Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF120 and GFF121 are separated by 281 nucleotides GFF121 and GFF122 overlap by 4 nucleotides GFF122 and GFF123 overlap by 8 nucleotides
GFF120 - Two-component transcriptional response regulator, LuxR family, at 148,732 to 149,274
GFF120
GFF121 - Uncharacterized MFS-type transporter, at 149,556 to 150,818
GFF121
GFF122 - Dihydroorotate dehydrogenase electron transfer subunit (EC 1.3.3.1), at 150,815 to 151,690
GFF122
GFF123 - Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14), at 151,683 to 152,633
GFF123
Position (kb) 149
150
151 Strain fitness (log2 ratio) -1
0
1 at 148.583 kb on + strand at 148.584 kb on - strand at 148.584 kb on - strand at 148.584 kb on - strand at 149.635 kb on - strand at 149.829 kb on + strand, within GFF121 at 149.829 kb on + strand, within GFF121 at 149.829 kb on + strand, within GFF121 at 149.829 kb on + strand, within GFF121 at 149.830 kb on - strand, within GFF121 at 150.027 kb on + strand, within GFF121 at 150.027 kb on + strand, within GFF121 at 150.310 kb on - strand, within GFF121 at 150.507 kb on + strand, within GFF121 at 150.508 kb on - strand, within GFF121 at 150.508 kb on - strand, within GFF121 at 150.753 kb on + strand at 150.753 kb on + strand at 150.753 kb on + strand at 150.754 kb on - strand at 150.754 kb on - strand at 150.995 kb on + strand, within GFF122 at 151.058 kb on + strand, within GFF122 at 151.059 kb on - strand, within GFF122 at 151.376 kb on + strand, within GFF122 at 151.460 kb on + strand, within GFF122 at 151.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 148,583 + +0.0 148,584 - -0.6 148,584 - +0.4 148,584 - +0.4 149,635 - +0.5 149,829 + GFF121 0.22 +0.1 149,829 + GFF121 0.22 +0.3 149,829 + GFF121 0.22 -0.3 149,829 + GFF121 0.22 +0.3 149,830 - GFF121 0.22 -0.2 150,027 + GFF121 0.37 -0.9 150,027 + GFF121 0.37 -0.8 150,310 - GFF121 0.60 +0.7 150,507 + GFF121 0.75 +0.6 150,508 - GFF121 0.75 -0.3 150,508 - GFF121 0.75 +0.7 150,753 + -0.4 150,753 + -1.0 150,753 + +0.8 150,754 - -0.2 150,754 - -0.6 150,995 + GFF122 0.21 +0.5 151,058 + GFF122 0.28 -0.2 151,059 - GFF122 0.28 -0.6 151,376 + GFF122 0.64 -0.0 151,460 + GFF122 0.74 +0.1 151,764 + -0.5
Or see this region's nucleotide sequence