Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2471 and GFF2472 are separated by 34 nucleotides GFF2472 and GFF2473 overlap by 4 nucleotides GFF2473 and GFF2474 are separated by 214 nucleotides GFF2474 and GFF2475 overlap by 4 nucleotides
GFF2471 - Outer membrane porin protein 32 precursor, at 530,824 to 531,981
GFF2471
GFF2472 - no description, at 532,016 to 532,285
GFF2472
GFF2473 - hypothetical protein, at 532,282 to 532,893
GFF2473
GFF2474 - Two-component transcriptional response regulator SMc02366, LuxR family, at 533,108 to 533,791
GFF2474
GFF2475 - no description, at 533,788 to 535,158
GFF2475
Position (kb) 532
533 Strain fitness (log2 ratio) -2
-1
0
1 at 531.295 kb on + strand, within GFF2471 at 531.352 kb on + strand, within GFF2471 at 531.353 kb on - strand, within GFF2471 at 531.353 kb on - strand, within GFF2471 at 531.922 kb on + strand at 531.922 kb on + strand at 531.922 kb on + strand at 531.952 kb on + strand at 532.615 kb on + strand, within GFF2473 at 532.615 kb on + strand, within GFF2473 at 532.822 kb on + strand, within GFF2473 at 532.822 kb on + strand, within GFF2473 at 532.823 kb on - strand, within GFF2473 at 532.823 kb on - strand, within GFF2473 at 532.891 kb on + strand at 532.891 kb on + strand at 532.892 kb on - strand at 532.892 kb on - strand at 532.916 kb on - strand at 532.953 kb on + strand at 532.954 kb on - strand at 533.055 kb on + strand at 533.095 kb on - strand at 533.095 kb on - strand at 533.095 kb on - strand at 533.153 kb on + strand at 533.405 kb on + strand, within GFF2474 at 533.405 kb on + strand, within GFF2474 at 533.406 kb on - strand, within GFF2474 at 533.658 kb on - strand, within GFF2474 at 533.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 531,295 + GFF2471 0.41 +0.2 531,352 + GFF2471 0.46 -0.7 531,353 - GFF2471 0.46 +0.6 531,353 - GFF2471 0.46 -0.6 531,922 + -1.5 531,922 + +1.6 531,922 + -0.5 531,952 + +0.1 532,615 + GFF2473 0.54 -0.3 532,615 + GFF2473 0.54 +0.3 532,822 + GFF2473 0.88 -1.2 532,822 + GFF2473 0.88 -1.8 532,823 - GFF2473 0.88 -0.0 532,823 - GFF2473 0.88 -0.8 532,891 + -0.5 532,891 + -0.4 532,892 - -0.9 532,892 - -0.3 532,916 - -0.0 532,953 + -0.7 532,954 - -0.7 533,055 + -0.4 533,095 - -0.7 533,095 - -0.9 533,095 - +0.9 533,153 + +0.5 533,405 + GFF2474 0.43 -0.8 533,405 + GFF2474 0.43 +0.4 533,406 - GFF2474 0.44 +0.1 533,658 - GFF2474 0.80 +1.3 533,843 - +0.6
Or see this region's nucleotide sequence