Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1663 and GFF1664 overlap by 4 nucleotides GFF1664 and GFF1665 overlap by 4 nucleotides GFF1665 and GFF1666 overlap by 4 nucleotides
GFF1663 - Isocitrate lyase (EC 4.1.3.1), at 307,106 to 307,972
GFF1663
GFF1664 - redox proteins related to the succinate dehydrogenases and fumarate reductases, at 307,969 to 309,384
GFF1664
GFF1665 - Nicotinamidase/isochorismatase family protein, at 309,381 to 309,995
GFF1665
GFF1666 - N-methylhydantoinase A (EC 3.5.2.14), at 309,992 to 312,049
GFF1666
Position (kb) 307
308
309
310 Strain fitness (log2 ratio) -1
0
1
2 at 307.116 kb on + strand at 307.117 kb on - strand at 307.230 kb on + strand, within GFF1663 at 307.231 kb on + strand, within GFF1663 at 307.231 kb on - strand, within GFF1663 at 307.231 kb on - strand, within GFF1663 at 307.233 kb on + strand, within GFF1663 at 307.233 kb on + strand, within GFF1663 at 307.458 kb on + strand, within GFF1663 at 307.833 kb on + strand, within GFF1663 at 307.857 kb on + strand, within GFF1663 at 307.858 kb on - strand, within GFF1663 at 307.908 kb on + strand at 307.908 kb on + strand at 307.908 kb on + strand at 307.909 kb on - strand at 307.909 kb on - strand at 307.909 kb on - strand at 307.957 kb on - strand at 308.066 kb on + strand at 308.067 kb on - strand at 308.225 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.437 kb on + strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 308.438 kb on - strand, within GFF1664 at 309.065 kb on + strand, within GFF1664 at 309.065 kb on + strand, within GFF1664 at 309.066 kb on - strand, within GFF1664 at 309.637 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 309.907 kb on + strand, within GFF1665 at 310.318 kb on - strand, within GFF1666
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 307,116 + -0.5 307,117 - -0.6 307,230 + GFF1663 0.14 +1.5 307,231 + GFF1663 0.14 -0.6 307,231 - GFF1663 0.14 +0.0 307,231 - GFF1663 0.14 -1.0 307,233 + GFF1663 0.15 +0.4 307,233 + GFF1663 0.15 -1.3 307,458 + GFF1663 0.41 -1.3 307,833 + GFF1663 0.84 -0.6 307,857 + GFF1663 0.87 +1.1 307,858 - GFF1663 0.87 -0.6 307,908 + -0.7 307,908 + +0.1 307,908 + -0.4 307,909 - +0.4 307,909 - -0.5 307,909 - +1.9 307,957 - -0.6 308,066 + -0.8 308,067 - -0.2 308,225 + GFF1664 0.18 +0.4 308,437 + GFF1664 0.33 -0.7 308,437 + GFF1664 0.33 +0.3 308,437 + GFF1664 0.33 -0.4 308,438 - GFF1664 0.33 -0.7 308,438 - GFF1664 0.33 +1.0 308,438 - GFF1664 0.33 -1.3 308,438 - GFF1664 0.33 -0.4 309,065 + GFF1664 0.77 +0.0 309,065 + GFF1664 0.77 +0.3 309,066 - GFF1664 0.77 -0.8 309,637 + GFF1665 0.42 +0.5 309,907 + GFF1665 0.86 -0.1 309,907 + GFF1665 0.86 -0.0 309,907 + GFF1665 0.86 -0.9 310,318 - GFF1666 0.16 -0.0
Or see this region's nucleotide sequence