Strain Fitness in Variovorax sp. SCN45 around GFF3478

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3477 and GFF3478 overlap by 4 nucleotidesGFF3478 and GFF3479 are separated by 103 nucleotides GFF3477 - Phosphoprotein phosphatase, at 298,842 to 300,410 GFF3477 GFF3478 - Cytochrome c peroxidase (EC 1.11.1.5), at 300,407 to 301,759 GFF3478 GFF3479 - no description, at 301,863 to 303,020 GFF3479 Position (kb) 300 301 302Strain fitness (log2 ratio) -2 -1 0 1 2at 299.524 kb on - strand, within GFF3477at 299.529 kb on + strand, within GFF3477at 299.731 kb on - strand, within GFF3477at 299.731 kb on - strand, within GFF3477at 299.731 kb on - strand, within GFF3477at 299.781 kb on + strand, within GFF3477at 299.781 kb on + strand, within GFF3477at 299.782 kb on - strand, within GFF3477at 299.782 kb on - strand, within GFF3477at 299.782 kb on - strand, within GFF3477at 299.782 kb on - strand, within GFF3477at 300.060 kb on + strand, within GFF3477at 300.060 kb on + strand, within GFF3477at 300.060 kb on + strand, within GFF3477at 300.061 kb on - strand, within GFF3477at 300.061 kb on - strand, within GFF3477at 300.064 kb on - strand, within GFF3477at 300.321 kb on + strandat 300.321 kb on + strandat 300.321 kb on + strandat 300.321 kb on + strandat 300.322 kb on - strandat 300.322 kb on - strandat 300.322 kb on - strandat 300.833 kb on + strand, within GFF3478at 300.833 kb on + strand, within GFF3478at 300.833 kb on + strand, within GFF3478at 300.833 kb on + strand, within GFF3478at 300.833 kb on + strand, within GFF3478at 300.834 kb on - strand, within GFF3478at 300.834 kb on - strand, within GFF3478at 300.842 kb on + strand, within GFF3478at 300.843 kb on - strand, within GFF3478at 300.863 kb on + strand, within GFF3478at 300.864 kb on - strand, within GFF3478at 300.864 kb on - strand, within GFF3478at 300.864 kb on - strand, within GFF3478at 301.115 kb on + strand, within GFF3478at 301.115 kb on + strand, within GFF3478at 301.115 kb on + strand, within GFF3478at 301.116 kb on - strand, within GFF3478at 301.116 kb on - strand, within GFF3478at 301.116 kb on - strand, within GFF3478at 301.163 kb on + strand, within GFF3478at 301.164 kb on - strand, within GFF3478at 301.164 kb on - strand, within GFF3478at 301.311 kb on - strand, within GFF3478at 301.436 kb on + strand, within GFF3478at 301.436 kb on + strand, within GFF3478at 301.436 kb on + strand, within GFF3478at 301.529 kb on + strand, within GFF3478at 301.529 kb on + strand, within GFF3478at 301.529 kb on + strand, within GFF3478at 301.529 kb on + strand, within GFF3478at 301.530 kb on - strand, within GFF3478at 301.530 kb on - strand, within GFF3478at 301.562 kb on + strand, within GFF3478at 301.563 kb on - strand, within GFF3478at 301.563 kb on - strand, within GFF3478at 301.601 kb on + strand, within GFF3478at 301.601 kb on + strand, within GFF3478at 301.602 kb on - strand, within GFF3478at 301.602 kb on - strand, within GFF3478at 301.602 kb on - strand, within GFF3478at 301.602 kb on - strand, within GFF3478at 301.724 kb on + strandat 301.724 kb on + strandat 301.724 kb on + strandat 301.724 kb on + strandat 301.725 kb on - strandat 301.725 kb on - strandat 301.725 kb on - strandat 301.725 kb on - strandat 301.725 kb on - strandat 301.725 kb on - strandat 301.757 kb on + strandat 301.758 kb on - strandat 301.758 kb on - strandat 301.803 kb on + strandat 301.803 kb on + strandat 301.803 kb on + strandat 302.049 kb on + strand, within GFF3479at 302.049 kb on + strand, within GFF3479at 302.050 kb on - strand, within GFF3479at 302.050 kb on - strand, within GFF3479at 302.340 kb on + strand, within GFF3479at 302.341 kb on - strand, within GFF3479at 302.341 kb on - strand, within GFF3479at 302.341 kb on - strand, within GFF3479at 302.643 kb on + strand, within GFF3479at 302.644 kb on - strand, within GFF3479at 302.742 kb on + strand, within GFF3479at 302.742 kb on + strand, within GFF3479at 302.743 kb on - strand, within GFF3479at 302.743 kb on - strand, within GFF3479at 302.743 kb on - strand, within GFF3479at 302.743 kb on - strand, within GFF3479at 302.743 kb on - strand, within GFF3479

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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299,524 - GFF3477 0.43 -1.9
299,529 + GFF3477 0.44 +0.4
299,731 - GFF3477 0.57 -0.2
299,731 - GFF3477 0.57 +1.7
299,731 - GFF3477 0.57 +0.4
299,781 + GFF3477 0.60 +0.2
299,781 + GFF3477 0.60 -0.6
299,782 - GFF3477 0.60 -0.6
299,782 - GFF3477 0.60 -0.3
299,782 - GFF3477 0.60 -0.0
299,782 - GFF3477 0.60 -0.9
300,060 + GFF3477 0.78 +0.1
300,060 + GFF3477 0.78 -1.1
300,060 + GFF3477 0.78 -0.9
300,061 - GFF3477 0.78 -0.4
300,061 - GFF3477 0.78 -0.2
300,064 - GFF3477 0.78 -0.5
300,321 + -0.5
300,321 + -1.5
300,321 + -0.4
300,321 + -0.5
300,322 - -0.0
300,322 - -0.8
300,322 - +0.4
300,833 + GFF3478 0.31 -0.2
300,833 + GFF3478 0.31 +0.1
300,833 + GFF3478 0.31 -1.2
300,833 + GFF3478 0.31 -0.4
300,833 + GFF3478 0.31 -0.3
300,834 - GFF3478 0.32 -0.0
300,834 - GFF3478 0.32 -1.5
300,842 + GFF3478 0.32 +0.3
300,843 - GFF3478 0.32 +0.1
300,863 + GFF3478 0.34 +0.8
300,864 - GFF3478 0.34 +0.4
300,864 - GFF3478 0.34 +0.2
300,864 - GFF3478 0.34 -0.1
301,115 + GFF3478 0.52 -0.2
301,115 + GFF3478 0.52 +0.2
301,115 + GFF3478 0.52 +0.2
301,116 - GFF3478 0.52 -0.7
301,116 - GFF3478 0.52 +0.2
301,116 - GFF3478 0.52 -0.4
301,163 + GFF3478 0.56 -0.3
301,164 - GFF3478 0.56 +0.0
301,164 - GFF3478 0.56 -1.5
301,311 - GFF3478 0.67 -0.7
301,436 + GFF3478 0.76 +0.1
301,436 + GFF3478 0.76 +0.5
301,436 + GFF3478 0.76 +0.3
301,529 + GFF3478 0.83 +1.1
301,529 + GFF3478 0.83 -0.5
301,529 + GFF3478 0.83 +0.5
301,529 + GFF3478 0.83 +0.1
301,530 - GFF3478 0.83 +1.8
301,530 - GFF3478 0.83 +1.1
301,562 + GFF3478 0.85 +0.1
301,563 - GFF3478 0.85 +0.6
301,563 - GFF3478 0.85 +1.6
301,601 + GFF3478 0.88 +1.6
301,601 + GFF3478 0.88 +0.1
301,602 - GFF3478 0.88 +0.7
301,602 - GFF3478 0.88 +0.5
301,602 - GFF3478 0.88 +1.1
301,602 - GFF3478 0.88 -0.4
301,724 + -0.2
301,724 + -1.4
301,724 + +0.2
301,724 + -0.5
301,725 - -0.0
301,725 - -0.7
301,725 - +1.1
301,725 - +0.1
301,725 - +0.5
301,725 - +0.3
301,757 + -0.5
301,758 - -0.0
301,758 - -0.5
301,803 + -0.7
301,803 + -0.3
301,803 + -0.2
302,049 + GFF3479 0.16 -0.3
302,049 + GFF3479 0.16 +0.5
302,050 - GFF3479 0.16 -1.0
302,050 - GFF3479 0.16 -0.4
302,340 + GFF3479 0.41 -0.2
302,341 - GFF3479 0.41 -0.1
302,341 - GFF3479 0.41 +0.1
302,341 - GFF3479 0.41 -0.7
302,643 + GFF3479 0.67 -0.7
302,644 - GFF3479 0.67 -0.7
302,742 + GFF3479 0.76 -0.4
302,742 + GFF3479 0.76 -0.3
302,743 - GFF3479 0.76 -1.6
302,743 - GFF3479 0.76 -1.0
302,743 - GFF3479 0.76 +1.6
302,743 - GFF3479 0.76 -2.0
302,743 - GFF3479 0.76 +0.7

Or see this region's nucleotide sequence