Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1527 and GFF1528 are separated by 6 nucleotides GFF1528 and GFF1529 overlap by 7 nucleotides GFF1529 and GFF1530 are separated by 77 nucleotides
GFF1527 - Ferrichrome-iron receptor, at 123,139 to 125,598
GFF1527
GFF1528 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 125,605 to 126,858
GFF1528
GFF1529 - Transcriptional regulator, AcrR family, at 126,852 to 127,421
GFF1529
GFF1530 - Nucleoside-diphosphate-sugar epimerases, at 127,499 to 128,392
GFF1530
Position (kb)
125
126
127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 124.705 kb on + strand, within GFF1527 at 124.705 kb on + strand, within GFF1527 at 124.705 kb on + strand, within GFF1527 at 124.706 kb on - strand, within GFF1527 at 124.706 kb on - strand, within GFF1527 at 124.706 kb on - strand, within GFF1527 at 124.706 kb on - strand, within GFF1527 at 124.855 kb on + strand, within GFF1527 at 124.855 kb on + strand, within GFF1527 at 124.856 kb on - strand, within GFF1527 at 124.984 kb on + strand, within GFF1527 at 125.026 kb on + strand, within GFF1527 at 125.027 kb on - strand, within GFF1527 at 125.027 kb on - strand, within GFF1527 at 125.167 kb on + strand, within GFF1527 at 125.167 kb on + strand, within GFF1527 at 125.167 kb on + strand, within GFF1527 at 125.167 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.173 kb on + strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.174 kb on - strand, within GFF1527 at 125.281 kb on + strand, within GFF1527 at 125.281 kb on + strand, within GFF1527 at 125.281 kb on + strand, within GFF1527 at 125.281 kb on + strand, within GFF1527 at 125.282 kb on - strand, within GFF1527 at 125.282 kb on - strand, within GFF1527 at 125.339 kb on - strand, within GFF1527 at 125.339 kb on - strand, within GFF1527 at 125.339 kb on - strand, within GFF1527 at 125.359 kb on + strand at 125.420 kb on - strand at 125.420 kb on - strand at 125.420 kb on - strand at 125.420 kb on - strand at 125.428 kb on + strand at 125.428 kb on + strand at 125.428 kb on + strand at 125.429 kb on - strand at 125.446 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.507 kb on - strand at 125.507 kb on - strand at 125.549 kb on - strand at 125.549 kb on - strand at 125.549 kb on - strand at 125.551 kb on + strand at 125.551 kb on + strand at 125.551 kb on + strand at 125.551 kb on + strand at 125.552 kb on - strand at 125.587 kb on + strand at 125.587 kb on + strand at 125.587 kb on + strand at 125.587 kb on + strand at 125.951 kb on - strand, within GFF1528 at 125.951 kb on - strand, within GFF1528 at 125.951 kb on - strand, within GFF1528 at 126.025 kb on + strand, within GFF1528 at 126.026 kb on - strand, within GFF1528 at 126.037 kb on + strand, within GFF1528 at 126.037 kb on + strand, within GFF1528 at 126.038 kb on - strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.050 kb on + strand, within GFF1528 at 126.051 kb on - strand, within GFF1528 at 126.051 kb on - strand, within GFF1528 at 126.051 kb on - strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.136 kb on + strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.137 kb on - strand, within GFF1528 at 126.138 kb on + strand, within GFF1528 at 126.138 kb on + strand, within GFF1528 at 126.138 kb on + strand, within GFF1528 at 126.138 kb on + strand, within GFF1528 at 126.139 kb on - strand, within GFF1528 at 126.258 kb on - strand, within GFF1528 at 126.384 kb on - strand, within GFF1528 at 126.493 kb on + strand, within GFF1528 at 126.494 kb on - strand, within GFF1528 at 126.506 kb on - strand, within GFF1528 at 126.506 kb on - strand, within GFF1528 at 126.506 kb on - strand, within GFF1528 at 126.514 kb on + strand, within GFF1528 at 126.515 kb on - strand, within GFF1528 at 126.580 kb on + strand, within GFF1528 at 126.580 kb on + strand, within GFF1528 at 126.581 kb on - strand, within GFF1528 at 126.581 kb on - strand, within GFF1528 at 126.764 kb on + strand at 126.854 kb on - strand at 127.283 kb on - strand, within GFF1529 at 127.456 kb on + strand at 127.457 kb on - strand at 127.463 kb on - strand at 127.463 kb on - strand at 127.578 kb on + strand at 127.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 124,705 + GFF1527 0.64 +0.1 124,705 + GFF1527 0.64 -0.8 124,705 + GFF1527 0.64 +1.3 124,706 - GFF1527 0.64 -0.2 124,706 - GFF1527 0.64 -0.4 124,706 - GFF1527 0.64 -2.3 124,706 - GFF1527 0.64 -0.1 124,855 + GFF1527 0.70 -1.8 124,855 + GFF1527 0.70 -0.7 124,856 - GFF1527 0.70 -0.1 124,984 + GFF1527 0.75 +1.3 125,026 + GFF1527 0.77 -0.8 125,027 - GFF1527 0.77 -1.1 125,027 - GFF1527 0.77 -0.7 125,167 + GFF1527 0.82 +0.6 125,167 + GFF1527 0.82 -0.4 125,167 + GFF1527 0.82 +0.3 125,167 + GFF1527 0.82 +1.1 125,173 + GFF1527 0.83 +0.3 125,173 + GFF1527 0.83 -0.2 125,173 + GFF1527 0.83 +0.1 125,173 + GFF1527 0.83 +0.3 125,173 + GFF1527 0.83 -0.8 125,173 + GFF1527 0.83 -1.2 125,173 + GFF1527 0.83 -0.1 125,173 + GFF1527 0.83 -0.4 125,173 + GFF1527 0.83 +1.9 125,173 + GFF1527 0.83 +0.9 125,173 + GFF1527 0.83 -0.8 125,173 + GFF1527 0.83 +0.2 125,173 + GFF1527 0.83 -0.5 125,173 + GFF1527 0.83 -0.1 125,173 + GFF1527 0.83 -0.3 125,173 + GFF1527 0.83 -1.3 125,173 + GFF1527 0.83 -0.1 125,174 - GFF1527 0.83 +0.3 125,174 - GFF1527 0.83 +1.1 125,174 - GFF1527 0.83 +0.1 125,174 - GFF1527 0.83 -0.5 125,174 - GFF1527 0.83 -0.7 125,174 - GFF1527 0.83 +0.0 125,174 - GFF1527 0.83 +0.9 125,174 - GFF1527 0.83 -1.0 125,281 + GFF1527 0.87 -0.7 125,281 + GFF1527 0.87 -2.4 125,281 + GFF1527 0.87 -1.5 125,281 + GFF1527 0.87 +0.3 125,282 - GFF1527 0.87 +0.7 125,282 - GFF1527 0.87 -0.4 125,339 - GFF1527 0.89 +0.7 125,339 - GFF1527 0.89 -0.4 125,339 - GFF1527 0.89 +1.3 125,359 + -0.6 125,420 - +0.3 125,420 - +0.5 125,420 - -0.5 125,420 - -0.1 125,428 + -0.7 125,428 + -0.2 125,428 + +0.8 125,429 - +1.1 125,446 + +0.2 125,506 + -0.7 125,506 + +2.3 125,506 + +2.3 125,507 - -0.5 125,507 - -0.7 125,549 - -1.2 125,549 - -0.2 125,549 - +0.1 125,551 + -0.3 125,551 + -0.8 125,551 + -0.4 125,551 + -0.4 125,552 - +0.2 125,587 + -0.6 125,587 + -0.4 125,587 + +0.0 125,587 + -1.4 125,951 - GFF1528 0.28 +0.8 125,951 - GFF1528 0.28 +0.6 125,951 - GFF1528 0.28 -0.3 126,025 + GFF1528 0.33 +1.6 126,026 - GFF1528 0.34 +0.3 126,037 + GFF1528 0.34 -0.4 126,037 + GFF1528 0.34 -0.4 126,038 - GFF1528 0.35 +0.5 126,050 + GFF1528 0.35 +0.3 126,050 + GFF1528 0.35 +0.2 126,050 + GFF1528 0.35 -0.3 126,050 + GFF1528 0.35 +0.3 126,050 + GFF1528 0.35 +0.4 126,050 + GFF1528 0.35 -0.1 126,051 - GFF1528 0.36 -0.4 126,051 - GFF1528 0.36 -0.4 126,051 - GFF1528 0.36 -0.3 126,136 + GFF1528 0.42 -0.3 126,136 + GFF1528 0.42 -0.6 126,136 + GFF1528 0.42 -0.3 126,136 + GFF1528 0.42 +2.1 126,136 + GFF1528 0.42 +1.1 126,136 + GFF1528 0.42 +0.2 126,137 - GFF1528 0.42 +0.2 126,137 - GFF1528 0.42 +0.1 126,137 - GFF1528 0.42 -0.5 126,137 - GFF1528 0.42 -1.5 126,137 - GFF1528 0.42 +0.8 126,137 - GFF1528 0.42 -0.2 126,138 + GFF1528 0.43 -0.1 126,138 + GFF1528 0.43 +0.0 126,138 + GFF1528 0.43 -0.3 126,138 + GFF1528 0.43 -0.4 126,139 - GFF1528 0.43 -0.8 126,258 - GFF1528 0.52 +0.6 126,384 - GFF1528 0.62 +1.1 126,493 + GFF1528 0.71 -0.9 126,494 - GFF1528 0.71 +1.1 126,506 - GFF1528 0.72 -0.1 126,506 - GFF1528 0.72 -0.1 126,506 - GFF1528 0.72 +0.7 126,514 + GFF1528 0.72 -0.2 126,515 - GFF1528 0.73 -0.5 126,580 + GFF1528 0.78 +0.6 126,580 + GFF1528 0.78 +1.0 126,581 - GFF1528 0.78 -0.2 126,581 - GFF1528 0.78 +0.3 126,764 + -0.9 126,854 - -0.0 127,283 - GFF1529 0.76 -1.3 127,456 + +0.0 127,457 - +0.1 127,463 - -0.5 127,463 - -0.3 127,578 + +0.6 127,579 - -1.4
Or see this region's nucleotide sequence