Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1508 and GFF1509 overlap by 4 nucleotides GFF1509 and GFF1510 overlap by 4 nucleotides GFF1510 and GFF1511 are separated by 8 nucleotides
GFF1508 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 101,440 to 102,339
GFF1508
GFF1509 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 102,336 to 103,310
GFF1509
GFF1510 - Oxidoreductase, at 103,307 to 104,797
GFF1510
GFF1511 - Transcriptional regulator, LysR family, at 104,806 to 105,795
GFF1511
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1
2 at 102.321 kb on - strand at 102.321 kb on - strand at 102.379 kb on + strand at 102.380 kb on - strand at 102.380 kb on - strand at 102.380 kb on - strand at 102.436 kb on + strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.437 kb on - strand, within GFF1509 at 102.655 kb on + strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 102.656 kb on - strand, within GFF1509 at 103.120 kb on + strand, within GFF1509 at 103.351 kb on - strand at 103.404 kb on + strand at 103.404 kb on + strand at 103.405 kb on - strand at 103.542 kb on + strand, within GFF1510 at 103.542 kb on + strand, within GFF1510 at 103.543 kb on - strand, within GFF1510 at 103.543 kb on - strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.923 kb on + strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 103.924 kb on - strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.268 kb on + strand, within GFF1510 at 104.269 kb on - strand, within GFF1510 at 104.397 kb on + strand, within GFF1510 at 104.397 kb on + strand, within GFF1510 at 104.592 kb on + strand, within GFF1510 at 104.592 kb on + strand, within GFF1510 at 104.592 kb on + strand, within GFF1510 at 104.592 kb on + strand, within GFF1510 at 104.593 kb on - strand, within GFF1510 at 104.593 kb on - strand, within GFF1510 at 104.850 kb on - strand at 105.004 kb on - strand, within GFF1511 at 105.602 kb on + strand, within GFF1511 at 105.602 kb on + strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.603 kb on - strand, within GFF1511 at 105.663 kb on - strand, within GFF1511 at 105.666 kb on + strand, within GFF1511
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 102,321 - -1.6 102,321 - +0.1 102,379 + -0.1 102,380 - -0.3 102,380 - -0.5 102,380 - +0.1 102,436 + GFF1509 0.10 -0.1 102,437 - GFF1509 0.10 -2.6 102,437 - GFF1509 0.10 +0.0 102,437 - GFF1509 0.10 -0.9 102,655 + GFF1509 0.33 +0.7 102,656 - GFF1509 0.33 +0.3 102,656 - GFF1509 0.33 +1.3 102,656 - GFF1509 0.33 -0.5 103,120 + GFF1509 0.80 -0.5 103,351 - +0.3 103,404 + +0.3 103,404 + +0.1 103,405 - -0.2 103,542 + GFF1510 0.16 +0.1 103,542 + GFF1510 0.16 -0.7 103,543 - GFF1510 0.16 +0.1 103,543 - GFF1510 0.16 -0.3 103,923 + GFF1510 0.41 -0.5 103,923 + GFF1510 0.41 +0.3 103,923 + GFF1510 0.41 -0.3 103,923 + GFF1510 0.41 +0.4 103,923 + GFF1510 0.41 +0.4 103,923 + GFF1510 0.41 +0.8 103,923 + GFF1510 0.41 -0.9 103,924 - GFF1510 0.41 -0.5 103,924 - GFF1510 0.41 +0.7 103,924 - GFF1510 0.41 -0.6 103,924 - GFF1510 0.41 +1.9 104,268 + GFF1510 0.64 -1.4 104,268 + GFF1510 0.64 -0.2 104,268 + GFF1510 0.64 +1.3 104,268 + GFF1510 0.64 -0.3 104,269 - GFF1510 0.65 +0.7 104,397 + GFF1510 0.73 +0.1 104,397 + GFF1510 0.73 -0.5 104,592 + GFF1510 0.86 +1.5 104,592 + GFF1510 0.86 +0.3 104,592 + GFF1510 0.86 -1.3 104,592 + GFF1510 0.86 -1.1 104,593 - GFF1510 0.86 +0.5 104,593 - GFF1510 0.86 -0.4 104,850 - +0.0 105,004 - GFF1511 0.20 -1.2 105,602 + GFF1511 0.80 +0.1 105,602 + GFF1511 0.80 -0.7 105,603 - GFF1511 0.81 +0.5 105,603 - GFF1511 0.81 -0.4 105,603 - GFF1511 0.81 +0.6 105,603 - GFF1511 0.81 -0.7 105,603 - GFF1511 0.81 +0.2 105,603 - GFF1511 0.81 -0.5 105,603 - GFF1511 0.81 -0.7 105,663 - GFF1511 0.87 -0.5 105,666 + GFF1511 0.87 -0.6
Or see this region's nucleotide sequence