Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5043 and GFF5044 are separated by 4 nucleotides GFF5044 and GFF5045 are separated by 16 nucleotides GFF5045 and GFF5046 are separated by 18 nucleotides GFF5046 and GFF5047 are separated by 28 nucleotides
GFF5043 - ABC transporter, permease protein (cluster 9, phospholipid), at 144,932 to 146,098
GFF5043
GFF5044 - ABC transporter, ATP-binding protein (cluster 9, phospholipid), at 146,103 to 146,948
GFF5044
GFF5045 - ABC transporter, substrate-binding protein (cluster 9, phospholipid), at 146,965 to 147,924
GFF5045
GFF5046 - putative lipoprotein, at 147,943 to 148,656
GFF5046
GFF5047 - hypothetical protein, at 148,685 to 148,906
GFF5047
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1
2 at 146.029 kb on + strand at 146.029 kb on + strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.030 kb on - strand at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.847 kb on + strand, within GFF5044 at 146.848 kb on - strand, within GFF5044 at 146.848 kb on - strand, within GFF5044 at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.953 kb on + strand at 146.954 kb on - strand at 146.954 kb on - strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.957 kb on + strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.958 kb on - strand at 146.969 kb on - strand at 147.076 kb on + strand, within GFF5045 at 147.262 kb on + strand, within GFF5045 at 147.262 kb on + strand, within GFF5045 at 147.262 kb on + strand, within GFF5045 at 147.262 kb on + strand, within GFF5045 at 147.263 kb on - strand, within GFF5045 at 147.263 kb on - strand, within GFF5045 at 147.263 kb on - strand, within GFF5045 at 147.263 kb on - strand, within GFF5045 at 147.737 kb on - strand, within GFF5045 at 148.022 kb on + strand, within GFF5046 at 148.022 kb on + strand, within GFF5046 at 148.022 kb on + strand, within GFF5046 at 148.022 kb on + strand, within GFF5046 at 148.022 kb on + strand, within GFF5046 at 148.022 kb on + strand, within GFF5046 at 148.023 kb on - strand, within GFF5046 at 148.087 kb on + strand, within GFF5046 at 148.088 kb on - strand, within GFF5046 at 148.423 kb on + strand, within GFF5046 at 148.423 kb on + strand, within GFF5046 at 148.424 kb on - strand, within GFF5046 at 148.424 kb on - strand, within GFF5046 at 148.424 kb on - strand, within GFF5046 at 148.654 kb on + strand at 148.654 kb on + strand at 148.655 kb on - strand at 148.655 kb on - strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.679 kb on + strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.680 kb on - strand at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.863 kb on + strand, within GFF5047 at 148.864 kb on - strand, within GFF5047 at 148.864 kb on - strand, within GFF5047 at 148.864 kb on - strand, within GFF5047 at 148.864 kb on - strand, within GFF5047
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 146,029 + -0.1 146,029 + +0.2 146,030 - -0.2 146,030 - +0.0 146,030 - +0.5 146,030 - -0.2 146,847 + GFF5044 0.88 +0.3 146,847 + GFF5044 0.88 +0.3 146,847 + GFF5044 0.88 -0.3 146,847 + GFF5044 0.88 +1.0 146,848 - GFF5044 0.88 +0.2 146,848 - GFF5044 0.88 +0.1 146,953 + -0.2 146,953 + -1.0 146,953 + -1.0 146,953 + +0.1 146,953 + -0.0 146,953 + -0.0 146,954 - +2.6 146,954 - -0.0 146,957 + -0.6 146,957 + -0.8 146,957 + +1.0 146,957 + -0.7 146,957 + -0.1 146,958 - +0.6 146,958 - +0.7 146,958 - +1.6 146,958 - -0.6 146,958 - -0.2 146,958 - +0.7 146,958 - -0.0 146,958 - -0.6 146,958 - +0.2 146,969 - -0.3 147,076 + GFF5045 0.12 +0.2 147,262 + GFF5045 0.31 +0.1 147,262 + GFF5045 0.31 -0.6 147,262 + GFF5045 0.31 -1.5 147,262 + GFF5045 0.31 +1.6 147,263 - GFF5045 0.31 -0.6 147,263 - GFF5045 0.31 +2.0 147,263 - GFF5045 0.31 +0.7 147,263 - GFF5045 0.31 +0.2 147,737 - GFF5045 0.80 -1.5 148,022 + GFF5046 0.11 +0.5 148,022 + GFF5046 0.11 +0.1 148,022 + GFF5046 0.11 -0.6 148,022 + GFF5046 0.11 +1.0 148,022 + GFF5046 0.11 +0.2 148,022 + GFF5046 0.11 +0.1 148,023 - GFF5046 0.11 -1.1 148,087 + GFF5046 0.20 -0.1 148,088 - GFF5046 0.20 -2.3 148,423 + GFF5046 0.67 -0.0 148,423 + GFF5046 0.67 -1.1 148,424 - GFF5046 0.67 +0.2 148,424 - GFF5046 0.67 -0.0 148,424 - GFF5046 0.67 +1.1 148,654 + +0.9 148,654 + -1.0 148,655 - -0.3 148,655 - -1.0 148,679 + -0.7 148,679 + -0.0 148,679 + -0.1 148,679 + -1.2 148,679 + +1.0 148,679 + +0.3 148,679 + +1.1 148,679 + -1.2 148,679 + -1.1 148,679 + +0.3 148,679 + -0.9 148,679 + -0.7 148,679 + +1.0 148,679 + -1.0 148,680 - -0.4 148,680 - +0.4 148,680 - +0.5 148,680 - +0.1 148,680 - -1.5 148,680 - -0.0 148,680 - -0.7 148,680 - -0.5 148,680 - -0.7 148,680 - -0.3 148,680 - -0.2 148,680 - -0.0 148,680 - +0.0 148,680 - +1.0 148,680 - -1.1 148,680 - +0.3 148,863 + GFF5047 0.80 +0.5 148,863 + GFF5047 0.80 -0.1 148,863 + GFF5047 0.80 -0.1 148,863 + GFF5047 0.80 -0.8 148,863 + GFF5047 0.80 -0.5 148,863 + GFF5047 0.80 -0.1 148,863 + GFF5047 0.80 -0.0 148,864 - GFF5047 0.81 +0.4 148,864 - GFF5047 0.81 -0.4 148,864 - GFF5047 0.81 +0.3 148,864 - GFF5047 0.81 +0.7
Or see this region's nucleotide sequence