Strain Fitness in Variovorax sp. SCN45 around GFF24

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF22 and GFF23 are separated by 18 nucleotidesGFF23 and GFF24 are separated by 8 nucleotidesGFF24 and GFF25 are separated by 140 nucleotides GFF22 - hypothetical protein, at 23,628 to 26,267 GFF22 GFF23 - no description, at 26,286 to 26,720 GFF23 GFF24 - VgrG protein, at 26,729 to 29,071 GFF24 GFF25 - Alcohol dehydrogenase (EC 1.1.1.1), at 29,212 to 30,348 GFF25 Position (kb) 26 27 28 29 30Strain fitness (log2 ratio) -2 -1 0 1at 26.359 kb on + strand, within GFF23at 26.638 kb on + strand, within GFF23at 26.638 kb on + strand, within GFF23at 26.639 kb on - strand, within GFF23at 26.757 kb on + strandat 26.757 kb on + strandat 26.758 kb on - strandat 26.778 kb on + strandat 26.850 kb on + strandat 26.850 kb on + strandat 26.851 kb on - strandat 26.899 kb on - strandat 26.899 kb on - strandat 27.003 kb on + strand, within GFF24at 27.015 kb on + strand, within GFF24at 27.015 kb on + strand, within GFF24at 27.036 kb on + strand, within GFF24at 27.036 kb on + strand, within GFF24at 27.036 kb on + strand, within GFF24at 27.057 kb on + strand, within GFF24at 27.057 kb on + strand, within GFF24at 27.057 kb on + strand, within GFF24at 27.058 kb on - strand, within GFF24at 27.058 kb on - strand, within GFF24at 27.058 kb on - strand, within GFF24at 27.058 kb on - strand, within GFF24at 27.078 kb on + strand, within GFF24at 27.078 kb on + strand, within GFF24at 27.283 kb on - strand, within GFF24at 27.486 kb on + strand, within GFF24at 27.486 kb on + strand, within GFF24at 27.486 kb on + strand, within GFF24at 27.486 kb on + strand, within GFF24at 28.000 kb on + strand, within GFF24at 28.087 kb on - strand, within GFF24at 28.087 kb on - strand, within GFF24at 28.087 kb on - strand, within GFF24at 28.177 kb on - strand, within GFF24at 28.177 kb on - strand, within GFF24at 28.305 kb on + strand, within GFF24at 28.305 kb on + strand, within GFF24at 28.306 kb on - strand, within GFF24at 28.314 kb on + strand, within GFF24at 28.314 kb on + strand, within GFF24at 28.314 kb on + strand, within GFF24at 28.518 kb on + strand, within GFF24at 28.518 kb on + strand, within GFF24at 28.578 kb on + strand, within GFF24at 28.635 kb on + strand, within GFF24at 28.662 kb on + strand, within GFF24at 28.663 kb on - strand, within GFF24at 28.663 kb on - strand, within GFF24at 28.663 kb on - strand, within GFF24at 28.698 kb on + strand, within GFF24at 28.698 kb on + strand, within GFF24at 28.699 kb on - strand, within GFF24at 28.776 kb on + strand, within GFF24at 28.777 kb on - strand, within GFF24at 28.777 kb on - strand, within GFF24at 28.806 kb on + strand, within GFF24at 28.807 kb on - strand, within GFF24at 28.807 kb on - strand, within GFF24at 28.807 kb on - strand, within GFF24at 28.812 kb on + strand, within GFF24at 28.812 kb on + strand, within GFF24at 28.812 kb on + strand, within GFF24at 28.812 kb on + strand, within GFF24at 28.813 kb on - strand, within GFF24at 28.813 kb on - strand, within GFF24at 29.213 kb on + strandat 29.213 kb on + strandat 29.214 kb on - strandat 29.241 kb on - strandat 29.241 kb on - strandat 29.241 kb on - strandat 29.335 kb on + strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25at 30.003 kb on - strand, within GFF25

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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26,359 + GFF23 0.17 -0.9
26,638 + GFF23 0.81 -0.6
26,638 + GFF23 0.81 +0.2
26,639 - GFF23 0.81 +0.3
26,757 + -0.2
26,757 + -0.1
26,758 - +0.4
26,778 + +0.1
26,850 + -0.4
26,850 + -0.3
26,851 - -0.7
26,899 - +1.7
26,899 - +0.1
27,003 + GFF24 0.12 -1.1
27,015 + GFF24 0.12 -0.8
27,015 + GFF24 0.12 -0.3
27,036 + GFF24 0.13 -0.2
27,036 + GFF24 0.13 -0.8
27,036 + GFF24 0.13 -0.1
27,057 + GFF24 0.14 -0.4
27,057 + GFF24 0.14 +0.3
27,057 + GFF24 0.14 -0.3
27,058 - GFF24 0.14 +0.3
27,058 - GFF24 0.14 -1.2
27,058 - GFF24 0.14 +0.2
27,058 - GFF24 0.14 -0.3
27,078 + GFF24 0.15 -0.8
27,078 + GFF24 0.15 +0.1
27,283 - GFF24 0.24 -0.4
27,486 + GFF24 0.32 -0.8
27,486 + GFF24 0.32 +1.4
27,486 + GFF24 0.32 -1.2
27,486 + GFF24 0.32 +0.3
28,000 + GFF24 0.54 -0.8
28,087 - GFF24 0.58 +0.4
28,087 - GFF24 0.58 -0.4
28,087 - GFF24 0.58 -0.5
28,177 - GFF24 0.62 +0.1
28,177 - GFF24 0.62 -0.7
28,305 + GFF24 0.67 -0.4
28,305 + GFF24 0.67 +0.2
28,306 - GFF24 0.67 -1.6
28,314 + GFF24 0.68 -0.3
28,314 + GFF24 0.68 +0.2
28,314 + GFF24 0.68 +0.3
28,518 + GFF24 0.76 -1.2
28,518 + GFF24 0.76 -0.3
28,578 + GFF24 0.79 +0.8
28,635 + GFF24 0.81 -0.4
28,662 + GFF24 0.83 -0.4
28,663 - GFF24 0.83 -0.4
28,663 - GFF24 0.83 -1.6
28,663 - GFF24 0.83 +0.4
28,698 + GFF24 0.84 -0.4
28,698 + GFF24 0.84 -0.6
28,699 - GFF24 0.84 -0.3
28,776 + GFF24 0.87 -1.8
28,777 - GFF24 0.87 -0.7
28,777 - GFF24 0.87 +0.5
28,806 + GFF24 0.89 +0.4
28,807 - GFF24 0.89 -0.3
28,807 - GFF24 0.89 -0.6
28,807 - GFF24 0.89 -0.4
28,812 + GFF24 0.89 +0.2
28,812 + GFF24 0.89 -0.2
28,812 + GFF24 0.89 -0.3
28,812 + GFF24 0.89 +0.0
28,813 - GFF24 0.89 +0.2
28,813 - GFF24 0.89 -1.2
29,213 + +0.1
29,213 + -0.2
29,214 - -1.4
29,241 - -1.1
29,241 - +0.1
29,241 - +0.7
29,335 + GFF25 0.11 -0.4
30,003 - GFF25 0.70 +0.2
30,003 - GFF25 0.70 -0.4
30,003 - GFF25 0.70 -1.1
30,003 - GFF25 0.70 +0.5
30,003 - GFF25 0.70 +0.0

Or see this region's nucleotide sequence