Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1924 and GFF1925 are separated by 60 nucleotides GFF1925 and GFF1926 overlap by 4 nucleotides
GFF1924 - Aliphatic sulfonate monooxygenase family, FMNH2- or F420-dependent, at 604,448 to 605,569
GFF1924
GFF1925 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 605,630 to 606,454
GFF1925
GFF1926 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 606,451 to 607,458
GFF1926
Position (kb) 605
606
607 Strain fitness (log2 ratio) -2
-1
0
1 at 604.781 kb on + strand, within GFF1924 at 604.782 kb on - strand, within GFF1924 at 604.782 kb on - strand, within GFF1924 at 604.847 kb on + strand, within GFF1924 at 604.865 kb on + strand, within GFF1924 at 604.866 kb on - strand, within GFF1924 at 604.866 kb on - strand, within GFF1924 at 604.887 kb on + strand, within GFF1924 at 604.887 kb on + strand, within GFF1924 at 605.000 kb on + strand, within GFF1924 at 605.000 kb on + strand, within GFF1924 at 605.001 kb on - strand, within GFF1924 at 605.045 kb on + strand, within GFF1924 at 605.046 kb on - strand, within GFF1924 at 605.046 kb on - strand, within GFF1924 at 605.252 kb on + strand, within GFF1924 at 605.429 kb on + strand, within GFF1924 at 605.429 kb on + strand, within GFF1924 at 605.432 kb on + strand, within GFF1924 at 605.433 kb on - strand, within GFF1924 at 605.434 kb on - strand, within GFF1924 at 605.496 kb on - strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.619 kb on + strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.620 kb on - strand at 605.702 kb on + strand at 605.702 kb on + strand at 605.703 kb on - strand at 606.044 kb on + strand, within GFF1925 at 606.045 kb on - strand, within GFF1925 at 606.269 kb on + strand, within GFF1925 at 606.269 kb on + strand, within GFF1925 at 606.270 kb on - strand, within GFF1925 at 606.673 kb on + strand, within GFF1926 at 606.674 kb on - strand, within GFF1926 at 606.674 kb on - strand, within GFF1926 at 607.051 kb on + strand, within GFF1926 at 607.051 kb on + strand, within GFF1926 at 607.082 kb on - strand, within GFF1926 at 607.114 kb on + strand, within GFF1926 at 607.114 kb on + strand, within GFF1926 at 607.114 kb on + strand, within GFF1926 at 607.115 kb on - strand, within GFF1926 at 607.115 kb on - strand, within GFF1926 at 607.220 kb on - strand, within GFF1926 at 607.220 kb on - strand, within GFF1926 at 607.265 kb on - strand, within GFF1926 at 607.265 kb on - strand, within GFF1926 at 607.366 kb on + strand at 607.367 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 604,781 + GFF1924 0.30 +0.9 604,782 - GFF1924 0.30 +0.6 604,782 - GFF1924 0.30 -1.3 604,847 + GFF1924 0.36 -0.3 604,865 + GFF1924 0.37 -1.9 604,866 - GFF1924 0.37 +0.3 604,866 - GFF1924 0.37 -0.1 604,887 + GFF1924 0.39 -0.1 604,887 + GFF1924 0.39 -0.4 605,000 + GFF1924 0.49 +0.1 605,000 + GFF1924 0.49 -1.1 605,001 - GFF1924 0.49 -1.1 605,045 + GFF1924 0.53 -0.1 605,046 - GFF1924 0.53 -1.3 605,046 - GFF1924 0.53 +1.3 605,252 + GFF1924 0.72 +0.1 605,429 + GFF1924 0.87 +0.3 605,429 + GFF1924 0.87 -0.3 605,432 + GFF1924 0.88 -0.4 605,433 - GFF1924 0.88 +0.2 605,434 - GFF1924 0.88 -0.7 605,496 - -0.3 605,619 + -1.3 605,619 + +0.2 605,619 + -1.3 605,619 + -0.0 605,619 + +0.1 605,619 + +0.9 605,619 + +0.2 605,620 - -0.6 605,620 - +0.3 605,620 - -0.2 605,620 - +0.5 605,702 + +0.3 605,702 + +0.2 605,703 - +0.3 606,044 + GFF1925 0.50 -0.7 606,045 - GFF1925 0.50 -1.7 606,269 + GFF1925 0.77 -0.4 606,269 + GFF1925 0.77 -0.8 606,270 - GFF1925 0.78 -0.5 606,673 + GFF1926 0.22 -1.7 606,674 - GFF1926 0.22 +0.9 606,674 - GFF1926 0.22 -0.4 607,051 + GFF1926 0.60 +0.2 607,051 + GFF1926 0.60 -0.2 607,082 - GFF1926 0.63 -0.1 607,114 + GFF1926 0.66 -0.3 607,114 + GFF1926 0.66 -0.4 607,114 + GFF1926 0.66 +0.4 607,115 - GFF1926 0.66 +0.2 607,115 - GFF1926 0.66 +0.1 607,220 - GFF1926 0.76 -0.3 607,220 - GFF1926 0.76 +0.8 607,265 - GFF1926 0.81 -2.3 607,265 - GFF1926 0.81 +0.6 607,366 + -0.0 607,367 - -1.5
Or see this region's nucleotide sequence