Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4349 and GFF4350 overlap by 4 nucleotides GFF4350 and GFF4351 are separated by 18 nucleotides GFF4351 and GFF4352 are separated by 22 nucleotides
GFF4349 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 272,088 to 272,786
GFF4349
GFF4350 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 272,783 to 273,478
GFF4350
GFF4351 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 273,497 to 274,678
GFF4351
GFF4352 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 274,701 to 276,257
GFF4352
Position (kb) 273
274
275 Strain fitness (log2 ratio) -2
-1
0
1 at 272.939 kb on - strand, within GFF4350 at 273.692 kb on + strand, within GFF4351 at 273.805 kb on + strand, within GFF4351 at 274.118 kb on + strand, within GFF4351 at 274.119 kb on - strand, within GFF4351 at 274.119 kb on - strand, within GFF4351 at 274.163 kb on + strand, within GFF4351 at 274.163 kb on + strand, within GFF4351 at 274.163 kb on + strand, within GFF4351 at 274.164 kb on - strand, within GFF4351 at 274.164 kb on - strand, within GFF4351 at 274.166 kb on + strand, within GFF4351 at 274.229 kb on + strand, within GFF4351 at 274.320 kb on - strand, within GFF4351 at 274.397 kb on + strand, within GFF4351 at 274.415 kb on + strand, within GFF4351 at 274.416 kb on - strand, within GFF4351 at 274.416 kb on - strand, within GFF4351 at 274.416 kb on - strand, within GFF4351 at 274.677 kb on - strand at 274.819 kb on - strand at 275.205 kb on + strand, within GFF4352 at 275.206 kb on - strand, within GFF4352 at 275.503 kb on - strand, within GFF4352
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 272,939 - GFF4350 0.22 +1.0 273,692 + GFF4351 0.16 -1.6 273,805 + GFF4351 0.26 -1.1 274,118 + GFF4351 0.53 -0.1 274,119 - GFF4351 0.53 +0.4 274,119 - GFF4351 0.53 -0.1 274,163 + GFF4351 0.56 +1.3 274,163 + GFF4351 0.56 +1.1 274,163 + GFF4351 0.56 -0.2 274,164 - GFF4351 0.56 -0.7 274,164 - GFF4351 0.56 -0.1 274,166 + GFF4351 0.57 -0.9 274,229 + GFF4351 0.62 +0.6 274,320 - GFF4351 0.70 +0.6 274,397 + GFF4351 0.76 +0.3 274,415 + GFF4351 0.78 +0.2 274,416 - GFF4351 0.78 -0.4 274,416 - GFF4351 0.78 -1.1 274,416 - GFF4351 0.78 +0.4 274,677 - +0.3 274,819 - +0.0 275,205 + GFF4352 0.32 -2.0 275,206 - GFF4352 0.32 -0.1 275,503 - GFF4352 0.52 +0.3
Or see this region's nucleotide sequence