Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdK and ybdJ are separated by 65 nucleotides ybdJ and ybdF are separated by 64 nucleotides ybdF and nfsB are separated by 93 nucleotides nfsB and ybdG are separated by 107 nucleotides
BNILDI_22045: ybdK - YbdK family carboxylate-amine ligase, at 4,431,549 to 4,432,667
ybdK
BNILDI_22050: ybdJ - Uncharacterized protein YbdJ, at 4,432,733 to 4,432,981
ybdJ
BNILDI_22055: ybdF - MmcQ/YjbR family DNA-binding protein, at 4,433,046 to 4,433,414
ybdF
BNILDI_22060: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 4,433,508 to 4,434,161
nfsB
BNILDI_22065: ybdG - mechanosensitive ion channel YbdG, at 4,434,269 to 4,435,516
ybdG
Position (kb) 4433
4434
4435 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4432.517 kb on + strand, within ybdK at 4432.666 kb on - strand at 4432.666 kb on - strand at 4432.669 kb on + strand at 4432.712 kb on + strand at 4432.746 kb on + strand at 4432.980 kb on - strand at 4432.998 kb on + strand at 4433.245 kb on + strand, within ybdF at 4433.245 kb on + strand, within ybdF at 4433.246 kb on - strand, within ybdF at 4433.425 kb on + strand at 4433.426 kb on - strand at 4433.427 kb on + strand at 4433.446 kb on - strand at 4433.574 kb on - strand, within nfsB at 4433.614 kb on - strand, within nfsB at 4433.676 kb on - strand, within nfsB at 4433.731 kb on + strand, within nfsB at 4433.894 kb on + strand, within nfsB at 4433.895 kb on - strand, within nfsB at 4433.937 kb on + strand, within nfsB at 4433.937 kb on + strand, within nfsB at 4433.938 kb on - strand, within nfsB at 4434.054 kb on + strand, within nfsB at 4434.055 kb on - strand, within nfsB at 4434.055 kb on - strand, within nfsB at 4434.159 kb on + strand at 4434.159 kb on + strand at 4434.159 kb on + strand at 4434.254 kb on + strand at 4434.254 kb on + strand at 4434.254 kb on + strand at 4434.380 kb on - strand at 4434.380 kb on - strand at 4434.382 kb on + strand at 4434.382 kb on + strand at 4434.458 kb on - strand, within ybdG at 4434.458 kb on - strand, within ybdG at 4434.491 kb on - strand, within ybdG at 4434.559 kb on + strand, within ybdG at 4434.596 kb on + strand, within ybdG at 4434.597 kb on - strand, within ybdG at 4434.631 kb on + strand, within ybdG at 4434.687 kb on + strand, within ybdG at 4434.701 kb on - strand, within ybdG at 4434.847 kb on + strand, within ybdG at 4434.982 kb on + strand, within ybdG at 4434.982 kb on + strand, within ybdG at 4435.055 kb on - strand, within ybdG at 4435.141 kb on - strand, within ybdG at 4435.148 kb on + strand, within ybdG
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 4,432,517 + ybdK BNILDI_22045 0.87 -0.8 4,432,666 - +1.1 4,432,666 - +0.7 4,432,669 + -0.5 4,432,712 + +0.0 4,432,746 + +0.4 4,432,980 - +0.5 4,432,998 + -0.4 4,433,245 + ybdF BNILDI_22055 0.54 -0.2 4,433,245 + ybdF BNILDI_22055 0.54 -0.6 4,433,246 - ybdF BNILDI_22055 0.54 +0.0 4,433,425 + +2.3 4,433,426 - -1.0 4,433,427 + +1.8 4,433,446 - -1.2 4,433,574 - nfsB BNILDI_22060 0.10 +0.8 4,433,614 - nfsB BNILDI_22060 0.16 -0.9 4,433,676 - nfsB BNILDI_22060 0.26 -0.5 4,433,731 + nfsB BNILDI_22060 0.34 +0.2 4,433,894 + nfsB BNILDI_22060 0.59 +0.8 4,433,895 - nfsB BNILDI_22060 0.59 -1.7 4,433,937 + nfsB BNILDI_22060 0.66 -0.8 4,433,937 + nfsB BNILDI_22060 0.66 -0.3 4,433,938 - nfsB BNILDI_22060 0.66 +0.7 4,434,054 + nfsB BNILDI_22060 0.83 +0.6 4,434,055 - nfsB BNILDI_22060 0.84 -2.4 4,434,055 - nfsB BNILDI_22060 0.84 +0.3 4,434,159 + -0.5 4,434,159 + -0.0 4,434,159 + +0.5 4,434,254 + -2.0 4,434,254 + +0.4 4,434,254 + +0.5 4,434,380 - -0.6 4,434,380 - +0.2 4,434,382 + +1.0 4,434,382 + +0.2 4,434,458 - ybdG BNILDI_22065 0.15 -1.8 4,434,458 - ybdG BNILDI_22065 0.15 +2.8 4,434,491 - ybdG BNILDI_22065 0.18 -0.1 4,434,559 + ybdG BNILDI_22065 0.23 -0.4 4,434,596 + ybdG BNILDI_22065 0.26 -0.4 4,434,597 - ybdG BNILDI_22065 0.26 -0.5 4,434,631 + ybdG BNILDI_22065 0.29 +0.8 4,434,687 + ybdG BNILDI_22065 0.33 +0.2 4,434,701 - ybdG BNILDI_22065 0.35 +0.3 4,434,847 + ybdG BNILDI_22065 0.46 -0.4 4,434,982 + ybdG BNILDI_22065 0.57 -0.1 4,434,982 + ybdG BNILDI_22065 0.57 +0.1 4,435,055 - ybdG BNILDI_22065 0.63 -1.4 4,435,141 - ybdG BNILDI_22065 0.70 -1.2 4,435,148 + ybdG BNILDI_22065 0.70 +0.7
Or see this region's nucleotide sequence