Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3428 and GFF3429 are separated by 16 nucleotides GFF3429 and GFF3430 are separated by 7 nucleotides GFF3430 and GFF3431 are separated by 186 nucleotides
GFF3428 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 251,484 to 252,296
GFF3428
GFF3429 - Transcriptional regulator, AraC family, at 252,313 to 253,233
GFF3429
GFF3430 - Transcriptional regulator, LysR family, at 253,241 to 254,143
GFF3430
GFF3431 - Glutaryl-CoA dehydrogenase (EC 1.3.8.6), at 254,330 to 255,529
GFF3431
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 252.294 kb on + strand at 252.305 kb on + strand at 252.305 kb on + strand at 252.731 kb on + strand, within GFF3429 at 252.731 kb on + strand, within GFF3429 at 252.732 kb on - strand, within GFF3429 at 252.740 kb on + strand, within GFF3429 at 252.741 kb on - strand, within GFF3429 at 253.058 kb on + strand, within GFF3429 at 253.058 kb on + strand, within GFF3429 at 253.109 kb on + strand, within GFF3429 at 253.109 kb on + strand, within GFF3429 at 253.110 kb on - strand, within GFF3429 at 253.110 kb on - strand, within GFF3429 at 253.326 kb on + strand at 253.326 kb on + strand at 253.326 kb on + strand at 253.326 kb on + strand at 253.327 kb on - strand at 253.327 kb on - strand at 253.327 kb on - strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.329 kb on + strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.470 kb on + strand, within GFF3430 at 253.470 kb on + strand, within GFF3430 at 253.471 kb on - strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.158 kb on + strand at 254.218 kb on + strand at 254.219 kb on - strand at 254.219 kb on - strand at 254.229 kb on - strand at 254.229 kb on - strand at 254.273 kb on + strand at 254.274 kb on - strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.427 kb on - strand at 254.546 kb on + strand, within GFF3431 at 254.546 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.576 kb on + strand, within GFF3431 at 254.577 kb on - strand, within GFF3431
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 252,294 + +0.5 252,305 + -0.6 252,305 + +0.5 252,731 + GFF3429 0.45 +1.3 252,731 + GFF3429 0.45 +0.1 252,732 - GFF3429 0.45 -0.5 252,740 + GFF3429 0.46 -0.5 252,741 - GFF3429 0.46 +0.2 253,058 + GFF3429 0.81 -0.4 253,058 + GFF3429 0.81 -0.1 253,109 + GFF3429 0.86 +0.9 253,109 + GFF3429 0.86 +0.3 253,110 - GFF3429 0.87 +1.2 253,110 - GFF3429 0.87 -2.6 253,326 + -0.6 253,326 + -0.1 253,326 + -1.2 253,326 + +1.7 253,327 - -0.3 253,327 - -1.1 253,327 - +0.8 253,329 + -0.6 253,329 + +0.0 253,329 + -1.1 253,329 + -0.2 253,329 + -1.1 253,329 + -0.7 253,329 + -0.5 253,329 + -0.1 253,330 - +0.5 253,330 - -0.7 253,330 - -1.2 253,330 - +0.2 253,330 - +0.1 253,470 + GFF3430 0.25 -0.7 253,470 + GFF3430 0.25 -0.2 253,471 - GFF3430 0.25 +0.5 253,704 + GFF3430 0.51 +0.1 253,704 + GFF3430 0.51 -0.5 253,705 - GFF3430 0.51 -0.6 253,705 - GFF3430 0.51 +0.5 253,938 + GFF3430 0.77 -0.3 253,938 + GFF3430 0.77 +0.5 253,938 + GFF3430 0.77 -0.8 253,938 + GFF3430 0.77 -0.1 253,938 + GFF3430 0.77 -0.7 254,076 + +0.8 254,076 + -0.4 254,076 + -0.2 254,076 + +0.7 254,076 + -0.1 254,077 - -0.5 254,077 - -0.2 254,077 - -0.4 254,077 - -0.2 254,077 - -0.4 254,145 + -0.2 254,145 + +1.3 254,145 + -0.6 254,145 + +1.1 254,145 + -0.7 254,145 + +0.1 254,145 + -0.7 254,145 + -0.1 254,145 + -0.2 254,145 + -0.3 254,145 + -1.5 254,146 - -0.5 254,146 - -0.3 254,146 - -1.6 254,146 - +0.2 254,146 - -0.6 254,158 + -0.4 254,218 + +0.2 254,219 - -0.4 254,219 - -0.7 254,229 - +3.1 254,229 - +0.2 254,273 + -1.6 254,274 - -0.3 254,426 + +1.0 254,426 + -0.8 254,426 + +0.8 254,427 - +0.4 254,546 + GFF3431 0.18 -1.1 254,546 + GFF3431 0.18 -0.2 254,567 + GFF3431 0.20 +0.0 254,567 + GFF3431 0.20 +0.2 254,567 + GFF3431 0.20 -0.2 254,568 - GFF3431 0.20 +0.5 254,568 - GFF3431 0.20 +0.1 254,568 - GFF3431 0.20 -0.2 254,568 - GFF3431 0.20 +1.1 254,576 + GFF3431 0.20 +1.8 254,577 - GFF3431 0.21 -0.1
Or see this region's nucleotide sequence