Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2719 and GFF2720 are separated by 59 nucleotides GFF2720 and GFF2721 are separated by 28 nucleotides GFF2721 and GFF2722 are separated by 125 nucleotides
GFF2719 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 152,302 to 153,099
GFF2719
GFF2720 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 153,159 to 153,812
GFF2720
GFF2721 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 153,841 to 154,512
GFF2721
GFF2722 - Transcriptional regulator, LysR family, at 154,638 to 155,594
GFF2722
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 152.910 kb on - strand, within GFF2719 at 152.945 kb on + strand, within GFF2719 at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.236 kb on - strand, within GFF2720 at 153.367 kb on + strand, within GFF2720 at 153.367 kb on + strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.368 kb on - strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.436 kb on + strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.437 kb on - strand, within GFF2720 at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.806 kb on + strand at 153.807 kb on - strand at 153.807 kb on - strand at 153.807 kb on - strand at 153.857 kb on + strand at 153.876 kb on - strand at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.920 kb on + strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.921 kb on - strand, within GFF2721 at 153.941 kb on + strand, within GFF2721 at 153.942 kb on - strand, within GFF2721 at 153.942 kb on - strand, within GFF2721 at 154.070 kb on + strand, within GFF2721 at 154.202 kb on + strand, within GFF2721 at 154.203 kb on - strand, within GFF2721 at 154.203 kb on - strand, within GFF2721 at 154.203 kb on - strand, within GFF2721 at 154.203 kb on - strand, within GFF2721 at 154.334 kb on + strand, within GFF2721 at 154.334 kb on + strand, within GFF2721 at 154.334 kb on + strand, within GFF2721 at 154.334 kb on + strand, within GFF2721 at 154.335 kb on - strand, within GFF2721 at 154.335 kb on - strand, within GFF2721 at 154.335 kb on - strand, within GFF2721 at 154.335 kb on - strand, within GFF2721 at 154.505 kb on + strand at 154.505 kb on + strand at 154.505 kb on + strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.506 kb on - strand at 154.549 kb on + strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.550 kb on - strand at 154.580 kb on + strand at 154.605 kb on - strand at 154.605 kb on - strand at 154.605 kb on - strand at 154.617 kb on - strand at 154.617 kb on - strand at 154.617 kb on - strand at 154.621 kb on - strand at 154.621 kb on - strand at 154.762 kb on + strand, within GFF2722 at 154.762 kb on + strand, within GFF2722 at 154.762 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.765 kb on + strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.766 kb on - strand, within GFF2722 at 154.969 kb on + strand, within GFF2722 at 154.969 kb on + strand, within GFF2722 at 154.969 kb on + strand, within GFF2722 at 154.969 kb on + strand, within GFF2722 at 154.969 kb on + strand, within GFF2722 at 154.970 kb on - strand, within GFF2722 at 154.970 kb on - strand, within GFF2722 at 154.970 kb on - strand, within GFF2722 at 155.005 kb on + strand, within GFF2722 at 155.068 kb on + strand, within GFF2722 at 155.068 kb on + strand, within GFF2722 at 155.068 kb on + strand, within GFF2722 at 155.069 kb on - strand, within GFF2722 at 155.227 kb on + strand, within GFF2722
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 152,910 - GFF2719 0.76 +0.6 152,945 + GFF2719 0.81 -1.5 153,104 + +0.7 153,104 + -0.1 153,104 + -0.2 153,104 + -0.4 153,105 - -0.0 153,105 - -0.1 153,105 - -0.4 153,105 - -0.8 153,105 - +0.8 153,105 - -0.4 153,235 + GFF2720 0.12 -0.0 153,235 + GFF2720 0.12 -0.4 153,235 + GFF2720 0.12 -0.9 153,236 - GFF2720 0.12 -1.1 153,367 + GFF2720 0.32 +0.5 153,367 + GFF2720 0.32 +0.0 153,368 - GFF2720 0.32 +0.7 153,368 - GFF2720 0.32 -1.3 153,368 - GFF2720 0.32 -0.8 153,368 - GFF2720 0.32 -0.3 153,436 + GFF2720 0.42 -1.1 153,436 + GFF2720 0.42 -0.6 153,436 + GFF2720 0.42 -0.3 153,436 + GFF2720 0.42 -1.3 153,436 + GFF2720 0.42 -0.0 153,437 - GFF2720 0.43 -0.6 153,437 - GFF2720 0.43 -0.1 153,437 - GFF2720 0.43 -0.0 153,437 - GFF2720 0.43 +1.1 153,437 - GFF2720 0.43 -0.8 153,437 - GFF2720 0.43 +0.2 153,437 - GFF2720 0.43 -1.0 153,437 - GFF2720 0.43 -0.2 153,806 + -0.0 153,806 + -1.6 153,806 + -0.2 153,806 + -0.4 153,806 + +0.3 153,807 - +1.4 153,807 - +0.5 153,807 - -0.4 153,857 + -1.8 153,876 - -0.9 153,920 + GFF2721 0.12 -0.7 153,920 + GFF2721 0.12 -1.1 153,920 + GFF2721 0.12 -0.3 153,920 + GFF2721 0.12 -1.1 153,920 + GFF2721 0.12 -0.5 153,920 + GFF2721 0.12 -0.2 153,920 + GFF2721 0.12 -1.4 153,920 + GFF2721 0.12 -0.0 153,921 - GFF2721 0.12 +0.6 153,921 - GFF2721 0.12 -0.0 153,921 - GFF2721 0.12 +0.2 153,921 - GFF2721 0.12 -0.7 153,941 + GFF2721 0.15 -2.0 153,942 - GFF2721 0.15 -0.1 153,942 - GFF2721 0.15 -0.0 154,070 + GFF2721 0.34 -0.9 154,202 + GFF2721 0.54 -0.7 154,203 - GFF2721 0.54 -2.2 154,203 - GFF2721 0.54 +0.5 154,203 - GFF2721 0.54 -0.1 154,203 - GFF2721 0.54 +0.0 154,334 + GFF2721 0.73 +0.6 154,334 + GFF2721 0.73 -0.3 154,334 + GFF2721 0.73 -0.9 154,334 + GFF2721 0.73 -0.8 154,335 - GFF2721 0.74 +1.0 154,335 - GFF2721 0.74 -0.8 154,335 - GFF2721 0.74 -0.7 154,335 - GFF2721 0.74 -2.5 154,505 + -0.8 154,505 + +0.7 154,505 + +0.5 154,506 - +1.9 154,506 - +1.3 154,506 - +0.5 154,506 - -0.5 154,506 - +3.3 154,506 - +1.7 154,506 - -2.2 154,506 - -0.2 154,549 + -1.2 154,550 - -0.2 154,550 - -0.4 154,550 - +0.2 154,550 - -0.0 154,550 - -0.8 154,550 - -0.2 154,550 - +1.2 154,580 + -0.2 154,605 - -0.3 154,605 - -0.3 154,605 - +0.7 154,617 - -0.7 154,617 - +0.2 154,617 - +1.1 154,621 - +0.1 154,621 - -0.4 154,762 + GFF2722 0.13 -0.3 154,762 + GFF2722 0.13 -0.6 154,762 + GFF2722 0.13 -0.2 154,765 + GFF2722 0.13 -0.3 154,765 + GFF2722 0.13 -0.0 154,765 + GFF2722 0.13 -0.6 154,765 + GFF2722 0.13 -0.2 154,765 + GFF2722 0.13 +0.6 154,765 + GFF2722 0.13 -0.6 154,765 + GFF2722 0.13 -0.6 154,765 + GFF2722 0.13 -0.3 154,765 + GFF2722 0.13 +2.4 154,765 + GFF2722 0.13 -0.2 154,765 + GFF2722 0.13 -1.4 154,766 - GFF2722 0.13 -1.7 154,766 - GFF2722 0.13 -2.8 154,766 - GFF2722 0.13 +0.3 154,766 - GFF2722 0.13 +0.7 154,766 - GFF2722 0.13 +0.2 154,766 - GFF2722 0.13 +0.6 154,766 - GFF2722 0.13 -0.9 154,766 - GFF2722 0.13 +0.1 154,766 - GFF2722 0.13 -0.0 154,766 - GFF2722 0.13 -0.6 154,969 + GFF2722 0.35 -0.9 154,969 + GFF2722 0.35 -0.2 154,969 + GFF2722 0.35 +0.2 154,969 + GFF2722 0.35 -0.4 154,969 + GFF2722 0.35 -0.4 154,970 - GFF2722 0.35 +0.1 154,970 - GFF2722 0.35 -0.1 154,970 - GFF2722 0.35 -0.1 155,005 + GFF2722 0.38 -0.1 155,068 + GFF2722 0.45 -0.5 155,068 + GFF2722 0.45 -0.9 155,068 + GFF2722 0.45 +0.2 155,069 - GFF2722 0.45 +0.8 155,227 + GFF2722 0.62 -0.2
Or see this region's nucleotide sequence