Strain Fitness in Variovorax sp. SCN45 around GFF4675

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
32,237	+		GFF4674	0.29	-0.5
32,237	+		GFF4674	0.29	+0.4
32,237	+		GFF4674	0.29	-0.3
32,237	+		GFF4674	0.29	-1.1
32,237	+		GFF4674	0.29	-0.7
32,238	-		GFF4674	0.29	-0.1
32,783	+		GFF4674	0.70	-1.0
32,784	-		GFF4674	0.70	-0.7
32,784	-		GFF4674	0.70	+0.1
32,888	+		GFF4674	0.77	-0.5
32,888	+		GFF4674	0.77	-0.3
32,888	+		GFF4674	0.77	+1.5
32,888	+		GFF4674	0.77	+0.2
32,888	+		GFF4674	0.77	+0.1
32,888	+		GFF4674	0.77	-2.4
32,888	+		GFF4674	0.77	+0.8
32,888	+		GFF4674	0.77	+0.1
32,889	-		GFF4674	0.77	+0.3
33,011	+		GFF4674	0.87	-0.5
33,011	+		GFF4674	0.87	-0.6
33,011	+		GFF4674	0.87	-0.4
33,012	-		GFF4674	0.87	-0.8
33,012	-		GFF4674	0.87	-0.1
33,107	+				-1.1
33,107	+				+0.1
33,107	+				-0.3
33,108	-				+0.0
33,108	-				-0.1
33,108	-				-0.4
33,108	-				-0.8
33,108	-				-0.6
33,108	-				-0.3
33,108	-				+0.9
33,108	-				+0.9
33,108	-				-0.3
33,167	+				-1.9
33,167	+				-0.7
33,167	+				+0.1
33,167	+				-0.1
33,167	+				-0.8
33,167	+				-1.4
33,168	-				+0.4
33,168	-				-0.6
33,168	-				-1.5
33,168	-				-1.7
33,168	-				+1.5
33,188	+				+2.0
33,188	+				-0.3
33,188	+				-0.7
33,188	+				-0.8
33,189	-				+1.8
33,189	-				-0.1
33,297	+		GFF4675	0.18	-0.9
33,297	+		GFF4675	0.18	-0.9
33,297	+		GFF4675	0.18	+0.8
33,297	+		GFF4675	0.18	-2.7
33,297	+		GFF4675	0.18	-2.4
33,297	+		GFF4675	0.18	-0.2
33,297	+		GFF4675	0.18	-0.6
33,297	+		GFF4675	0.18	+0.9
33,297	+		GFF4675	0.18	-0.5
33,298	-		GFF4675	0.19	-0.6
33,298	-		GFF4675	0.19	-0.7
33,298	-		GFF4675	0.19	-0.6
33,313	+		GFF4675	0.21	-0.6
33,313	+		GFF4675	0.21	-0.7
33,314	-		GFF4675	0.21	-1.2
33,404	+		GFF4675	0.37	+0.4
33,404	+		GFF4675	0.37	-1.3
33,405	-		GFF4675	0.38	-0.7
33,405	-		GFF4675	0.38	+0.1
33,552	+		GFF4675	0.64	+0.0
33,553	-		GFF4675	0.64	-0.3
33,575	-		GFF4675	0.68	-0.1
33,638	+		GFF4675	0.79	-0.3
33,639	-		GFF4675	0.79	-0.9
33,643	+		GFF4675	0.80	+0.0
33,643	+		GFF4675	0.80	-1.1
33,643	+		GFF4675	0.80	-0.5
33,767	+				-1.4
33,767	+				+0.5
33,767	+				-0.6
33,767	+				-1.5
33,767	+				-0.2
33,767	+				+0.2
33,767	+				+0.2
33,767	+				-0.3
33,767	+				-0.5
33,767	+				+0.8
33,767	+				-0.2
33,767	+				-0.1
33,767	+				-0.5
33,767	+				-0.2
33,767	+				-0.3
33,767	+				+0.2
33,767	+				-0.5
33,767	+				-0.9
33,767	+				-2.0
33,768	-				-2.3
33,768	-				+0.5
33,768	-				+2.8
33,768	-				-0.2
33,768	-				-0.2
33,768	-				-0.4
33,768	-				+1.1
33,768	-				-0.1
33,768	-				-0.1
33,768	-				-0.5
33,768	-				-1.3
33,828	+				+0.8
33,828	+				-0.6
33,829	-				+0.5
33,829	-				-0.5
33,933	+		GFF4676	0.15	-1.3
33,933	+		GFF4676	0.15	+0.2
33,933	+		GFF4676	0.15	-0.2
33,933	+		GFF4676	0.15	-0.1
33,933	+		GFF4676	0.15	+0.2
33,933	+		GFF4676	0.15	-0.5
33,934	-		GFF4676	0.15	-0.8
33,934	-		GFF4676	0.15	-0.8
33,934	-		GFF4676	0.15	+0.3
33,934	-		GFF4676	0.15	+0.1
33,934	-		GFF4676	0.15	-0.1
33,934	-		GFF4676	0.15	-0.6
33,934	-		GFF4676	0.15	-0.1
34,040	+		GFF4676	0.24	-0.3
34,040	+		GFF4676	0.24	+0.5
34,041	-		GFF4676	0.24	-0.5
34,041	-		GFF4676	0.24	-0.1
34,041	-		GFF4676	0.24	+0.4
34,151	+		GFF4676	0.34	+0.5
34,151	+		GFF4676	0.34	+0.0
34,152	-		GFF4676	0.34	-0.5
34,152	-		GFF4676	0.34	-0.9
34,152	-		GFF4676	0.34	-0.8
34,226	+		GFF4676	0.40	+0.7
34,227	-		GFF4676	0.41	-0.7
34,331	+		GFF4676	0.50	+0.5
34,331	+		GFF4676	0.50	+1.4
34,331	+		GFF4676	0.50	-0.1
34,331	+		GFF4676	0.50	-0.1
34,331	+		GFF4676	0.50	+0.4
34,331	+		GFF4676	0.50	-0.2
34,331	+		GFF4676	0.50	+0.0
34,331	+		GFF4676	0.50	-0.3
34,331	+		GFF4676	0.50	-0.4
34,331	+		GFF4676	0.50	+0.1
34,331	+		GFF4676	0.50	-0.7
34,331	+		GFF4676	0.50	-0.2
34,332	-		GFF4676	0.50	-1.2
34,332	-		GFF4676	0.50	+2.1
34,332	-		GFF4676	0.50	-1.5
34,332	-		GFF4676	0.50	-0.4
34,332	-		GFF4676	0.50	-0.5
34,332	-		GFF4676	0.50	-0.6
34,523	+		GFF4676	0.67	+0.3
34,524	-		GFF4676	0.67	-0.7
34,524	-		GFF4676	0.67	+0.4
34,524	-		GFF4676	0.67	-0.4
34,524	-		GFF4676	0.67	+0.1
34,524	-		GFF4676	0.67	+1.1
34,524	-		GFF4676	0.67	-0.5
34,524	-		GFF4676	0.67	-0.5
34,526	+		GFF4676	0.67	-0.5
34,526	+		GFF4676	0.67	+0.8
34,527	-		GFF4676	0.67	-0.7
34,527	-		GFF4676	0.67	-0.3
34,627	+		GFF4676	0.76	-2.1
34,627	+		GFF4676	0.76	-0.9
34,627	+		GFF4676	0.76	+0.2
34,627	+		GFF4676	0.76	-1.0
34,627	+		GFF4676	0.76	-0.2
34,628	-		GFF4676	0.76	-0.6
34,628	-		GFF4676	0.76	-0.3
34,628	-		GFF4676	0.76	-0.9
34,628	-		GFF4676	0.76	-0.4
34,694	+		GFF4676	0.82	+0.6
34,694	+		GFF4676	0.82	-0.3
34,694	+		GFF4676	0.82	+0.5
34,694	+		GFF4676	0.82	-0.2
34,694	+		GFF4676	0.82	+0.7
34,694	+		GFF4676	0.82	-0.3
34,694	+		GFF4676	0.82	-0.6
34,694	+		GFF4676	0.82	+0.0
34,694	+		GFF4676	0.82	-1.1
34,695	-		GFF4676	0.82	-1.2
34,695	-		GFF4676	0.82	-0.4
34,695	-		GFF4676	0.82	-0.9
34,695	-		GFF4676	0.82	-0.1
34,695	-		GFF4676	0.82	-0.5
34,695	-		GFF4676	0.82	+1.3
34,695	-		GFF4676	0.82	-2.8
34,697	+		GFF4676	0.82	-0.3
34,697	+		GFF4676	0.82	+0.5
34,697	+		GFF4676	0.82	+0.3
34,697	+		GFF4676	0.82	+0.5
34,697	+		GFF4676	0.82	-0.0
34,698	-		GFF4676	0.82	-0.8
34,698	-		GFF4676	0.82	+0.4
34,698	-		GFF4676	0.82	+0.5
34,698	-		GFF4676	0.82	+0.5
34,698	-		GFF4676	0.82	-2.7
34,698	-		GFF4676	0.82	-0.7
34,698	-		GFF4676	0.82	+0.3
34,698	-		GFF4676	0.82	-0.9
34,698	-		GFF4676	0.82	+0.4
34,698	-		GFF4676	0.82	+0.0
34,728	-		GFF4676	0.85	+0.5
34,728	-		GFF4676	0.85	-0.5
34,728	-		GFF4676	0.85	-0.5