Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1196 and GFF1197 are separated by 84 nucleotides GFF1197 and GFF1198 overlap by 14 nucleotides GFF1198 and GFF1199 are separated by 85 nucleotides
GFF1196 - BUG/TctC family periplasmic protein, at 514,572 to 515,579
GFF1196
GFF1197 - Transcriptional regulator, LysR family, at 515,664 to 516,599
GFF1197
GFF1198 - no description, at 516,586 to 517,332
GFF1198
GFF1199 - Uncharacterized protein similar to glutamate-1-semialdehyde aminotransferase, at 517,418 to 518,728
GFF1199
Position (kb) 515
516
517 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 514.780 kb on + strand, within GFF1196 at 514.780 kb on + strand, within GFF1196 at 514.780 kb on + strand, within GFF1196 at 514.781 kb on - strand, within GFF1196 at 514.993 kb on + strand, within GFF1196 at 514.993 kb on + strand, within GFF1196 at 514.993 kb on + strand, within GFF1196 at 514.993 kb on + strand, within GFF1196 at 514.994 kb on - strand, within GFF1196 at 515.081 kb on - strand, within GFF1196 at 515.081 kb on - strand, within GFF1196 at 515.081 kb on - strand, within GFF1196 at 515.081 kb on - strand, within GFF1196 at 515.108 kb on - strand, within GFF1196 at 515.108 kb on - strand, within GFF1196 at 515.215 kb on + strand, within GFF1196 at 515.216 kb on - strand, within GFF1196 at 515.216 kb on - strand, within GFF1196 at 515.216 kb on - strand, within GFF1196 at 515.282 kb on - strand, within GFF1196 at 515.393 kb on - strand, within GFF1196 at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.599 kb on + strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.600 kb on - strand at 515.656 kb on + strand at 515.656 kb on + strand at 515.657 kb on - strand at 515.657 kb on - strand at 515.663 kb on + strand at 515.663 kb on + strand at 515.663 kb on + strand at 515.663 kb on + strand at 515.663 kb on + strand at 515.663 kb on + strand at 515.664 kb on - strand at 515.664 kb on - strand at 515.664 kb on - strand at 515.664 kb on - strand at 515.664 kb on - strand at 515.664 kb on - strand at 515.988 kb on + strand, within GFF1197 at 516.058 kb on - strand, within GFF1197 at 516.058 kb on - strand, within GFF1197 at 516.058 kb on - strand, within GFF1197 at 516.058 kb on - strand, within GFF1197 at 516.058 kb on - strand, within GFF1197 at 516.171 kb on + strand, within GFF1197 at 516.171 kb on + strand, within GFF1197 at 516.171 kb on + strand, within GFF1197 at 516.171 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.183 kb on + strand, within GFF1197 at 516.184 kb on - strand, within GFF1197 at 516.184 kb on - strand, within GFF1197 at 516.184 kb on - strand, within GFF1197 at 516.184 kb on - strand, within GFF1197 at 516.588 kb on - strand at 516.640 kb on + strand at 516.640 kb on + strand at 516.640 kb on + strand at 516.640 kb on + strand at 516.640 kb on + strand at 516.640 kb on + strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.641 kb on - strand at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.872 kb on + strand, within GFF1198 at 516.873 kb on - strand, within GFF1198 at 516.873 kb on - strand, within GFF1198 at 516.873 kb on - strand, within GFF1198 at 516.873 kb on - strand, within GFF1198 at 516.873 kb on - strand, within GFF1198 at 517.387 kb on - strand at 517.457 kb on + strand at 517.457 kb on + strand at 517.457 kb on + strand at 517.458 kb on - strand at 517.517 kb on + strand at 517.517 kb on + strand at 517.517 kb on + strand at 517.517 kb on + strand at 517.517 kb on + strand at 517.518 kb on - strand at 517.518 kb on - strand at 517.518 kb on - strand at 517.518 kb on - strand at 517.519 kb on + strand at 517.519 kb on + strand at 517.519 kb on + strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.520 kb on - strand at 517.553 kb on + strand, within GFF1199 at 517.553 kb on + strand, within GFF1199 at 517.554 kb on - strand, within GFF1199 at 517.554 kb on - strand, within GFF1199
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 514,780 + GFF1196 0.21 -0.5 514,780 + GFF1196 0.21 -0.5 514,780 + GFF1196 0.21 -1.5 514,781 - GFF1196 0.21 -0.7 514,993 + GFF1196 0.42 -0.5 514,993 + GFF1196 0.42 +0.4 514,993 + GFF1196 0.42 +0.5 514,993 + GFF1196 0.42 -0.3 514,994 - GFF1196 0.42 +0.5 515,081 - GFF1196 0.50 -0.3 515,081 - GFF1196 0.50 -1.7 515,081 - GFF1196 0.50 +0.3 515,081 - GFF1196 0.50 -0.6 515,108 - GFF1196 0.53 +0.1 515,108 - GFF1196 0.53 +0.9 515,215 + GFF1196 0.64 -0.3 515,216 - GFF1196 0.64 -0.6 515,216 - GFF1196 0.64 +0.1 515,216 - GFF1196 0.64 -0.2 515,282 - GFF1196 0.70 -0.6 515,393 - GFF1196 0.81 +0.3 515,599 + +1.3 515,599 + -0.7 515,599 + +0.0 515,599 + +0.7 515,599 + -0.5 515,599 + -2.1 515,599 + +0.8 515,599 + -0.6 515,599 + +0.1 515,599 + +0.4 515,600 - +0.1 515,600 - +0.8 515,600 - -0.5 515,600 - -0.6 515,600 - -0.3 515,600 - -0.3 515,600 - -0.5 515,600 - -0.1 515,600 - -0.0 515,600 - -0.3 515,600 - -0.2 515,600 - -0.3 515,656 + +0.8 515,656 + +0.5 515,657 - +0.3 515,657 - -0.8 515,663 + +1.5 515,663 + -0.8 515,663 + -0.5 515,663 + -0.4 515,663 + -0.6 515,663 + +0.3 515,664 - -0.4 515,664 - +0.9 515,664 - -1.6 515,664 - +0.5 515,664 - -0.1 515,664 - -0.0 515,988 + GFF1197 0.35 -0.8 516,058 - GFF1197 0.42 -0.8 516,058 - GFF1197 0.42 +0.7 516,058 - GFF1197 0.42 -0.8 516,058 - GFF1197 0.42 +1.3 516,058 - GFF1197 0.42 +0.3 516,171 + GFF1197 0.54 +0.1 516,171 + GFF1197 0.54 -1.5 516,171 + GFF1197 0.54 -0.2 516,171 + GFF1197 0.54 -0.7 516,183 + GFF1197 0.55 +0.8 516,183 + GFF1197 0.55 -0.5 516,183 + GFF1197 0.55 -1.5 516,183 + GFF1197 0.55 -1.5 516,183 + GFF1197 0.55 +0.2 516,183 + GFF1197 0.55 +0.1 516,183 + GFF1197 0.55 +0.5 516,184 - GFF1197 0.56 -1.3 516,184 - GFF1197 0.56 -1.0 516,184 - GFF1197 0.56 +0.2 516,184 - GFF1197 0.56 -0.9 516,588 - +0.7 516,640 + -0.1 516,640 + +0.9 516,640 + -1.0 516,640 + -0.2 516,640 + +0.5 516,640 + +0.8 516,641 - -0.4 516,641 - -0.5 516,641 - -1.2 516,641 - +0.0 516,641 - +0.9 516,641 - +1.5 516,641 - +0.7 516,641 - -0.4 516,641 - -0.5 516,641 - +2.7 516,872 + GFF1198 0.38 +1.2 516,872 + GFF1198 0.38 +0.0 516,872 + GFF1198 0.38 -0.1 516,872 + GFF1198 0.38 -0.7 516,872 + GFF1198 0.38 -0.6 516,872 + GFF1198 0.38 +1.2 516,872 + GFF1198 0.38 +1.6 516,873 - GFF1198 0.38 +0.4 516,873 - GFF1198 0.38 +0.2 516,873 - GFF1198 0.38 -0.2 516,873 - GFF1198 0.38 -0.1 516,873 - GFF1198 0.38 -0.3 517,387 - +0.7 517,457 + -1.2 517,457 + -0.1 517,457 + -1.8 517,458 - -2.8 517,517 + -0.6 517,517 + +0.2 517,517 + +0.6 517,517 + +0.4 517,517 + +0.4 517,518 - -1.2 517,518 - -1.6 517,518 - -0.5 517,518 - -0.5 517,519 + -0.1 517,519 + +0.1 517,519 + +0.2 517,520 - -0.3 517,520 - +0.9 517,520 - -1.1 517,520 - -0.7 517,520 - -0.8 517,520 - -0.5 517,520 - +0.2 517,520 - -0.0 517,520 - -0.4 517,553 + GFF1199 0.10 -2.1 517,553 + GFF1199 0.10 -0.4 517,554 - GFF1199 0.10 +0.9 517,554 - GFF1199 0.10 +0.3
Or see this region's nucleotide sequence