Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt GFF168 and GFF169 overlap by 4 nucleotides GFF169 and GFF170 overlap by 11 nucleotides GFF170 and GFF171 are separated by 73 nucleotides GFF171 and GFF172 are separated by 74 nucleotides  
        
        GFF168 - MCP methyltransferase, CheR-type, at 199,194 to 200,060 
         
        GFF168 
         
        
        GFF169 - Protein-glutamate methylesterase (EC 3.1.1.61), at 200,057 to 200,659 
         
        GFF169 
         
        
        GFF170 - Two-component system sensor histidine kinase/response regulator hybrid, at 200,649 to 201,872 
         
        GFF170 
         
        
        GFF171 - Histidine kinase, at 201,946 to 202,305 
         
        GFF171 
         
        
        GFF172 - hypothetical protein, at 202,380 to 204,242 
         
        GFF172 
         Position (kb)  
200 
 
201 
 
202 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.722 kb on + strand, within GFF168 at 199.723 kb on - strand, within GFF168 at 199.758 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.773 kb on + strand, within GFF168 at 199.774 kb on - strand, within GFF168 at 199.893 kb on + strand, within GFF168 at 199.894 kb on - strand, within GFF168 at 200.043 kb on + strand at 200.044 kb on - strand at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.321 kb on + strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.334 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.527 kb on - strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.931 kb on + strand, within GFF170 at 200.931 kb on + strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.946 kb on + strand, within GFF170 at 200.947 kb on - strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.149 kb on + strand, within GFF170 at 201.150 kb on - strand, within GFF170 at 201.210 kb on + strand, within GFF170 at 201.211 kb on - strand, within GFF170 at 201.211 kb on - strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.735 kb on + strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.736 kb on - strand, within GFF170 at 201.773 kb on + strand at 201.773 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.870 kb on + strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.871 kb on - strand at 201.882 kb on + strand at 201.882 kb on + strand at 201.883 kb on - strand at 201.927 kb on - strand at 201.927 kb on - strand at 202.032 kb on + strand, within GFF171 at 202.032 kb on + strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.033 kb on - strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.036 kb on + strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.037 kb on - strand, within GFF171 at 202.384 kb on + strand at 202.384 kb on + strand at 202.385 kb on - strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.420 kb on + strand at 202.421 kb on - strand at 202.421 kb on - strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.450 kb on + strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.451 kb on - strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.501 kb on + strand at 202.502 kb on - strand at 202.502 kb on - strand at 202.502 kb on - strand at 202.502 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL             remove   199,710   +    GFF168   0.60  -0.1   199,710   +    GFF168   0.60  -0.4   199,710   +    GFF168   0.60  -3.1   199,722   +    GFF168   0.61  -0.6   199,723   -    GFF168   0.61  -3.7   199,758   +    GFF168   0.65  -2.4   199,761   +    GFF168   0.65  -0.9   199,761   +    GFF168   0.65  -2.2   199,761   +    GFF168   0.65  +0.2   199,761   +    GFF168   0.65  -0.0   199,761   +    GFF168   0.65  -1.6   199,761   +    GFF168   0.65  -3.2   199,761   +    GFF168   0.65  -0.2   199,761   +    GFF168   0.65  -0.2   199,762   -    GFF168   0.66  -1.9   199,762   -    GFF168   0.66  -1.4   199,773   +    GFF168   0.67  -3.5   199,774   -    GFF168   0.67  -1.6   199,893   +    GFF168   0.81  -0.0   199,894   -    GFF168   0.81  -1.6   200,043   +        -0.8   200,044   -        -1.6   200,175   +    GFF169   0.20  -0.5   200,175   +    GFF169   0.20  -2.2   200,175   +    GFF169   0.20  +0.2   200,175   +    GFF169   0.20  -0.8   200,175   +    GFF169   0.20  +0.5   200,175   +    GFF169   0.20  -0.7   200,176   -    GFF169   0.20  -0.6   200,176   -    GFF169   0.20  -1.6   200,176   -    GFF169   0.20  -0.7   200,303   +    GFF169   0.41  -1.0   200,303   +    GFF169   0.41  -0.4   200,303   +    GFF169   0.41  -0.5   200,303   +    GFF169   0.41  +0.4   200,304   -    GFF169   0.41  -1.0   200,304   -    GFF169   0.41  -0.2   200,304   -    GFF169   0.41  -1.1   200,321   +    GFF169   0.44  -0.7   200,322   -    GFF169   0.44  -0.6   200,322   -    GFF169   0.44  -0.8   200,322   -    GFF169   0.44  -2.7   200,334   +    GFF169   0.46  +0.1   200,471   +    GFF169   0.69  -1.8   200,471   +    GFF169   0.69  -0.6   200,471   +    GFF169   0.69  -2.5   200,471   +    GFF169   0.69  -1.4   200,471   +    GFF169   0.69  -1.1   200,471   +    GFF169   0.69  +0.3   200,471   +    GFF169   0.69  +0.1   200,471   +    GFF169   0.69  -1.3   200,472   -    GFF169   0.69  +0.8   200,472   -    GFF169   0.69  -1.4   200,472   -    GFF169   0.69  +0.4   200,526   +    GFF169   0.78  +0.4   200,526   +    GFF169   0.78  -0.9   200,526   +    GFF169   0.78  -1.1   200,527   -    GFF169   0.78  -0.8   200,594   +    GFF169   0.89  -0.5   200,594   +    GFF169   0.89  +0.1   200,594   +    GFF169   0.89  -1.8   200,594   +    GFF169   0.89  -0.9   200,595   -    GFF169   0.89  +0.7   200,595   -    GFF169   0.89  -1.6   200,595   -    GFF169   0.89  -0.7   200,595   -    GFF169   0.89  -1.1   200,931   +    GFF170   0.23  +0.2   200,931   +    GFF170   0.23  -1.2   200,932   -    GFF170   0.23  +0.0   200,932   -    GFF170   0.23  -0.2   200,932   -    GFF170   0.23  +2.4   200,946   +    GFF170   0.24  +0.1   200,947   -    GFF170   0.24  -0.9   201,149   +    GFF170   0.41  -1.4   201,149   +    GFF170   0.41  -1.4   201,149   +    GFF170   0.41  +1.9   201,149   +    GFF170   0.41  +3.0   201,150   -    GFF170   0.41  -0.7   201,210   +    GFF170   0.46  +0.8   201,211   -    GFF170   0.46  +0.1   201,211   -    GFF170   0.46  +0.1   201,735   +    GFF170   0.89  -0.2   201,735   +    GFF170   0.89  -0.5   201,735   +    GFF170   0.89  -0.0   201,735   +    GFF170   0.89  -0.8   201,735   +    GFF170   0.89  -0.3   201,735   +    GFF170   0.89  -0.1   201,735   +    GFF170   0.89  +0.6   201,735   +    GFF170   0.89  -1.6   201,735   +    GFF170   0.89  -0.2   201,735   +    GFF170   0.89  +0.4   201,735   +    GFF170   0.89  -1.1   201,735   +    GFF170   0.89  -2.2   201,735   +    GFF170   0.89  +1.0   201,735   +    GFF170   0.89  -1.2   201,736   -    GFF170   0.89  +0.1   201,736   -    GFF170   0.89  +0.1   201,736   -    GFF170   0.89  -0.5   201,736   -    GFF170   0.89  +1.3   201,736   -    GFF170   0.89  +0.8   201,736   -    GFF170   0.89  +0.5   201,736   -    GFF170   0.89  -0.4   201,736   -    GFF170   0.89  +0.1   201,736   -    GFF170   0.89  -0.4   201,736   -    GFF170   0.89  -0.5   201,773   +        -0.5   201,773   +        -0.2   201,870   +        +1.4   201,870   +        -0.6   201,870   +        +0.1   201,870   +        +0.9   201,870   +        -1.0   201,870   +        +0.1   201,870   +        -0.4   201,870   +        -0.4   201,870   +        +0.1   201,870   +        -0.1   201,870   +        -0.2   201,870   +        +0.0   201,870   +        -1.2   201,870   +        -0.1   201,871   -        -1.5   201,871   -        +0.2   201,871   -        -1.2   201,871   -        -0.8   201,871   -        -0.3   201,871   -        -0.3   201,871   -        -0.4   201,871   -        -0.4   201,871   -        +0.7   201,871   -        +0.7   201,882   +        -0.4   201,882   +        -0.2   201,883   -        -1.3   201,927   -        +0.2   201,927   -        +0.1   202,032   +    GFF171   0.24  -0.8   202,032   +    GFF171   0.24  -0.2   202,033   -    GFF171   0.24  +1.0   202,033   -    GFF171   0.24  +0.1   202,033   -    GFF171   0.24  -1.4   202,033   -    GFF171   0.24  +0.1   202,033   -    GFF171   0.24  -0.4   202,033   -    GFF171   0.24  -0.3   202,036   +    GFF171   0.25  -1.3   202,036   +    GFF171   0.25  +0.4   202,036   +    GFF171   0.25  -0.6   202,036   +    GFF171   0.25  -0.5   202,036   +    GFF171   0.25  +0.1   202,036   +    GFF171   0.25  +0.2   202,036   +    GFF171   0.25  -1.8   202,036   +    GFF171   0.25  +0.4   202,036   +    GFF171   0.25  +0.8   202,036   +    GFF171   0.25  +0.8   202,037   -    GFF171   0.25  -0.7   202,037   -    GFF171   0.25  -0.2   202,037   -    GFF171   0.25  +0.2   202,037   -    GFF171   0.25  +1.0   202,037   -    GFF171   0.25  -0.2   202,384   +        -0.6   202,384   +        -0.6   202,385   -        +0.3   202,420   +        +0.0   202,420   +        -0.1   202,420   +        +0.5   202,420   +        -0.2   202,420   +        -0.5   202,421   -        -0.4   202,421   -        -0.4   202,450   +        -1.2   202,450   +        +1.4   202,450   +        -1.1   202,450   +        +0.7   202,450   +        +0.1   202,450   +        -0.4   202,450   +        -1.1   202,450   +        +0.2   202,450   +        +0.7   202,450   +        -0.6   202,450   +        -0.3   202,450   +        +1.0   202,450   +        +0.2   202,450   +        -0.3   202,450   +        +0.1   202,450   +        -1.2   202,450   +        +0.4   202,451   -        +0.1   202,451   -        +0.1   202,451   -        -1.1   202,451   -        -0.8   202,451   -        +0.6   202,451   -        -0.4   202,451   -        -0.1   202,451   -        -0.6   202,451   -        -0.2   202,451   -        -0.3   202,451   -        +0.4   202,451   -        -1.2   202,451   -        +0.2   202,451   -        +0.4   202,451   -        +1.4   202,501   +        -0.8   202,501   +        -0.7   202,501   +        -0.7   202,501   +        -0.1   202,502   -        -1.0   202,502   -        +0.2   202,502   -        -0.6   202,502   -        +1.2 
 
Or see this region's nucleotide sequence