Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaK and wcaL overlap by 4 nucleotides wcaL and wcaM are separated by 10 nucleotides
BNILDI_14640: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 2,956,437 to 2,957,717
wcaK
BNILDI_14645: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 2,957,714 to 2,958,934
wcaL
BNILDI_14650: wcaM - colanic acid biosynthesis protein WcaM, at 2,958,945 to 2,960,339
wcaM
Position (kb)
2957
2958
2959 Strain fitness (log2 ratio)
-2
-1
0
1 at 2956.736 kb on + strand, within wcaK at 2956.737 kb on - strand, within wcaK at 2956.833 kb on + strand, within wcaK at 2956.871 kb on + strand, within wcaK at 2956.934 kb on + strand, within wcaK at 2956.934 kb on + strand, within wcaK at 2956.943 kb on - strand, within wcaK at 2956.950 kb on + strand, within wcaK at 2957.021 kb on + strand, within wcaK at 2957.101 kb on - strand, within wcaK at 2957.298 kb on + strand, within wcaK at 2957.298 kb on + strand, within wcaK at 2957.299 kb on - strand, within wcaK at 2957.299 kb on - strand, within wcaK at 2957.299 kb on - strand, within wcaK at 2957.300 kb on + strand, within wcaK at 2957.368 kb on + strand, within wcaK at 2957.369 kb on - strand, within wcaK at 2957.369 kb on - strand, within wcaK at 2957.456 kb on + strand, within wcaK at 2957.457 kb on - strand, within wcaK at 2957.504 kb on + strand, within wcaK at 2957.505 kb on - strand, within wcaK at 2957.556 kb on - strand, within wcaK at 2957.567 kb on - strand, within wcaK at 2957.868 kb on - strand, within wcaL at 2957.908 kb on + strand, within wcaL at 2957.909 kb on - strand, within wcaL at 2957.909 kb on - strand, within wcaL at 2957.974 kb on + strand, within wcaL at 2958.158 kb on - strand, within wcaL at 2958.331 kb on - strand, within wcaL at 2958.331 kb on - strand, within wcaL at 2958.334 kb on + strand, within wcaL at 2958.536 kb on + strand, within wcaL at 2958.537 kb on - strand, within wcaL at 2958.571 kb on + strand, within wcaL at 2958.884 kb on - strand at 2958.932 kb on + strand at 2958.933 kb on - strand at 2958.953 kb on + strand at 2959.062 kb on + strand at 2959.238 kb on - strand, within wcaM at 2959.277 kb on + strand, within wcaM at 2959.277 kb on + strand, within wcaM at 2959.278 kb on - strand, within wcaM at 2959.284 kb on - strand, within wcaM at 2959.365 kb on + strand, within wcaM at 2959.366 kb on - strand, within wcaM at 2959.367 kb on + strand, within wcaM at 2959.367 kb on + strand, within wcaM at 2959.368 kb on - strand, within wcaM at 2959.438 kb on - strand, within wcaM at 2959.595 kb on + strand, within wcaM at 2959.647 kb on + strand, within wcaM at 2959.648 kb on - strand, within wcaM at 2959.923 kb on + strand, within wcaM
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 2,956,736 + wcaK BNILDI_14640 0.23 +0.9 2,956,737 - wcaK BNILDI_14640 0.23 -0.0 2,956,833 + wcaK BNILDI_14640 0.31 -0.7 2,956,871 + wcaK BNILDI_14640 0.34 -0.2 2,956,934 + wcaK BNILDI_14640 0.39 -0.4 2,956,934 + wcaK BNILDI_14640 0.39 +1.0 2,956,943 - wcaK BNILDI_14640 0.40 -0.7 2,956,950 + wcaK BNILDI_14640 0.40 -1.2 2,957,021 + wcaK BNILDI_14640 0.46 +0.8 2,957,101 - wcaK BNILDI_14640 0.52 -0.2 2,957,298 + wcaK BNILDI_14640 0.67 -0.3 2,957,298 + wcaK BNILDI_14640 0.67 +0.3 2,957,299 - wcaK BNILDI_14640 0.67 +0.4 2,957,299 - wcaK BNILDI_14640 0.67 +0.0 2,957,299 - wcaK BNILDI_14640 0.67 -0.7 2,957,300 + wcaK BNILDI_14640 0.67 -0.4 2,957,368 + wcaK BNILDI_14640 0.73 +0.6 2,957,369 - wcaK BNILDI_14640 0.73 -0.3 2,957,369 - wcaK BNILDI_14640 0.73 -0.8 2,957,456 + wcaK BNILDI_14640 0.80 -0.2 2,957,457 - wcaK BNILDI_14640 0.80 -0.7 2,957,504 + wcaK BNILDI_14640 0.83 -1.1 2,957,505 - wcaK BNILDI_14640 0.83 -0.5 2,957,556 - wcaK BNILDI_14640 0.87 -0.3 2,957,567 - wcaK BNILDI_14640 0.88 +1.1 2,957,868 - wcaL BNILDI_14645 0.13 +0.1 2,957,908 + wcaL BNILDI_14645 0.16 -0.8 2,957,909 - wcaL BNILDI_14645 0.16 +0.0 2,957,909 - wcaL BNILDI_14645 0.16 -0.3 2,957,974 + wcaL BNILDI_14645 0.21 -0.4 2,958,158 - wcaL BNILDI_14645 0.36 +0.4 2,958,331 - wcaL BNILDI_14645 0.51 -0.4 2,958,331 - wcaL BNILDI_14645 0.51 +0.6 2,958,334 + wcaL BNILDI_14645 0.51 -1.9 2,958,536 + wcaL BNILDI_14645 0.67 -0.2 2,958,537 - wcaL BNILDI_14645 0.67 -1.4 2,958,571 + wcaL BNILDI_14645 0.70 -1.3 2,958,884 - +0.9 2,958,932 + +1.6 2,958,933 - -0.4 2,958,953 + +1.6 2,959,062 + +0.0 2,959,238 - wcaM BNILDI_14650 0.21 +0.2 2,959,277 + wcaM BNILDI_14650 0.24 +0.4 2,959,277 + wcaM BNILDI_14650 0.24 -1.1 2,959,278 - wcaM BNILDI_14650 0.24 +0.4 2,959,284 - wcaM BNILDI_14650 0.24 -0.3 2,959,365 + wcaM BNILDI_14650 0.30 -0.3 2,959,366 - wcaM BNILDI_14650 0.30 +0.1 2,959,367 + wcaM BNILDI_14650 0.30 -0.5 2,959,367 + wcaM BNILDI_14650 0.30 +0.4 2,959,368 - wcaM BNILDI_14650 0.30 +0.6 2,959,438 - wcaM BNILDI_14650 0.35 -0.6 2,959,595 + wcaM BNILDI_14650 0.47 +0.4 2,959,647 + wcaM BNILDI_14650 0.50 -0.8 2,959,648 - wcaM BNILDI_14650 0.50 -0.5 2,959,923 + wcaM BNILDI_14650 0.70 -1.8
Or see this region's nucleotide sequence