Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2307 and GFF2308 are separated by 13 nucleotides GFF2308 and GFF2309 are separated by 13 nucleotides GFF2309 and GFF2310 are separated by 54 nucleotides
GFF2307 - Heme O synthase, protoheme IX farnesyltransferase, COX10-CtaB, at 358,465 to 359,370
GFF2307
GFF2308 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, thiol-disulfide reductase involved in Cu(I) insertion into CoxII Cu(A) center, at 359,384 to 360,001
GFF2308
GFF2309 - DNA alkylation repair enzyme, at 360,015 to 360,842
GFF2309
GFF2310 - RNA polymerase sigma factor RpoH, at 360,897 to 361,838
GFF2310
Position (kb) 360
361 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 359.060 kb on - strand, within GFF2307 at 359.060 kb on - strand, within GFF2307 at 359.074 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.210 kb on - strand, within GFF2307 at 359.210 kb on - strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.276 kb on - strand, within GFF2307 at 359.276 kb on - strand, within GFF2307 at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.364 kb on - strand at 359.522 kb on + strand, within GFF2308 at 359.522 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.756 kb on + strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.757 kb on - strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.831 kb on + strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.832 kb on - strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.840 kb on + strand, within GFF2308 at 359.841 kb on - strand, within GFF2308 at 359.841 kb on - strand, within GFF2308 at 359.841 kb on - strand, within GFF2308 at 359.841 kb on - strand, within GFF2308 at 359.841 kb on - strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.843 kb on + strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.844 kb on - strand, within GFF2308 at 359.876 kb on + strand, within GFF2308 at 359.876 kb on + strand, within GFF2308 at 359.876 kb on + strand, within GFF2308 at 359.876 kb on + strand, within GFF2308 at 359.876 kb on + strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.877 kb on - strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on + strand, within GFF2308 at 359.903 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.904 kb on - strand, within GFF2308 at 359.909 kb on + strand, within GFF2308 at 359.909 kb on + strand, within GFF2308 at 359.909 kb on + strand, within GFF2308 at 359.909 kb on + strand, within GFF2308 at 359.910 kb on - strand, within GFF2308 at 359.910 kb on - strand, within GFF2308 at 359.910 kb on - strand, within GFF2308 at 359.936 kb on + strand, within GFF2308 at 359.937 kb on - strand, within GFF2308 at 359.937 kb on - strand, within GFF2308 at 359.945 kb on + strand at 359.946 kb on - strand at 360.291 kb on + strand, within GFF2309 at 360.291 kb on + strand, within GFF2309 at 360.291 kb on + strand, within GFF2309 at 360.291 kb on + strand, within GFF2309 at 360.291 kb on + strand, within GFF2309 at 360.291 kb on + strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.292 kb on - strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.690 kb on + strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.691 kb on - strand, within GFF2309 at 360.788 kb on + strand at 360.788 kb on + strand at 360.788 kb on + strand at 360.804 kb on + strand at 360.804 kb on + strand at 360.804 kb on + strand at 360.804 kb on + strand at 360.804 kb on + strand at 360.805 kb on - strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.898 kb on + strand at 360.899 kb on - strand at 360.899 kb on - strand at 360.899 kb on - strand at 360.899 kb on - strand at 360.899 kb on - strand at 360.899 kb on - strand at 360.904 kb on + strand at 360.904 kb on + strand at 360.904 kb on + strand at 360.904 kb on + strand at 360.904 kb on + strand at 360.904 kb on + strand at 360.905 kb on - strand at 360.905 kb on - strand at 360.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 359,060 - GFF2307 0.66 +0.3 359,060 - GFF2307 0.66 +0.1 359,074 + GFF2307 0.67 +0.1 359,146 + GFF2307 0.75 +0.6 359,146 + GFF2307 0.75 -0.6 359,146 + GFF2307 0.75 -1.4 359,146 + GFF2307 0.75 -2.2 359,146 + GFF2307 0.75 +0.1 359,147 - GFF2307 0.75 -1.3 359,147 - GFF2307 0.75 -2.4 359,147 - GFF2307 0.75 -1.4 359,147 - GFF2307 0.75 +1.3 359,147 - GFF2307 0.75 -2.0 359,188 + GFF2307 0.80 -0.5 359,188 + GFF2307 0.80 -0.5 359,188 + GFF2307 0.80 -0.7 359,189 - GFF2307 0.80 -1.2 359,189 - GFF2307 0.80 -0.7 359,189 - GFF2307 0.80 -0.8 359,189 - GFF2307 0.80 -2.2 359,189 - GFF2307 0.80 -1.2 359,209 + GFF2307 0.82 -1.6 359,209 + GFF2307 0.82 -0.1 359,209 + GFF2307 0.82 -0.4 359,209 + GFF2307 0.82 +0.9 359,209 + GFF2307 0.82 -0.1 359,210 - GFF2307 0.82 -0.5 359,210 - GFF2307 0.82 -1.2 359,275 + GFF2307 0.89 -0.9 359,275 + GFF2307 0.89 +0.6 359,275 + GFF2307 0.89 -2.1 359,276 - GFF2307 0.90 +0.0 359,276 - GFF2307 0.90 -2.2 359,359 + +0.5 359,359 + -0.9 359,359 + -1.1 359,359 + +0.5 359,359 + -1.3 359,359 + +0.8 359,359 + -0.1 359,359 + +1.1 359,359 + +0.7 359,359 + +0.5 359,359 + +0.2 359,359 + -0.0 359,359 + +0.3 359,359 + +0.3 359,359 + -1.3 359,360 - +0.6 359,360 - -0.5 359,360 - -0.4 359,360 - -1.1 359,360 - +0.3 359,360 - +0.3 359,360 - +1.5 359,360 - +0.2 359,360 - -0.4 359,360 - -1.1 359,360 - +0.3 359,360 - -0.6 359,360 - -0.2 359,360 - -0.2 359,360 - -0.3 359,364 - -1.6 359,522 + GFF2308 0.22 -2.0 359,522 + GFF2308 0.22 -0.9 359,756 + GFF2308 0.60 -0.2 359,756 + GFF2308 0.60 -0.3 359,756 + GFF2308 0.60 -0.6 359,756 + GFF2308 0.60 -0.3 359,756 + GFF2308 0.60 -1.0 359,756 + GFF2308 0.60 +0.0 359,756 + GFF2308 0.60 -0.7 359,756 + GFF2308 0.60 +1.2 359,756 + GFF2308 0.60 +0.4 359,756 + GFF2308 0.60 -0.4 359,757 - GFF2308 0.60 -2.0 359,757 - GFF2308 0.60 +0.0 359,757 - GFF2308 0.60 +0.6 359,757 - GFF2308 0.60 -1.0 359,757 - GFF2308 0.60 -1.3 359,757 - GFF2308 0.60 -0.2 359,757 - GFF2308 0.60 -0.2 359,757 - GFF2308 0.60 +0.3 359,757 - GFF2308 0.60 -1.1 359,831 + GFF2308 0.72 -1.4 359,831 + GFF2308 0.72 +1.3 359,831 + GFF2308 0.72 +0.3 359,831 + GFF2308 0.72 -0.2 359,831 + GFF2308 0.72 +2.3 359,831 + GFF2308 0.72 -0.6 359,831 + GFF2308 0.72 -0.9 359,831 + GFF2308 0.72 -0.4 359,831 + GFF2308 0.72 +0.6 359,832 - GFF2308 0.72 -0.1 359,832 - GFF2308 0.72 -0.8 359,832 - GFF2308 0.72 -0.1 359,832 - GFF2308 0.72 -0.8 359,832 - GFF2308 0.72 -0.6 359,832 - GFF2308 0.72 -0.1 359,832 - GFF2308 0.72 -0.1 359,832 - GFF2308 0.72 +0.5 359,832 - GFF2308 0.72 +0.6 359,832 - GFF2308 0.72 -0.0 359,832 - GFF2308 0.72 +0.2 359,840 + GFF2308 0.74 +0.1 359,840 + GFF2308 0.74 +0.2 359,840 + GFF2308 0.74 +0.5 359,840 + GFF2308 0.74 -1.1 359,840 + GFF2308 0.74 +2.9 359,840 + GFF2308 0.74 +0.1 359,840 + GFF2308 0.74 +0.7 359,840 + GFF2308 0.74 -0.7 359,840 + GFF2308 0.74 +1.0 359,841 - GFF2308 0.74 -0.4 359,841 - GFF2308 0.74 -1.3 359,841 - GFF2308 0.74 -0.4 359,841 - GFF2308 0.74 -0.1 359,841 - GFF2308 0.74 -0.7 359,843 + GFF2308 0.74 +0.1 359,843 + GFF2308 0.74 +0.1 359,843 + GFF2308 0.74 +0.2 359,843 + GFF2308 0.74 +0.3 359,843 + GFF2308 0.74 -0.9 359,843 + GFF2308 0.74 -0.9 359,844 - GFF2308 0.74 -0.4 359,844 - GFF2308 0.74 +0.2 359,844 - GFF2308 0.74 -0.9 359,844 - GFF2308 0.74 -0.3 359,844 - GFF2308 0.74 -0.3 359,844 - GFF2308 0.74 -0.3 359,844 - GFF2308 0.74 -0.1 359,844 - GFF2308 0.74 +0.4 359,876 + GFF2308 0.80 -0.5 359,876 + GFF2308 0.80 +0.3 359,876 + GFF2308 0.80 +0.2 359,876 + GFF2308 0.80 +0.6 359,876 + GFF2308 0.80 +0.4 359,877 - GFF2308 0.80 +0.8 359,877 - GFF2308 0.80 -0.9 359,877 - GFF2308 0.80 +0.4 359,877 - GFF2308 0.80 +0.3 359,877 - GFF2308 0.80 -0.1 359,877 - GFF2308 0.80 -0.4 359,877 - GFF2308 0.80 -0.8 359,903 + GFF2308 0.84 +0.1 359,903 + GFF2308 0.84 -0.3 359,903 + GFF2308 0.84 -1.0 359,903 + GFF2308 0.84 -0.4 359,903 + GFF2308 0.84 -0.7 359,903 + GFF2308 0.84 +1.9 359,903 + GFF2308 0.84 -0.2 359,903 - GFF2308 0.84 +1.2 359,904 - GFF2308 0.84 +0.7 359,904 - GFF2308 0.84 -0.1 359,904 - GFF2308 0.84 -0.0 359,904 - GFF2308 0.84 +0.7 359,904 - GFF2308 0.84 -0.7 359,904 - GFF2308 0.84 -2.1 359,904 - GFF2308 0.84 -0.7 359,904 - GFF2308 0.84 +0.6 359,904 - GFF2308 0.84 -1.5 359,904 - GFF2308 0.84 +0.2 359,909 + GFF2308 0.85 +0.3 359,909 + GFF2308 0.85 -0.1 359,909 + GFF2308 0.85 +1.3 359,909 + GFF2308 0.85 -0.8 359,910 - GFF2308 0.85 +1.2 359,910 - GFF2308 0.85 +1.7 359,910 - GFF2308 0.85 -0.3 359,936 + GFF2308 0.89 -0.6 359,937 - GFF2308 0.89 -0.4 359,937 - GFF2308 0.89 +0.0 359,945 + -0.4 359,946 - -0.4 360,291 + GFF2309 0.33 -0.3 360,291 + GFF2309 0.33 +0.3 360,291 + GFF2309 0.33 +0.6 360,291 + GFF2309 0.33 +1.0 360,291 + GFF2309 0.33 +0.2 360,291 + GFF2309 0.33 +0.2 360,292 - GFF2309 0.33 -0.5 360,292 - GFF2309 0.33 +0.9 360,292 - GFF2309 0.33 -0.7 360,292 - GFF2309 0.33 -1.7 360,292 - GFF2309 0.33 +0.4 360,292 - GFF2309 0.33 -1.4 360,292 - GFF2309 0.33 +0.5 360,690 + GFF2309 0.82 -0.4 360,690 + GFF2309 0.82 +0.2 360,690 + GFF2309 0.82 -0.1 360,690 + GFF2309 0.82 -0.9 360,690 + GFF2309 0.82 +1.9 360,690 + GFF2309 0.82 +0.3 360,690 + GFF2309 0.82 +0.5 360,690 + GFF2309 0.82 -0.4 360,691 - GFF2309 0.82 +0.6 360,691 - GFF2309 0.82 +0.1 360,691 - GFF2309 0.82 -1.3 360,691 - GFF2309 0.82 -0.1 360,691 - GFF2309 0.82 +0.3 360,691 - GFF2309 0.82 -0.3 360,691 - GFF2309 0.82 +0.6 360,691 - GFF2309 0.82 +1.5 360,788 + +0.1 360,788 + +0.2 360,788 + +1.0 360,804 + -0.2 360,804 + +0.3 360,804 + -0.7 360,804 + +0.6 360,804 + -1.2 360,805 - +0.6 360,898 + -0.3 360,898 + +0.3 360,898 + -0.2 360,898 + +0.9 360,898 + +0.2 360,898 + +0.7 360,898 + +0.2 360,898 + -1.2 360,898 + +0.3 360,899 - -1.5 360,899 - -0.4 360,899 - -0.3 360,899 - +0.2 360,899 - -0.2 360,899 - -0.5 360,904 + -0.0 360,904 + -0.8 360,904 + -1.7 360,904 + -1.2 360,904 + -0.6 360,904 + -1.5 360,905 - -0.6 360,905 - +0.7 360,905 - -0.0
Or see this region's nucleotide sequence