Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3730 and GFF3731 are separated by 76 nucleotides GFF3731 and GFF3732 are separated by 11 nucleotides GFF3732 and GFF3733 are separated by 98 nucleotides GFF3733 and GFF3734 overlap by 4 nucleotides
GFF3730 - Integral membrane protein, at 228,472 to 229,290
GFF3730
GFF3731 - Glutathione S-transferase, at 229,367 to 230,095
GFF3731
GFF3732 - hypothetical protein, at 230,107 to 230,604
GFF3732
GFF3733 - no description, at 230,703 to 231,599
GFF3733
GFF3734 - RNA:NAD 2'-phosphotransferase, at 231,596 to 232,132
GFF3734
Position (kb) 230
231 Strain fitness (log2 ratio) -2
-1
0
1
2 at 229.244 kb on - strand at 229.373 kb on + strand at 229.373 kb on + strand at 229.385 kb on + strand at 229.385 kb on + strand at 229.451 kb on + strand, within GFF3731 at 229.451 kb on + strand, within GFF3731 at 229.451 kb on + strand, within GFF3731 at 229.452 kb on - strand, within GFF3731 at 229.607 kb on + strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.742 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 230.021 kb on + strand, within GFF3731 at 230.075 kb on + strand at 230.075 kb on + strand at 230.075 kb on + strand at 230.094 kb on - strand at 230.195 kb on + strand, within GFF3732 at 230.195 kb on + strand, within GFF3732 at 230.641 kb on + strand at 230.641 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.667 kb on + strand at 230.700 kb on + strand at 230.859 kb on + strand, within GFF3733 at 230.859 kb on + strand, within GFF3733 at 231.015 kb on + strand, within GFF3733 at 231.015 kb on + strand, within GFF3733 at 231.016 kb on - strand, within GFF3733 at 231.021 kb on + strand, within GFF3733 at 231.021 kb on + strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.022 kb on - strand, within GFF3733 at 231.191 kb on + strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.192 kb on - strand, within GFF3733 at 231.224 kb on + strand, within GFF3733 at 231.498 kb on + strand, within GFF3733 at 231.499 kb on - strand, within GFF3733 at 231.499 kb on - strand, within GFF3733
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 229,244 - +0.4 229,373 + -1.8 229,373 + -0.1 229,385 + -0.0 229,385 + -0.2 229,451 + GFF3731 0.12 +1.0 229,451 + GFF3731 0.12 -0.1 229,451 + GFF3731 0.12 +0.8 229,452 - GFF3731 0.12 -1.2 229,607 + GFF3731 0.33 +2.0 229,608 - GFF3731 0.33 -0.0 229,608 - GFF3731 0.33 -0.4 229,608 - GFF3731 0.33 -0.5 229,608 - GFF3731 0.33 -1.4 229,742 + GFF3731 0.51 -0.8 229,883 + GFF3731 0.71 -1.0 229,883 + GFF3731 0.71 -1.1 229,883 + GFF3731 0.71 -0.4 229,883 + GFF3731 0.71 +1.1 229,883 + GFF3731 0.71 +0.3 229,883 + GFF3731 0.71 -0.3 229,883 + GFF3731 0.71 -0.8 229,884 - GFF3731 0.71 -0.1 229,884 - GFF3731 0.71 -0.2 229,884 - GFF3731 0.71 +0.3 229,884 - GFF3731 0.71 -0.2 230,021 + GFF3731 0.90 -0.1 230,075 + +0.6 230,075 + -0.3 230,075 + -0.7 230,094 - +0.6 230,195 + GFF3732 0.18 +0.1 230,195 + GFF3732 0.18 +1.4 230,641 + +0.1 230,641 + +0.6 230,667 + -0.5 230,667 + +0.6 230,667 + -1.0 230,667 + +0.5 230,700 + -0.2 230,859 + GFF3733 0.17 -0.8 230,859 + GFF3733 0.17 -0.2 231,015 + GFF3733 0.35 +0.2 231,015 + GFF3733 0.35 -0.2 231,016 - GFF3733 0.35 -0.5 231,021 + GFF3733 0.35 -0.2 231,021 + GFF3733 0.35 -0.2 231,022 - GFF3733 0.36 -0.6 231,022 - GFF3733 0.36 -0.6 231,022 - GFF3733 0.36 +0.5 231,022 - GFF3733 0.36 -1.4 231,022 - GFF3733 0.36 +1.6 231,022 - GFF3733 0.36 -0.5 231,191 + GFF3733 0.54 -1.3 231,192 - GFF3733 0.55 +0.2 231,192 - GFF3733 0.55 +0.6 231,192 - GFF3733 0.55 -0.4 231,192 - GFF3733 0.55 -0.5 231,224 + GFF3733 0.58 +0.5 231,498 + GFF3733 0.89 +0.4 231,499 - GFF3733 0.89 -1.1 231,499 - GFF3733 0.89 +0.1
Or see this region's nucleotide sequence