Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3888 and GFF3889 are separated by 36 nucleotides GFF3889 and GFF3890 are separated by 16 nucleotides GFF3890 and GFF3891 are separated by 49 nucleotides GFF3891 and GFF3892 overlap by 4 nucleotides
GFF3888 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 87,033 to 88,121
GFF3888
GFF3889 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 88,158 to 88,814
GFF3889
GFF3890 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 88,831 to 90,252
GFF3890
GFF3891 - Transcriptional regulator, LysR family, at 90,302 to 91,246
GFF3891
GFF3892 - no description, at 91,243 to 91,359
GFF3892
Position (kb)
88
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 87.904 kb on + strand, within GFF3888 at 87.905 kb on - strand, within GFF3888 at 87.905 kb on - strand, within GFF3888 at 88.178 kb on + strand at 88.178 kb on + strand at 88.178 kb on + strand at 88.178 kb on + strand at 88.178 kb on + strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.179 kb on - strand at 88.285 kb on + strand, within GFF3889 at 88.285 kb on + strand, within GFF3889 at 88.285 kb on + strand, within GFF3889 at 88.286 kb on - strand, within GFF3889 at 88.286 kb on - strand, within GFF3889 at 88.481 kb on - strand, within GFF3889 at 88.594 kb on + strand, within GFF3889 at 88.595 kb on - strand, within GFF3889 at 88.719 kb on - strand, within GFF3889 at 89.267 kb on + strand, within GFF3890 at 89.268 kb on - strand, within GFF3890 at 89.459 kb on + strand, within GFF3890 at 89.460 kb on - strand, within GFF3890 at 89.516 kb on + strand, within GFF3890 at 89.516 kb on + strand, within GFF3890 at 89.517 kb on - strand, within GFF3890 at 89.517 kb on - strand, within GFF3890 at 89.645 kb on + strand, within GFF3890 at 89.646 kb on - strand, within GFF3890 at 89.646 kb on - strand, within GFF3890 at 89.646 kb on - strand, within GFF3890 at 89.646 kb on - strand, within GFF3890 at 90.032 kb on + strand, within GFF3890 at 90.090 kb on + strand, within GFF3890 at 90.091 kb on - strand, within GFF3890 at 90.176 kb on + strand at 90.177 kb on - strand at 90.236 kb on + strand at 90.237 kb on - strand at 90.304 kb on - strand at 90.304 kb on - strand at 90.587 kb on - strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.612 kb on + strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.613 kb on - strand, within GFF3891 at 90.960 kb on + strand, within GFF3891 at 90.961 kb on - strand, within GFF3891 at 90.961 kb on - strand, within GFF3891 at 90.961 kb on - strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.996 kb on + strand, within GFF3891 at 90.997 kb on - strand, within GFF3891 at 90.997 kb on - strand, within GFF3891 at 90.997 kb on - strand, within GFF3891 at 90.997 kb on - strand, within GFF3891 at 90.997 kb on - strand, within GFF3891 at 91.068 kb on + strand, within GFF3891
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 87,904 + GFF3888 0.80 -0.9 87,905 - GFF3888 0.80 -2.2 87,905 - GFF3888 0.80 -0.9 88,178 + -0.6 88,178 + -0.9 88,178 + -0.1 88,178 + +0.7 88,178 + -0.4 88,179 - +0.2 88,179 - -1.7 88,179 - -0.2 88,179 - +0.2 88,179 - +1.9 88,179 - +0.4 88,179 - -0.2 88,179 - +0.1 88,179 - -0.4 88,285 + GFF3889 0.19 -2.4 88,285 + GFF3889 0.19 -0.4 88,285 + GFF3889 0.19 -0.3 88,286 - GFF3889 0.19 +0.1 88,286 - GFF3889 0.19 -0.9 88,481 - GFF3889 0.49 -1.7 88,594 + GFF3889 0.66 -1.6 88,595 - GFF3889 0.67 -0.9 88,719 - GFF3889 0.85 -1.4 89,267 + GFF3890 0.31 -0.6 89,268 - GFF3890 0.31 -1.4 89,459 + GFF3890 0.44 -1.4 89,460 - GFF3890 0.44 -1.4 89,516 + GFF3890 0.48 -1.7 89,516 + GFF3890 0.48 -2.0 89,517 - GFF3890 0.48 -2.1 89,517 - GFF3890 0.48 -1.3 89,645 + GFF3890 0.57 -0.6 89,646 - GFF3890 0.57 -1.7 89,646 - GFF3890 0.57 -2.5 89,646 - GFF3890 0.57 -1.3 89,646 - GFF3890 0.57 -1.6 90,032 + GFF3890 0.84 -1.1 90,090 + GFF3890 0.89 -1.9 90,091 - GFF3890 0.89 +0.1 90,176 + -0.6 90,177 - -0.9 90,236 + -0.9 90,237 - -0.3 90,304 - +0.5 90,304 - -0.9 90,587 - GFF3891 0.30 +0.0 90,612 + GFF3891 0.33 -0.2 90,612 + GFF3891 0.33 -0.4 90,612 + GFF3891 0.33 -0.5 90,612 + GFF3891 0.33 -0.3 90,612 + GFF3891 0.33 -0.7 90,612 + GFF3891 0.33 -0.4 90,612 + GFF3891 0.33 +0.3 90,612 + GFF3891 0.33 +0.3 90,613 - GFF3891 0.33 +0.1 90,613 - GFF3891 0.33 -0.0 90,613 - GFF3891 0.33 -1.3 90,613 - GFF3891 0.33 +0.1 90,613 - GFF3891 0.33 -0.3 90,613 - GFF3891 0.33 +0.1 90,613 - GFF3891 0.33 +0.3 90,613 - GFF3891 0.33 -0.1 90,960 + GFF3891 0.70 -0.4 90,961 - GFF3891 0.70 -0.9 90,961 - GFF3891 0.70 -0.9 90,961 - GFF3891 0.70 -0.1 90,996 + GFF3891 0.73 -0.2 90,996 + GFF3891 0.73 +0.3 90,996 + GFF3891 0.73 -0.8 90,996 + GFF3891 0.73 -0.3 90,996 + GFF3891 0.73 -1.1 90,996 + GFF3891 0.73 -0.0 90,996 + GFF3891 0.73 -0.7 90,996 + GFF3891 0.73 -0.1 90,997 - GFF3891 0.74 +0.6 90,997 - GFF3891 0.74 +0.1 90,997 - GFF3891 0.74 -1.8 90,997 - GFF3891 0.74 -0.3 90,997 - GFF3891 0.74 -0.8 91,068 + GFF3891 0.81 -0.6
Or see this region's nucleotide sequence