Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4882 and GFF4884 are separated by 65 nucleotides GFF4884 and GFF4883 overlap by 27 nucleotides GFF4883 and GFF4885 overlap by 1 nucleotides GFF4885 and GFF4886 are separated by 147 nucleotides GFF4886 and GFF4887 are separated by 142 nucleotides
GFF4882 - putative plasmid stabilization protein, at 249,842 to 251,881
GFF4882
GFF4884 - UPF0380 proteins YafZ and homologs, at 251,947 to 252,771
GFF4884
GFF4883 - Translation-disabling ACNase RloC, at 252,745 to 253,371
GFF4883
GFF4885 - FIG00582571: hypothetical protein, at 253,371 to 253,559
GFF4885
GFF4886 - hypothetical protein, at 253,707 to 254,006
GFF4886
GFF4887 - FIG00582580: hypothetical protein, at 254,149 to 254,427
GFF4887
Position (kb) 252
253
254 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.865 kb on + strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.866 kb on - strand at 251.869 kb on + strand at 251.869 kb on + strand at 251.870 kb on - strand at 252.200 kb on + strand, within GFF4884 at 252.200 kb on + strand, within GFF4884 at 252.201 kb on - strand, within GFF4884 at 252.201 kb on - strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.307 kb on + strand, within GFF4884 at 252.308 kb on - strand, within GFF4884 at 252.308 kb on - strand, within GFF4884 at 252.308 kb on - strand, within GFF4884 at 252.308 kb on - strand, within GFF4884 at 252.314 kb on + strand, within GFF4884 at 252.314 kb on + strand, within GFF4884 at 252.314 kb on + strand, within GFF4884 at 252.315 kb on - strand, within GFF4884 at 252.315 kb on - strand, within GFF4884 at 252.438 kb on - strand, within GFF4884 at 252.438 kb on - strand, within GFF4884 at 252.480 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 253.035 kb on + strand, within GFF4883 at 253.035 kb on + strand, within GFF4883 at 253.036 kb on - strand, within GFF4883 at 253.227 kb on + strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.355 kb on - strand at 253.355 kb on - strand at 253.355 kb on - strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.558 kb on - strand at 253.558 kb on - strand at 253.558 kb on - strand at 253.674 kb on + strand at 253.674 kb on + strand at 253.674 kb on + strand at 253.675 kb on - strand at 253.675 kb on - strand at 253.896 kb on + strand, within GFF4886 at 253.896 kb on + strand, within GFF4886 at 253.896 kb on + strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.219 kb on + strand, within GFF4887 at 254.220 kb on - strand, within GFF4887 at 254.220 kb on - strand, within GFF4887 at 254.237 kb on + strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.264 kb on + strand, within GFF4887 at 254.265 kb on - strand, within GFF4887 at 254.265 kb on - strand, within GFF4887 at 254.310 kb on + strand, within GFF4887 at 254.310 kb on + strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 251,865 + -0.3 251,865 + -0.5 251,865 + -0.1 251,865 + -0.5 251,865 + -0.5 251,865 + +0.8 251,865 + +1.0 251,865 + -1.3 251,865 + -0.9 251,865 + -1.1 251,865 + -0.2 251,865 + +0.9 251,865 + -0.6 251,865 + +0.2 251,865 + +0.1 251,866 - +0.3 251,866 - +0.3 251,866 - +0.2 251,866 - +0.5 251,866 - -0.6 251,866 - +1.5 251,866 - -0.7 251,866 - -0.5 251,866 - -0.6 251,866 - -1.4 251,866 - -0.5 251,866 - -0.3 251,866 - -0.8 251,866 - -1.1 251,866 - -0.4 251,869 + -0.8 251,869 + -0.3 251,870 - -0.3 252,200 + GFF4884 0.31 -0.7 252,200 + GFF4884 0.31 +0.7 252,201 - GFF4884 0.31 +0.9 252,201 - GFF4884 0.31 +0.2 252,307 + GFF4884 0.44 +0.3 252,307 + GFF4884 0.44 +1.3 252,307 + GFF4884 0.44 +0.5 252,307 + GFF4884 0.44 -1.8 252,307 + GFF4884 0.44 -0.5 252,307 + GFF4884 0.44 -0.9 252,308 - GFF4884 0.44 -1.3 252,308 - GFF4884 0.44 -0.3 252,308 - GFF4884 0.44 +0.2 252,308 - GFF4884 0.44 -0.1 252,314 + GFF4884 0.44 -0.8 252,314 + GFF4884 0.44 -1.3 252,314 + GFF4884 0.44 -1.1 252,315 - GFF4884 0.45 +0.4 252,315 - GFF4884 0.45 -0.8 252,438 - GFF4884 0.60 -0.3 252,438 - GFF4884 0.60 -0.1 252,480 + GFF4884 0.65 -0.4 252,635 + GFF4884 0.83 -0.5 252,635 + GFF4884 0.83 -0.2 252,635 + GFF4884 0.83 +0.2 252,635 + GFF4884 0.83 -0.6 252,635 + GFF4884 0.83 -0.3 252,635 + GFF4884 0.83 +0.1 252,635 + GFF4884 0.83 +0.5 252,635 + GFF4884 0.83 +0.3 252,635 + GFF4884 0.83 -1.1 252,635 + GFF4884 0.83 -0.6 252,635 + GFF4884 0.83 -0.4 252,635 + GFF4884 0.83 +0.8 252,635 + GFF4884 0.83 -0.1 252,635 + GFF4884 0.83 -0.8 252,635 + GFF4884 0.83 -0.7 252,636 - GFF4884 0.84 +0.1 252,636 - GFF4884 0.84 -0.2 252,636 - GFF4884 0.84 +0.3 252,636 - GFF4884 0.84 +0.7 252,636 - GFF4884 0.84 -0.5 252,636 - GFF4884 0.84 +0.1 252,636 - GFF4884 0.84 -0.3 252,636 - GFF4884 0.84 -0.8 252,636 - GFF4884 0.84 -0.9 252,636 - GFF4884 0.84 -0.5 252,636 - GFF4884 0.84 +0.7 253,035 + GFF4883 0.46 -0.9 253,035 + GFF4883 0.46 +0.9 253,036 - GFF4883 0.46 -0.7 253,227 + GFF4883 0.77 +0.5 253,228 - GFF4883 0.77 -1.1 253,228 - GFF4883 0.77 -0.4 253,228 - GFF4883 0.77 -0.5 253,228 - GFF4883 0.77 +0.2 253,305 + GFF4883 0.89 -0.7 253,305 + GFF4883 0.89 -1.3 253,305 + GFF4883 0.89 -0.0 253,305 + GFF4883 0.89 -0.1 253,306 - GFF4883 0.89 +0.2 253,306 - GFF4883 0.89 -1.6 253,306 - GFF4883 0.89 -0.5 253,306 - GFF4883 0.89 -0.2 253,306 - GFF4883 0.89 +1.5 253,306 - GFF4883 0.89 -0.5 253,306 - GFF4883 0.89 -1.0 253,306 - GFF4883 0.89 -2.3 253,345 + -0.2 253,345 + +0.9 253,345 + -0.3 253,345 + -1.5 253,345 + +1.0 253,345 + -0.1 253,345 + +0.2 253,345 + +1.0 253,345 + +1.4 253,345 + +0.1 253,345 + -2.2 253,345 + +0.4 253,345 + -0.1 253,345 + -0.3 253,345 + +0.2 253,345 + +0.3 253,345 + +0.4 253,345 + -0.0 253,345 + -0.7 253,345 + -0.8 253,345 + +0.2 253,345 + -2.9 253,345 + -0.2 253,345 + +1.3 253,345 + -0.4 253,345 + -0.1 253,346 - -1.8 253,346 - -0.9 253,346 - -1.6 253,346 - -0.2 253,346 - -0.7 253,346 - +0.5 253,346 - -0.3 253,346 - +0.2 253,346 - +0.2 253,346 - -0.1 253,346 - +0.3 253,346 - +0.1 253,346 - -1.2 253,346 - +0.8 253,346 - -0.8 253,346 - -0.6 253,346 - -1.3 253,346 - -0.3 253,346 - -0.2 253,346 - +0.3 253,354 + -0.0 253,354 + -0.5 253,354 + +0.5 253,354 + -1.9 253,354 + +0.1 253,355 - +0.3 253,355 - +0.6 253,355 - +1.0 253,557 + -0.1 253,557 + +1.0 253,557 + +0.5 253,557 + +0.8 253,557 + -0.1 253,557 + +0.2 253,557 + +1.5 253,557 + -0.1 253,558 - +0.8 253,558 - +0.2 253,558 - +0.5 253,674 + +0.4 253,674 + -0.3 253,674 + +0.8 253,675 - +0.1 253,675 - +0.5 253,896 + GFF4886 0.63 +0.9 253,896 + GFF4886 0.63 -0.2 253,896 + GFF4886 0.63 +0.2 253,969 - GFF4886 0.87 -0.0 253,969 - GFF4886 0.87 -0.7 253,969 - GFF4886 0.87 +0.3 254,004 + -0.6 254,004 + -0.4 254,004 + -1.1 254,004 + +0.5 254,004 + -0.5 254,004 + -0.1 254,005 - +0.9 254,005 - +0.8 254,005 - -0.3 254,005 - +0.8 254,005 - -0.3 254,005 - +1.1 254,005 - -0.3 254,005 - -1.3 254,219 + GFF4887 0.25 +0.4 254,220 - GFF4887 0.25 -0.2 254,220 - GFF4887 0.25 -0.7 254,237 + GFF4887 0.32 -0.2 254,238 - GFF4887 0.32 -1.3 254,238 - GFF4887 0.32 -1.3 254,238 - GFF4887 0.32 -0.1 254,264 + GFF4887 0.41 -0.5 254,265 - GFF4887 0.42 -0.1 254,265 - GFF4887 0.42 +0.0 254,310 + GFF4887 0.58 -0.3 254,310 + GFF4887 0.58 +1.1 254,311 - GFF4887 0.58 +0.4 254,311 - GFF4887 0.58 +0.2 254,311 - GFF4887 0.58 +1.9 254,311 - GFF4887 0.58 -0.7 254,312 + GFF4887 0.58 -1.1 254,312 + GFF4887 0.58 -1.2 254,312 + GFF4887 0.58 -1.2 254,312 + GFF4887 0.58 +0.5 254,312 + GFF4887 0.58 -1.3 254,312 + GFF4887 0.58 +0.3 254,312 + GFF4887 0.58 +0.8 254,312 + GFF4887 0.58 +0.6 254,312 + GFF4887 0.58 -0.4 254,312 + GFF4887 0.58 +0.1 254,312 + GFF4887 0.58 -0.2 254,312 + GFF4887 0.58 -0.0 254,312 + GFF4887 0.58 +0.5 254,312 + GFF4887 0.58 -2.2 254,312 + GFF4887 0.58 -0.2 254,312 + GFF4887 0.58 +1.3 254,312 + GFF4887 0.58 -0.7 254,312 + GFF4887 0.58 -0.3 254,312 + GFF4887 0.58 -0.6 254,312 + GFF4887 0.58 +1.5 254,312 + GFF4887 0.58 -0.1 254,313 - GFF4887 0.59 +0.4 254,313 - GFF4887 0.59 -0.3 254,313 - GFF4887 0.59 -0.3 254,313 - GFF4887 0.59 -1.1 254,313 - GFF4887 0.59 -0.9 254,313 - GFF4887 0.59 +0.9 254,313 - GFF4887 0.59 -0.6 254,313 - GFF4887 0.59 -0.5 254,313 - GFF4887 0.59 -0.5
Or see this region's nucleotide sequence