -0.9 101,431 - GFF5471 0.57 -0.1 101,431 - GFF5471 0.57 +0.8 101,431 - GFF5471 0.57 -0.7 101,431 - GFF5471 0.57 -0.1 101,821 + GFF5472 0.21 -0.9 101,822 - GFF5472 0.22 -1.2 101,827 - GFF5472 0.26 -0.1 101,855 + GFF5472 0.51 -0.2 101,855 + GFF5472 0.51 +1.0 101,855 + GFF5472 0.51 -0.3 101,856 - GFF5472 0.52 +0.2 101,860 - GFF5472 0.55 -0.6 101,885 + GFF5472 0.77 +1.4 101,885 + GFF5472 0.77 +0.0 101,885 + GFF5472 0.77 -0.4 101,885 + GFF5472 0.77 +0.0 101,885 + GFF5472 0.77 +0.0 101,886 - GFF5472 0.78 +0.8 101,886 - GFF5472 0.78 -0.8 101,886 - GFF5472 0.78 +0.0 101,886 - GFF5472 0.78 -0.8 101,941 + -0.8 101,942 - +0.5 101,942 - -0.2 101,942 - +0.3
Or see this region's nucleotide sequence