Strain Fitness in Variovorax sp. SCN45 around GFF1575

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1574 and GFF1575 overlap by 4 nucleotidesGFF1575 and GFF1576 are separated by 19 nucleotidesGFF1576 and GFF1577 are separated by 19 nucleotides GFF1574 - Alpha-aminoadipate aminotransferase (EC 2.6.1.39) @ Leucine transaminase (EC 2.6.1.6) @ Valine transaminase, at 174,476 to 175,687 GFF1574 GFF1575 - BUG/TctC family periplasmic protein, at 175,684 to 176,655 GFF1575 GFF1576 - 2-pyrone-4,6-dicarboxylic acid hydrolase (EC 3.1.1.57), at 176,675 to 177,592 GFF1576 GFF1577 - hypothetical protein, at 177,612 to 178,052 GFF1577 Position (kb) 175 176 177Strain fitness (log2 ratio) -2 -1 0 1at 174.860 kb on + strand, within GFF1574at 174.861 kb on - strand, within GFF1574at 174.890 kb on + strand, within GFF1574at 174.902 kb on + strand, within GFF1574at 174.978 kb on - strand, within GFF1574at 174.978 kb on - strand, within GFF1574at 174.978 kb on - strand, within GFF1574at 175.208 kb on + strand, within GFF1574at 175.208 kb on + strand, within GFF1574at 175.208 kb on + strand, within GFF1574at 175.208 kb on + strand, within GFF1574at 175.208 kb on + strand, within GFF1574at 175.209 kb on - strand, within GFF1574at 175.209 kb on - strand, within GFF1574at 175.209 kb on - strand, within GFF1574at 175.209 kb on - strand, within GFF1574at 175.283 kb on + strand, within GFF1574at 176.149 kb on + strand, within GFF1575at 176.149 kb on + strand, within GFF1575at 176.149 kb on + strand, within GFF1575at 176.149 kb on + strand, within GFF1575at 176.150 kb on - strand, within GFF1575at 176.150 kb on - strand, within GFF1575at 176.606 kb on - strandat 176.606 kb on - strandat 176.826 kb on - strand, within GFF1576at 176.843 kb on + strand, within GFF1576at 176.843 kb on + strand, within GFF1576at 176.844 kb on - strand, within GFF1576at 176.987 kb on + strand, within GFF1576at 176.987 kb on + strand, within GFF1576at 176.988 kb on - strand, within GFF1576at 177.228 kb on - strand, within GFF1576at 177.228 kb on - strand, within GFF1576at 177.349 kb on + strand, within GFF1576at 177.350 kb on - strand, within GFF1576at 177.579 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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174,860 + GFF1574 0.32 +0.5
174,861 - GFF1574 0.32 -0.3
174,890 + GFF1574 0.34 -0.5
174,902 + GFF1574 0.35 -0.7
174,978 - GFF1574 0.41 -0.2
174,978 - GFF1574 0.41 -0.1
174,978 - GFF1574 0.41 -0.4
175,208 + GFF1574 0.60 -0.2
175,208 + GFF1574 0.60 -0.3
175,208 + GFF1574 0.60 +0.1
175,208 + GFF1574 0.60 -0.2
175,208 + GFF1574 0.60 -0.7
175,209 - GFF1574 0.60 +1.1
175,209 - GFF1574 0.60 -0.3
175,209 - GFF1574 0.60 +0.0
175,209 - GFF1574 0.60 +0.0
175,283 + GFF1574 0.67 -0.7
176,149 + GFF1575 0.48 -0.8
176,149 + GFF1575 0.48 +0.3
176,149 + GFF1575 0.48 -0.0
176,149 + GFF1575 0.48 -0.2
176,150 - GFF1575 0.48 +0.7
176,150 - GFF1575 0.48 +0.3
176,606 - +0.9
176,606 - -0.6
176,826 - GFF1576 0.16 -0.9
176,843 + GFF1576 0.18 -0.4
176,843 + GFF1576 0.18 -0.9
176,844 - GFF1576 0.18 -0.2
176,987 + GFF1576 0.34 +1.1
176,987 + GFF1576 0.34 -1.2
176,988 - GFF1576 0.34 -1.3
177,228 - GFF1576 0.60 -0.3
177,228 - GFF1576 0.60 -0.7
177,349 + GFF1576 0.73 -2.0
177,350 - GFF1576 0.74 +0.1
177,579 - -0.5

Or see this region's nucleotide sequence