Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4366 and GFF4367 are separated by 7 nucleotides GFF4367 and GFF4368 are separated by 35 nucleotides GFF4368 and GFF4369 are separated by 51 nucleotides GFF4369 and GFF4370 are separated by 110 nucleotides
GFF4366 - BUG/TctC family periplasmic protein, at 289,791 to 290,777
GFF4366
GFF4367 - KEGG: regulator of ribonuclease activity A, at 290,785 to 291,270
GFF4367
GFF4368 - Peptidoglycan D,D-transpeptidase MrdA (EC 3.4.16.4), at 291,306 to 293,294
GFF4368
GFF4369 - no description, at 293,346 to 293,600
GFF4369
GFF4370 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 293,711 to 296,095
GFF4370
Position (kb) 291
292
293
294 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 290.498 kb on - strand, within GFF4366 at 290.541 kb on + strand, within GFF4366 at 291.288 kb on - strand at 292.135 kb on + strand, within GFF4368 at 292.136 kb on - strand, within GFF4368 at 292.136 kb on - strand, within GFF4368 at 292.136 kb on - strand, within GFF4368 at 292.226 kb on - strand, within GFF4368 at 292.304 kb on - strand, within GFF4368 at 292.304 kb on - strand, within GFF4368 at 292.322 kb on - strand, within GFF4368 at 292.702 kb on + strand, within GFF4368 at 292.771 kb on + strand, within GFF4368 at 293.047 kb on + strand, within GFF4368 at 293.048 kb on - strand, within GFF4368 at 293.056 kb on + strand, within GFF4368 at 293.328 kb on + strand at 293.451 kb on + strand, within GFF4369 at 293.476 kb on + strand, within GFF4369 at 293.476 kb on + strand, within GFF4369 at 293.477 kb on - strand, within GFF4369 at 293.505 kb on - strand, within GFF4369 at 293.505 kb on - strand, within GFF4369 at 293.515 kb on + strand, within GFF4369 at 293.516 kb on - strand, within GFF4369 at 293.705 kb on - strand at 293.793 kb on - strand at 293.996 kb on + strand, within GFF4370 at 293.996 kb on + strand, within GFF4370 at 293.996 kb on - strand, within GFF4370 at 293.997 kb on - strand, within GFF4370
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 290,498 - GFF4366 0.72 -0.7 290,541 + GFF4366 0.76 +0.9 291,288 - -0.6 292,135 + GFF4368 0.42 -0.2 292,136 - GFF4368 0.42 -0.9 292,136 - GFF4368 0.42 +0.3 292,136 - GFF4368 0.42 -0.0 292,226 - GFF4368 0.46 -1.6 292,304 - GFF4368 0.50 -0.7 292,304 - GFF4368 0.50 -0.7 292,322 - GFF4368 0.51 -0.2 292,702 + GFF4368 0.70 -0.5 292,771 + GFF4368 0.74 -0.7 293,047 + GFF4368 0.88 +0.9 293,048 - GFF4368 0.88 -0.9 293,056 + GFF4368 0.88 -0.4 293,328 + +0.1 293,451 + GFF4369 0.41 -0.8 293,476 + GFF4369 0.51 -2.9 293,476 + GFF4369 0.51 +0.9 293,477 - GFF4369 0.51 +2.3 293,505 - GFF4369 0.62 -0.5 293,505 - GFF4369 0.62 +1.1 293,515 + GFF4369 0.66 +0.3 293,516 - GFF4369 0.67 +0.7 293,705 - -0.3 293,793 - +0.2 293,996 + GFF4370 0.12 -1.2 293,996 + GFF4370 0.12 +0.5 293,996 - GFF4370 0.12 -1.0 293,997 - GFF4370 0.12 +0.0
Or see this region's nucleotide sequence