Strain Fitness in Variovorax sp. SCN45 around GFF254

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF253 and GFF254 are separated by 31 nucleotidesGFF254 and GFF255 are separated by 40 nucleotidesGFF255 and GFF256 are separated by 65 nucleotides GFF253 - Transcriptional regulator, LysR family, at 284,828 to 285,727 GFF253 GFF254 - N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17), type 2, at 285,759 to 286,979 GFF254 GFF255 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 287,020 to 287,853 GFF255 GFF256 - Twitching motility protein PilT, at 287,919 to 289,067 GFF256 Position (kb) 285 286 287Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 284.816 kb on - strandat 284.837 kb on + strandat 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.326 kb on + strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.327 kb on - strand, within GFF253at 285.359 kb on + strand, within GFF253at 285.359 kb on + strand, within GFF253at 285.359 kb on + strand, within GFF253at 285.359 kb on + strand, within GFF253at 285.360 kb on - strand, within GFF253at 285.360 kb on - strand, within GFF253at 285.360 kb on - strand, within GFF253at 285.360 kb on - strand, within GFF253at 285.360 kb on - strand, within GFF253at 285.369 kb on - strand, within GFF253at 285.369 kb on - strand, within GFF253at 285.369 kb on - strand, within GFF253at 285.369 kb on - strand, within GFF253at 285.369 kb on - strand, within GFF253at 285.653 kb on + strandat 285.654 kb on - strandat 285.708 kb on + strandat 285.708 kb on + strandat 285.708 kb on + strandat 285.708 kb on + strandat 285.708 kb on + strandat 285.708 kb on + strandat 285.709 kb on - strandat 285.709 kb on - strandat 286.002 kb on + strand, within GFF254at 286.002 kb on + strand, within GFF254at 286.137 kb on + strand, within GFF254at 286.137 kb on + strand, within GFF254at 286.138 kb on - strand, within GFF254at 286.138 kb on - strand, within GFF254at 286.138 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.147 kb on - strand, within GFF254at 286.182 kb on + strand, within GFF254at 286.182 kb on + strand, within GFF254at 286.182 kb on + strand, within GFF254at 286.183 kb on - strand, within GFF254at 286.185 kb on + strand, within GFF254at 286.185 kb on + strand, within GFF254at 286.186 kb on - strand, within GFF254at 286.186 kb on - strand, within GFF254at 286.186 kb on - strand, within GFF254at 286.186 kb on - strand, within GFF254at 286.380 kb on + strand, within GFF254at 286.380 kb on + strand, within GFF254at 286.380 kb on + strand, within GFF254at 286.381 kb on - strand, within GFF254at 286.381 kb on - strand, within GFF254at 286.381 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.393 kb on - strand, within GFF254at 286.525 kb on + strand, within GFF254at 286.526 kb on - strand, within GFF254at 286.564 kb on - strand, within GFF254at 286.572 kb on + strand, within GFF254at 286.572 kb on + strand, within GFF254at 286.572 kb on + strand, within GFF254at 286.572 kb on + strand, within GFF254at 286.573 kb on - strand, within GFF254at 286.573 kb on - strand, within GFF254at 286.573 kb on - strand, within GFF254at 286.573 kb on - strand, within GFF254at 286.662 kb on + strand, within GFF254at 286.663 kb on - strand, within GFF254at 286.663 kb on - strand, within GFF254at 286.663 kb on - strand, within GFF254at 286.737 kb on + strand, within GFF254at 286.737 kb on + strand, within GFF254at 286.737 kb on + strand, within GFF254at 286.737 kb on + strand, within GFF254at 286.738 kb on - strand, within GFF254at 286.753 kb on + strand, within GFF254at 286.800 kb on + strand, within GFF254at 286.800 kb on + strand, within GFF254at 286.800 kb on + strand, within GFF254at 286.801 kb on - strand, within GFF254at 286.801 kb on - strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.809 kb on + strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.810 kb on - strand, within GFF254at 286.836 kb on + strand, within GFF254at 286.836 kb on + strand, within GFF254at 286.836 kb on + strand, within GFF254at 286.836 kb on + strand, within GFF254at 286.837 kb on - strand, within GFF254at 286.837 kb on - strand, within GFF254at 286.837 kb on - strand, within GFF254at 286.837 kb on - strand, within GFF254at 286.837 kb on - strand, within GFF254at 287.423 kb on - strand, within GFF255at 287.644 kb on + strand, within GFF255at 287.644 kb on + strand, within GFF255at 287.644 kb on + strand, within GFF255at 287.644 kb on + strand, within GFF255at 287.644 kb on + strand, within GFF255at 287.693 kb on - strand, within GFF255at 287.767 kb on + strand, within GFF255at 287.776 kb on + strandat 287.776 kb on + strandat 287.777 kb on - strandat 287.972 kb on + strandat 287.972 kb on + strandat 287.972 kb on + strandat 287.973 kb on - strandat 287.973 kb on - strandat 287.973 kb on - strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.976 kb on + strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strandat 287.977 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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284,816 - +0.4
284,837 + -0.2
285,326 + GFF253 0.55 -0.8
285,326 + GFF253 0.55 -0.0
285,326 + GFF253 0.55 -0.3
285,326 + GFF253 0.55 -0.5
285,326 + GFF253 0.55 -0.5
285,326 + GFF253 0.55 -0.9
285,326 + GFF253 0.55 -0.2
285,326 + GFF253 0.55 +0.7
285,326 + GFF253 0.55 -0.0
285,326 + GFF253 0.55 +0.4
285,326 + GFF253 0.55 -0.4
285,326 + GFF253 0.55 +0.4
285,327 - GFF253 0.55 -1.1
285,327 - GFF253 0.55 +1.9
285,327 - GFF253 0.55 +0.7
285,327 - GFF253 0.55 -0.3
285,327 - GFF253 0.55 +0.2
285,327 - GFF253 0.55 -0.2
285,327 - GFF253 0.55 -0.4
285,327 - GFF253 0.55 -0.7
285,327 - GFF253 0.55 -0.4
285,327 - GFF253 0.55 -0.5
285,359 + GFF253 0.59 -2.4
285,359 + GFF253 0.59 -0.3
285,359 + GFF253 0.59 +0.3
285,359 + GFF253 0.59 -0.5
285,360 - GFF253 0.59 +0.0
285,360 - GFF253 0.59 +0.3
285,360 - GFF253 0.59 +0.9
285,360 - GFF253 0.59 +0.1
285,360 - GFF253 0.59 -1.6
285,369 - GFF253 0.60 -0.3
285,369 - GFF253 0.60 +0.4
285,369 - GFF253 0.60 -0.7
285,369 - GFF253 0.60 +0.0
285,369 - GFF253 0.60 +0.1
285,653 + +0.1
285,654 - +0.7
285,708 + +0.3
285,708 + +0.5
285,708 + -0.5
285,708 + -0.2
285,708 + +0.1
285,708 + +0.2
285,709 - -0.2
285,709 - -1.2
286,002 + GFF254 0.20 -1.3
286,002 + GFF254 0.20 -0.9
286,137 + GFF254 0.31 -1.2
286,137 + GFF254 0.31 +0.7
286,138 - GFF254 0.31 -0.7
286,138 - GFF254 0.31 -1.0
286,138 - GFF254 0.31 -0.2
286,147 - GFF254 0.32 -1.2
286,147 - GFF254 0.32 -0.6
286,147 - GFF254 0.32 -0.8
286,147 - GFF254 0.32 +0.7
286,147 - GFF254 0.32 -2.4
286,147 - GFF254 0.32 -1.3
286,182 + GFF254 0.35 -1.1
286,182 + GFF254 0.35 -0.4
286,182 + GFF254 0.35 -1.1
286,183 - GFF254 0.35 -1.0
286,185 + GFF254 0.35 -1.7
286,185 + GFF254 0.35 -0.6
286,186 - GFF254 0.35 +0.2
286,186 - GFF254 0.35 -0.6
286,186 - GFF254 0.35 -0.0
286,186 - GFF254 0.35 -0.4
286,380 + GFF254 0.51 -0.9
286,380 + GFF254 0.51 +0.2
286,380 + GFF254 0.51 -1.8
286,381 - GFF254 0.51 -1.1
286,381 - GFF254 0.51 -1.2
286,381 - GFF254 0.51 +0.1
286,393 - GFF254 0.52 -1.0
286,393 - GFF254 0.52 -0.3
286,393 - GFF254 0.52 -2.2
286,393 - GFF254 0.52 -0.5
286,393 - GFF254 0.52 -1.3
286,393 - GFF254 0.52 -0.6
286,525 + GFF254 0.63 +0.8
286,526 - GFF254 0.63 -0.9
286,564 - GFF254 0.66 -1.0
286,572 + GFF254 0.67 -1.4
286,572 + GFF254 0.67 -1.4
286,572 + GFF254 0.67 -0.3
286,572 + GFF254 0.67 +0.1
286,573 - GFF254 0.67 -0.7
286,573 - GFF254 0.67 -2.1
286,573 - GFF254 0.67 -1.0
286,573 - GFF254 0.67 -0.2
286,662 + GFF254 0.74 -1.7
286,663 - GFF254 0.74 +0.6
286,663 - GFF254 0.74 -0.4
286,663 - GFF254 0.74 -1.2
286,737 + GFF254 0.80 -0.9
286,737 + GFF254 0.80 -0.2
286,737 + GFF254 0.80 -0.7
286,737 + GFF254 0.80 -1.3
286,738 - GFF254 0.80 -0.5
286,753 + GFF254 0.81 -0.4
286,800 + GFF254 0.85 -1.1
286,800 + GFF254 0.85 -1.3
286,800 + GFF254 0.85 -1.7
286,801 - GFF254 0.85 -1.0
286,801 - GFF254 0.85 -0.2
286,809 + GFF254 0.86 -2.0
286,809 + GFF254 0.86 -0.7
286,809 + GFF254 0.86 -1.2
286,809 + GFF254 0.86 +0.4
286,809 + GFF254 0.86 -0.6
286,809 + GFF254 0.86 -0.8
286,809 + GFF254 0.86 -0.8
286,809 + GFF254 0.86 -0.9
286,810 - GFF254 0.86 -1.3
286,810 - GFF254 0.86 -0.6
286,810 - GFF254 0.86 -1.1
286,810 - GFF254 0.86 -2.8
286,810 - GFF254 0.86 -1.8
286,810 - GFF254 0.86 -0.7
286,810 - GFF254 0.86 +0.8
286,810 - GFF254 0.86 -0.2
286,810 - GFF254 0.86 -1.3
286,836 + GFF254 0.88 -0.8
286,836 + GFF254 0.88 -2.5
286,836 + GFF254 0.88 -1.7
286,836 + GFF254 0.88 -1.8
286,837 - GFF254 0.88 -1.5
286,837 - GFF254 0.88 -1.2
286,837 - GFF254 0.88 -0.6
286,837 - GFF254 0.88 -0.6
286,837 - GFF254 0.88 -1.2
287,423 - GFF255 0.48 -1.8
287,644 + GFF255 0.75 -0.2
287,644 + GFF255 0.75 -1.4
287,644 + GFF255 0.75 -1.5
287,644 + GFF255 0.75 -1.2
287,644 + GFF255 0.75 -0.6
287,693 - GFF255 0.81 -1.9
287,767 + GFF255 0.90 -1.8
287,776 + -0.9
287,776 + -0.6
287,777 - +0.1
287,972 + -1.0
287,972 + -0.4
287,972 + -0.3
287,973 - -0.4
287,973 - +0.1
287,973 - -1.9
287,976 + +0.6
287,976 + +0.4
287,976 + -0.6
287,976 + +0.1
287,976 + -2.5
287,976 + +0.8
287,976 + +0.4
287,976 + +0.0
287,976 + +0.1
287,976 + -0.9
287,976 + +1.0
287,977 - -0.4
287,977 - -0.2
287,977 - +0.6
287,977 - -1.3
287,977 - +0.8
287,977 - +0.3
287,977 - -0.3
287,977 - -1.5
287,977 - +0.8
287,977 - -0.2
287,977 - -0.6
287,977 - -0.2
287,977 - +0.8
287,977 - -0.5
287,977 - -0.6
287,977 - -0.5
287,977 - -1.0
287,977 - -0.0
287,977 - +0.0
287,977 - -0.3
287,977 - -0.0

Or see this region's nucleotide sequence