Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3728 and GFF3729 are separated by 27 nucleotides GFF3729 and GFF3730 are separated by 119 nucleotides GFF3730 and GFF3731 are separated by 76 nucleotides GFF3731 and GFF3732 are separated by 11 nucleotides
GFF3728 - no description, at 227,330 to 227,557
GFF3728
GFF3729 - Transcriptional regulator, AraC family, at 227,585 to 228,352
GFF3729
GFF3730 - Integral membrane protein, at 228,472 to 229,290
GFF3730
GFF3731 - Glutathione S-transferase, at 229,367 to 230,095
GFF3731
GFF3732 - hypothetical protein, at 230,107 to 230,604
GFF3732
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 227.661 kb on + strand at 227.661 kb on + strand at 227.661 kb on + strand at 227.991 kb on + strand, within GFF3729 at 227.992 kb on - strand, within GFF3729 at 227.992 kb on - strand, within GFF3729 at 227.992 kb on - strand, within GFF3729 at 228.099 kb on + strand, within GFF3729 at 228.099 kb on + strand, within GFF3729 at 228.231 kb on + strand, within GFF3729 at 228.231 kb on + strand, within GFF3729 at 228.296 kb on + strand at 228.296 kb on + strand at 228.297 kb on - strand at 228.393 kb on + strand at 228.393 kb on + strand at 228.433 kb on + strand at 228.433 kb on + strand at 228.433 kb on + strand at 228.433 kb on + strand at 228.433 kb on + strand at 228.433 kb on + strand at 228.434 kb on - strand at 228.434 kb on - strand at 228.434 kb on - strand at 228.434 kb on - strand at 228.434 kb on - strand at 228.434 kb on - strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.481 kb on + strand at 228.482 kb on - strand at 228.482 kb on - strand at 228.482 kb on - strand at 228.482 kb on - strand at 228.482 kb on - strand at 228.482 kb on - strand at 228.823 kb on + strand, within GFF3730 at 228.824 kb on - strand, within GFF3730 at 229.081 kb on + strand, within GFF3730 at 229.081 kb on + strand, within GFF3730 at 229.081 kb on + strand, within GFF3730 at 229.081 kb on + strand, within GFF3730 at 229.081 kb on + strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.082 kb on - strand, within GFF3730 at 229.244 kb on - strand at 229.373 kb on + strand at 229.373 kb on + strand at 229.385 kb on + strand at 229.385 kb on + strand at 229.451 kb on + strand, within GFF3731 at 229.451 kb on + strand, within GFF3731 at 229.451 kb on + strand, within GFF3731 at 229.452 kb on - strand, within GFF3731 at 229.607 kb on + strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.608 kb on - strand, within GFF3731 at 229.742 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.883 kb on + strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 229.884 kb on - strand, within GFF3731 at 230.021 kb on + strand, within GFF3731 at 230.075 kb on + strand at 230.075 kb on + strand at 230.075 kb on + strand at 230.094 kb on - strand at 230.195 kb on + strand, within GFF3732 at 230.195 kb on + strand, within GFF3732
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 227,661 + -0.6 227,661 + +1.0 227,661 + +0.2 227,991 + GFF3729 0.53 +1.0 227,992 - GFF3729 0.53 +3.0 227,992 - GFF3729 0.53 -0.6 227,992 - GFF3729 0.53 -0.6 228,099 + GFF3729 0.67 +0.5 228,099 + GFF3729 0.67 -1.6 228,231 + GFF3729 0.84 -0.1 228,231 + GFF3729 0.84 -0.4 228,296 + +0.6 228,296 + -0.1 228,297 - -1.2 228,393 + -2.8 228,393 + +1.0 228,433 + -0.0 228,433 + -0.3 228,433 + -0.8 228,433 + +0.3 228,433 + -0.4 228,433 + -0.0 228,434 - -0.3 228,434 - -0.4 228,434 - -0.4 228,434 - +0.5 228,434 - -0.4 228,434 - -1.9 228,481 + -1.1 228,481 + -1.4 228,481 + +0.4 228,481 + -0.9 228,481 + +0.2 228,481 + +0.4 228,481 + -1.0 228,481 + -0.1 228,482 - +0.1 228,482 - -0.6 228,482 - +0.2 228,482 - -2.0 228,482 - -0.1 228,482 - -0.8 228,823 + GFF3730 0.43 -0.9 228,824 - GFF3730 0.43 -0.6 229,081 + GFF3730 0.74 -0.7 229,081 + GFF3730 0.74 -0.5 229,081 + GFF3730 0.74 -0.0 229,081 + GFF3730 0.74 -0.2 229,081 + GFF3730 0.74 -0.8 229,082 - GFF3730 0.74 +0.1 229,082 - GFF3730 0.74 -0.8 229,082 - GFF3730 0.74 +1.2 229,082 - GFF3730 0.74 -0.5 229,082 - GFF3730 0.74 -1.6 229,082 - GFF3730 0.74 -1.3 229,082 - GFF3730 0.74 -1.5 229,082 - GFF3730 0.74 +0.6 229,082 - GFF3730 0.74 +0.3 229,082 - GFF3730 0.74 +1.3 229,244 - +0.4 229,373 + -1.8 229,373 + -0.1 229,385 + -0.0 229,385 + -0.2 229,451 + GFF3731 0.12 +1.0 229,451 + GFF3731 0.12 -0.1 229,451 + GFF3731 0.12 +0.8 229,452 - GFF3731 0.12 -1.2 229,607 + GFF3731 0.33 +2.0 229,608 - GFF3731 0.33 -0.0 229,608 - GFF3731 0.33 -0.4 229,608 - GFF3731 0.33 -0.5 229,608 - GFF3731 0.33 -1.4 229,742 + GFF3731 0.51 -0.8 229,883 + GFF3731 0.71 -1.0 229,883 + GFF3731 0.71 -1.1 229,883 + GFF3731 0.71 -0.4 229,883 + GFF3731 0.71 +1.1 229,883 + GFF3731 0.71 +0.3 229,883 + GFF3731 0.71 -0.3 229,883 + GFF3731 0.71 -0.8 229,884 - GFF3731 0.71 -0.1 229,884 - GFF3731 0.71 -0.2 229,884 - GFF3731 0.71 +0.3 229,884 - GFF3731 0.71 -0.2 230,021 + GFF3731 0.90 -0.1 230,075 + +0.6 230,075 + -0.3 230,075 + -0.7 230,094 - +0.6 230,195 + GFF3732 0.18 +0.1 230,195 + GFF3732 0.18 +1.4
Or see this region's nucleotide sequence