Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF287 and GFF288 are separated by 181 nucleotides GFF288 and GFF289 are separated by 387 nucleotides GFF289 and GFF290 are separated by 19 nucleotides GFF290 and GFF291 overlap by 1 nucleotides
GFF287 - hypothetical protein, at 322,045 to 323,301
GFF287
GFF288 - no description, at 323,483 to 323,812
GFF288
GFF289 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 324,200 to 325,045
GFF289
GFF290 - Cell division coordinator CpoB, at 325,065 to 325,814
GFF290
GFF291 - Tol-Pal system peptidoglycan-associated lipoprotein PAL, at 325,814 to 326,332
GFF291
Position (kb) 324
325
326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 323.296 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.360 kb on + strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.361 kb on - strand at 323.377 kb on - strand at 323.386 kb on - strand at 323.387 kb on - strand at 323.410 kb on + strand at 323.421 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.479 kb on + strand at 323.509 kb on + strand at 323.509 kb on + strand at 323.509 kb on + strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.510 kb on - strand at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.526 kb on + strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.527 kb on - strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.538 kb on + strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.539 kb on - strand, within GFF288 at 323.689 kb on + strand, within GFF288 at 323.689 kb on + strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.690 kb on - strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.691 kb on + strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.692 kb on - strand, within GFF288 at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.812 kb on + strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 323.813 kb on - strand at 324.017 kb on + strand at 324.017 kb on + strand at 324.017 kb on + strand at 324.018 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.079 kb on - strand at 324.196 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand at 324.204 kb on - strand at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.578 kb on + strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.579 kb on - strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.933 kb on + strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.934 kb on - strand, within GFF289 at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.966 kb on + strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 324.967 kb on - strand at 325.120 kb on + strand at 325.121 kb on - strand at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.273 kb on + strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.274 kb on - strand, within GFF290 at 325.288 kb on + strand, within GFF290 at 325.289 kb on - strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.327 kb on + strand, within GFF290 at 325.328 kb on - strand, within GFF290 at 325.328 kb on - strand, within GFF290 at 325.342 kb on + strand, within GFF290 at 325.824 kb on + strand at 325.824 kb on + strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.825 kb on - strand at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.992 kb on + strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291 at 325.993 kb on - strand, within GFF291
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 323,296 + +1.0 323,360 + -1.5 323,360 + -0.8 323,360 + -0.3 323,360 + +0.8 323,360 + +0.4 323,360 + +1.4 323,360 + -0.2 323,360 + -0.3 323,361 - +0.1 323,361 - -0.3 323,361 - +0.4 323,361 - -1.1 323,361 - -0.3 323,377 - -2.2 323,386 - +0.5 323,387 - -1.2 323,410 + -0.4 323,421 + -0.5 323,479 + -0.1 323,479 + -0.6 323,479 + -1.7 323,479 + -1.8 323,479 + -1.2 323,479 + -0.1 323,509 + +1.2 323,509 + +1.4 323,509 + -0.7 323,510 - -0.4 323,510 - +0.1 323,510 - -1.6 323,510 - -2.3 323,510 - +0.7 323,510 - -0.2 323,510 - -0.4 323,510 - -0.1 323,510 - +0.2 323,526 + GFF288 0.13 -0.2 323,526 + GFF288 0.13 +0.2 323,526 + GFF288 0.13 -0.9 323,526 + GFF288 0.13 +1.2 323,526 + GFF288 0.13 -0.2 323,526 + GFF288 0.13 -0.3 323,526 + GFF288 0.13 +0.2 323,526 + GFF288 0.13 -0.1 323,526 + GFF288 0.13 -0.4 323,526 + GFF288 0.13 +0.1 323,526 + GFF288 0.13 -1.7 323,526 + GFF288 0.13 +1.0 323,527 - GFF288 0.13 +0.3 323,527 - GFF288 0.13 -0.0 323,527 - GFF288 0.13 -0.5 323,527 - GFF288 0.13 -0.6 323,527 - GFF288 0.13 -0.6 323,527 - GFF288 0.13 -0.2 323,527 - GFF288 0.13 -0.3 323,527 - GFF288 0.13 -0.3 323,527 - GFF288 0.13 -1.6 323,527 - GFF288 0.13 -1.2 323,527 - GFF288 0.13 +0.3 323,538 + GFF288 0.17 +0.4 323,538 + GFF288 0.17 -0.5 323,538 + GFF288 0.17 +0.4 323,538 + GFF288 0.17 +1.1 323,538 + GFF288 0.17 -0.7 323,538 + GFF288 0.17 +1.3 323,538 + GFF288 0.17 +0.2 323,538 + GFF288 0.17 +0.5 323,538 + GFF288 0.17 +0.9 323,538 + GFF288 0.17 -1.5 323,538 + GFF288 0.17 -0.2 323,538 + GFF288 0.17 +0.4 323,539 - GFF288 0.17 +0.3 323,539 - GFF288 0.17 +0.5 323,539 - GFF288 0.17 +0.9 323,539 - GFF288 0.17 -0.6 323,539 - GFF288 0.17 +0.3 323,539 - GFF288 0.17 -0.0 323,539 - GFF288 0.17 -0.9 323,539 - GFF288 0.17 -1.3 323,539 - GFF288 0.17 -0.1 323,539 - GFF288 0.17 +0.7 323,689 + GFF288 0.62 +0.4 323,689 + GFF288 0.62 -0.3 323,690 - GFF288 0.63 -0.5 323,690 - GFF288 0.63 +0.2 323,690 - GFF288 0.63 -0.1 323,691 + GFF288 0.63 -0.5 323,691 + GFF288 0.63 -1.3 323,691 + GFF288 0.63 -0.9 323,691 + GFF288 0.63 -0.5 323,691 + GFF288 0.63 +1.4 323,691 + GFF288 0.63 +0.4 323,691 + GFF288 0.63 -0.5 323,691 + GFF288 0.63 +0.3 323,691 + GFF288 0.63 -1.7 323,691 + GFF288 0.63 -0.1 323,692 - GFF288 0.63 +1.1 323,692 - GFF288 0.63 -0.3 323,692 - GFF288 0.63 -0.5 323,692 - GFF288 0.63 -0.4 323,692 - GFF288 0.63 -0.1 323,692 - GFF288 0.63 -1.4 323,812 + +2.0 323,812 + -0.2 323,812 + +1.1 323,812 + +0.3 323,812 + +0.2 323,812 + -0.2 323,812 + -0.0 323,812 + -0.5 323,812 + -0.5 323,813 - -1.6 323,813 - -0.3 323,813 - -0.9 323,813 - +0.6 323,813 - -0.9 323,813 - +0.3 323,813 - -1.1 323,813 - +0.2 323,813 - -0.8 324,017 + -0.5 324,017 + +0.4 324,017 + +0.4 324,018 - -1.2 324,079 - +0.4 324,079 - +0.1 324,079 - +0.3 324,079 - -0.7 324,196 - -0.2 324,204 - +0.7 324,204 - +1.0 324,204 - +0.7 324,578 + GFF289 0.45 -0.4 324,578 + GFF289 0.45 +0.6 324,578 + GFF289 0.45 -0.8 324,578 + GFF289 0.45 -0.9 324,579 - GFF289 0.45 -1.6 324,579 - GFF289 0.45 +1.0 324,579 - GFF289 0.45 -1.2 324,579 - GFF289 0.45 -0.1 324,579 - GFF289 0.45 -2.3 324,933 + GFF289 0.87 -0.2 324,933 + GFF289 0.87 +0.5 324,933 + GFF289 0.87 -0.2 324,933 + GFF289 0.87 -0.9 324,933 + GFF289 0.87 -0.7 324,933 + GFF289 0.87 -1.1 324,934 - GFF289 0.87 -0.4 324,934 - GFF289 0.87 -0.9 324,934 - GFF289 0.87 -0.6 324,966 + -0.9 324,966 + -0.2 324,966 + -0.7 324,966 + +0.4 324,966 + -1.2 324,966 + -1.2 324,967 - -0.8 324,967 - -0.6 324,967 - +0.1 324,967 - +0.1 324,967 - +0.2 325,120 + +0.4 325,121 - -1.1 325,273 + GFF290 0.28 -0.9 325,273 + GFF290 0.28 -1.1 325,273 + GFF290 0.28 -0.4 325,273 + GFF290 0.28 +2.4 325,273 + GFF290 0.28 -2.1 325,273 + GFF290 0.28 -0.6 325,273 + GFF290 0.28 +2.4 325,273 + GFF290 0.28 -0.8 325,273 + GFF290 0.28 -1.7 325,274 - GFF290 0.28 -0.7 325,274 - GFF290 0.28 -0.2 325,274 - GFF290 0.28 +1.4 325,274 - GFF290 0.28 -2.0 325,274 - GFF290 0.28 +1.2 325,274 - GFF290 0.28 -1.2 325,274 - GFF290 0.28 -0.8 325,274 - GFF290 0.28 -1.3 325,274 - GFF290 0.28 -0.7 325,274 - GFF290 0.28 +0.1 325,274 - GFF290 0.28 -0.2 325,288 + GFF290 0.30 -0.9 325,289 - GFF290 0.30 -0.4 325,327 + GFF290 0.35 -1.6 325,327 + GFF290 0.35 -0.3 325,327 + GFF290 0.35 -1.1 325,327 + GFF290 0.35 +0.0 325,328 - GFF290 0.35 -0.6 325,328 - GFF290 0.35 -1.8 325,342 + GFF290 0.37 -1.2 325,824 + -0.4 325,824 + -0.6 325,825 - -0.3 325,825 - -0.1 325,825 - +0.2 325,825 - +0.8 325,992 + GFF291 0.34 -0.7 325,992 + GFF291 0.34 -0.8 325,992 + GFF291 0.34 -0.1 325,992 + GFF291 0.34 -1.9 325,993 - GFF291 0.34 -1.4 325,993 - GFF291 0.34 -0.0 325,993 - GFF291 0.34 -2.2 325,993 - GFF291 0.34 -2.2 325,993 - GFF291 0.34 -2.9 325,993 - GFF291 0.34 +1.7 325,993 - GFF291 0.34 -1.4 325,993 - GFF291 0.34 -1.1 325,993 - GFF291 0.34 +2.4 325,993 - GFF291 0.34 -0.2 325,993 - GFF291 0.34 -1.8 325,993 - GFF291 0.34 -0.8 325,993 - GFF291 0.34 -0.6 325,993 - GFF291 0.34 -0.4
Or see this region's nucleotide sequence