Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1326 and GFF1327 are separated by 139 nucleotides GFF1327 and GFF1328 overlap by 4 nucleotides GFF1328 and GFF1329 are separated by 10 nucleotides GFF1329 and GFF1330 overlap by 4 nucleotides
GFF1326 - Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8), at 666,570 to 667,355
GFF1326
GFF1327 - Cell division protein BolA, at 667,495 to 667,788
GFF1327
GFF1328 - Intracellular septation protein IspA, at 667,785 to 668,327
GFF1328
GFF1329 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 668,338 to 668,754
GFF1329
GFF1330 - UPF0061 protein YdiU, at 668,751 to 670,232
GFF1330
Position (kb)
667
668
669 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.991 kb on + strand, within GFF1326 at 666.992 kb on - strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.364 kb on + strand at 667.365 kb on - strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.392 kb on - strand at 667.392 kb on - strand at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.767 kb on + strand at 667.768 kb on - strand at 667.773 kb on + strand at 667.774 kb on - strand at 667.774 kb on - strand at 667.812 kb on - strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.740 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.807 kb on - strand at 668.807 kb on - strand at 668.831 kb on - strand at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.109 kb on + strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.320 kb on - strand, within GFF1330
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 666,955 + GFF1326 0.49 +0.5 666,955 + GFF1326 0.49 +0.3 666,955 + GFF1326 0.49 -0.1 666,955 + GFF1326 0.49 -0.5 666,955 + GFF1326 0.49 +1.1 666,955 + GFF1326 0.49 -1.7 666,955 + GFF1326 0.49 -0.4 666,955 + GFF1326 0.49 +0.0 666,955 + GFF1326 0.49 -0.5 666,955 + GFF1326 0.49 -0.1 666,956 - GFF1326 0.49 +0.4 666,956 - GFF1326 0.49 -1.9 666,956 - GFF1326 0.49 +0.3 666,956 - GFF1326 0.49 +1.9 666,956 - GFF1326 0.49 -1.5 666,956 - GFF1326 0.49 -0.8 666,956 - GFF1326 0.49 +1.5 666,956 - GFF1326 0.49 -0.3 666,991 + GFF1326 0.54 +0.2 666,992 - GFF1326 0.54 +1.9 667,036 + GFF1326 0.59 -0.7 667,036 + GFF1326 0.59 -0.6 667,036 + GFF1326 0.59 +0.0 667,036 + GFF1326 0.59 -0.3 667,036 + GFF1326 0.59 +0.5 667,037 - GFF1326 0.59 +0.5 667,037 - GFF1326 0.59 -0.0 667,037 - GFF1326 0.59 -0.6 667,037 - GFF1326 0.59 +1.5 667,037 - GFF1326 0.59 -0.9 667,037 - GFF1326 0.59 -0.5 667,037 - GFF1326 0.59 +0.7 667,037 - GFF1326 0.59 -1.0 667,037 - GFF1326 0.59 -0.5 667,037 - GFF1326 0.59 +0.3 667,096 + GFF1326 0.67 -0.8 667,096 + GFF1326 0.67 +0.7 667,096 + GFF1326 0.67 -0.6 667,097 - GFF1326 0.67 +0.1 667,097 - GFF1326 0.67 -0.6 667,097 - GFF1326 0.67 -0.4 667,097 - GFF1326 0.67 -1.0 667,364 + -0.1 667,365 - +0.3 667,391 + +0.7 667,391 + +1.9 667,391 + +0.6 667,391 + -0.7 667,391 + -1.8 667,392 - +0.2 667,392 - +1.5 667,556 + GFF1327 0.21 +0.9 667,556 + GFF1327 0.21 +0.9 667,556 + GFF1327 0.21 -0.7 667,556 + GFF1327 0.21 -1.3 667,556 + GFF1327 0.21 +0.5 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 -0.2 667,556 + GFF1327 0.21 +0.0 667,556 + GFF1327 0.21 -0.1 667,556 + GFF1327 0.21 +0.0 667,556 + GFF1327 0.21 -1.1 667,556 + GFF1327 0.21 +0.0 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 -0.2 667,556 + GFF1327 0.21 -0.2 667,556 + GFF1327 0.21 -1.3 667,556 + GFF1327 0.21 -0.4 667,556 + GFF1327 0.21 +0.1 667,557 - GFF1327 0.21 +0.5 667,557 - GFF1327 0.21 +0.4 667,557 - GFF1327 0.21 -0.5 667,557 - GFF1327 0.21 -1.5 667,557 - GFF1327 0.21 +0.4 667,557 - GFF1327 0.21 -0.4 667,557 - GFF1327 0.21 +0.6 667,557 - GFF1327 0.21 -0.2 667,557 - GFF1327 0.21 +1.0 667,557 - GFF1327 0.21 -0.6 667,557 - GFF1327 0.21 +0.5 667,557 - GFF1327 0.21 +0.5 667,557 - GFF1327 0.21 -1.1 667,557 - GFF1327 0.21 -0.4 667,557 - GFF1327 0.21 -1.2 667,767 + +0.3 667,768 - -0.7 667,773 + -0.7 667,774 - -1.3 667,774 - -0.7 667,812 - -0.4 667,828 + +0.4 667,828 + -0.5 667,828 + +0.3 667,828 + -0.5 667,828 + +0.0 667,828 + +0.0 667,828 + -2.1 667,828 + -2.4 667,829 - +1.3 667,829 - +0.8 667,829 - +0.2 667,829 - -0.3 667,829 - -0.8 667,829 - -0.2 667,829 - -1.0 667,829 - -0.1 667,829 - -0.5 667,829 - -0.3 667,829 - +0.1 667,829 - -0.3 668,384 + GFF1329 0.11 +0.9 668,384 + GFF1329 0.11 -0.8 668,384 + GFF1329 0.11 -0.1 668,385 - GFF1329 0.11 -0.0 668,385 - GFF1329 0.11 +1.2 668,385 - GFF1329 0.11 -0.9 668,385 - GFF1329 0.11 -1.1 668,385 - GFF1329 0.11 -0.2 668,385 - GFF1329 0.11 +0.6 668,603 + GFF1329 0.64 -0.0 668,603 + GFF1329 0.64 -0.5 668,603 + GFF1329 0.64 -0.5 668,603 + GFF1329 0.64 -0.2 668,603 + GFF1329 0.64 -1.1 668,603 + GFF1329 0.64 -0.8 668,604 - GFF1329 0.64 -1.2 668,604 - GFF1329 0.64 -1.8 668,604 - GFF1329 0.64 -0.2 668,604 - GFF1329 0.64 +0.9 668,604 - GFF1329 0.64 -0.1 668,604 - GFF1329 0.64 -1.2 668,604 - GFF1329 0.64 -0.6 668,604 - GFF1329 0.64 -1.6 668,604 - GFF1329 0.64 -0.4 668,630 + GFF1329 0.70 -0.3 668,630 + GFF1329 0.70 -0.1 668,630 + GFF1329 0.70 +0.1 668,630 + GFF1329 0.70 -0.8 668,630 + GFF1329 0.70 -0.3 668,631 - GFF1329 0.70 -0.6 668,631 - GFF1329 0.70 +1.2 668,631 - GFF1329 0.70 -1.4 668,740 + +0.5 668,806 + +0.8 668,806 + -0.4 668,806 + +0.4 668,806 + -1.6 668,806 + -1.1 668,806 + +0.1 668,806 + -0.5 668,807 - -1.7 668,807 - -0.1 668,831 - -0.4 669,079 + GFF1330 0.22 +0.1 669,079 + GFF1330 0.22 -0.1 669,079 + GFF1330 0.22 -0.4 669,079 + GFF1330 0.22 -1.9 669,079 + GFF1330 0.22 -0.5 669,079 + GFF1330 0.22 -0.1 669,079 + GFF1330 0.22 -0.8 669,079 + GFF1330 0.22 +0.0 669,080 - GFF1330 0.22 -0.4 669,080 - GFF1330 0.22 -0.1 669,080 - GFF1330 0.22 +1.5 669,080 - GFF1330 0.22 -1.1 669,080 - GFF1330 0.22 -0.7 669,080 - GFF1330 0.22 -0.4 669,109 + GFF1330 0.24 -0.5 669,110 - GFF1330 0.24 -0.9 669,110 - GFF1330 0.24 +0.1 669,110 - GFF1330 0.24 -0.6 669,238 + GFF1330 0.33 +0.1 669,238 + GFF1330 0.33 -0.5 669,238 + GFF1330 0.33 +0.8 669,239 - GFF1330 0.33 +0.4 669,239 - GFF1330 0.33 +0.9 669,239 - GFF1330 0.33 +0.4 669,239 - GFF1330 0.33 -0.6 669,239 - GFF1330 0.33 -0.6 669,239 - GFF1330 0.33 -1.5 669,262 - GFF1330 0.34 -0.3 669,262 - GFF1330 0.34 +0.1 669,262 - GFF1330 0.34 +0.6 669,319 + GFF1330 0.38 -1.0 669,319 + GFF1330 0.38 -0.4 669,320 - GFF1330 0.38 -2.0
Or see this region's nucleotide sequence