Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxC and secM are separated by 155 nucleotides secM and secA are separated by 61 nucleotides secA and mutT are separated by 59 nucleotides mutT and yacG are separated by 99 nucleotides yacG and zapD are separated by 9 nucleotides
BNILDI_08665: lpxC - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 1,735,765 to 1,736,682
lpxC
BNILDI_08670: secM - secA translation cis-regulator SecM, at 1,736,838 to 1,737,425
secM
BNILDI_08675: secA - preprotein translocase subunit SecA, at 1,737,487 to 1,740,192
secA
BNILDI_08685: mutT - 8-oxo-dGTP diphosphatase MutT, at 1,740,252 to 1,740,641
mutT
BNILDI_08695: yacG - DNA gyrase inhibitor YacG, at 1,740,741 to 1,740,938
yacG
BNILDI_08700: zapD - cell division protein ZapD, at 1,740,948 to 1,741,691
zapD
Position (kb)
1737
1738
1739
1740
1741 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.690 kb on + strand at 1736.691 kb on - strand at 1736.692 kb on + strand at 1736.700 kb on + strand at 1736.769 kb on - strand at 1736.777 kb on + strand at 1736.827 kb on + strand at 1736.827 kb on + strand at 1736.858 kb on + strand at 1736.974 kb on + strand, within secM at 1737.423 kb on + strand at 1737.432 kb on + strand at 1737.453 kb on + strand at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.982 kb on + strand at 1739.983 kb on - strand at 1739.983 kb on - strand at 1740.057 kb on + strand at 1740.058 kb on - strand at 1740.190 kb on + strand at 1740.190 kb on + strand at 1740.278 kb on + strand at 1740.469 kb on - strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.489 kb on - strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.491 kb on - strand, within mutT at 1740.572 kb on - strand, within mutT at 1740.581 kb on + strand, within mutT at 1740.581 kb on + strand, within mutT at 1740.629 kb on + strand at 1740.719 kb on + strand at 1740.720 kb on - strand at 1740.736 kb on - strand at 1740.926 kb on + strand at 1740.927 kb on - strand at 1741.037 kb on + strand, within zapD at 1741.037 kb on + strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.170 kb on + strand, within zapD at 1741.187 kb on + strand, within zapD at 1741.187 kb on + strand, within zapD at 1741.188 kb on - strand, within zapD
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 1,736,690 + -0.9 1,736,690 + -1.6 1,736,690 + +2.6 1,736,690 + -1.6 1,736,690 + -0.5 1,736,690 + -0.8 1,736,690 + +1.2 1,736,691 - +0.3 1,736,692 + +0.0 1,736,700 + +0.9 1,736,769 - -0.2 1,736,777 + -0.2 1,736,827 + -1.0 1,736,827 + -0.2 1,736,858 + -0.1 1,736,974 + secM BNILDI_08670 0.23 -0.3 1,737,423 + -0.7 1,737,432 + -0.2 1,737,453 + +0.3 1,739,978 + +0.1 1,739,978 + -0.5 1,739,978 + -2.1 1,739,978 + +0.2 1,739,982 + -0.5 1,739,983 - -0.5 1,739,983 - -0.8 1,740,057 + +0.4 1,740,058 - +0.8 1,740,190 + -0.0 1,740,190 + +0.2 1,740,278 + -0.8 1,740,469 - mutT BNILDI_08685 0.56 -0.4 1,740,488 + mutT BNILDI_08685 0.61 +0.1 1,740,488 + mutT BNILDI_08685 0.61 +0.0 1,740,488 + mutT BNILDI_08685 0.61 +0.2 1,740,489 - mutT BNILDI_08685 0.61 +0.3 1,740,490 + mutT BNILDI_08685 0.61 -1.4 1,740,490 + mutT BNILDI_08685 0.61 +0.5 1,740,490 + mutT BNILDI_08685 0.61 +0.7 1,740,490 + mutT BNILDI_08685 0.61 -0.0 1,740,490 + mutT BNILDI_08685 0.61 +1.0 1,740,490 + mutT BNILDI_08685 0.61 -0.4 1,740,490 + mutT BNILDI_08685 0.61 +0.1 1,740,491 - mutT BNILDI_08685 0.61 -0.1 1,740,572 - mutT BNILDI_08685 0.82 +0.2 1,740,581 + mutT BNILDI_08685 0.84 +0.1 1,740,581 + mutT BNILDI_08685 0.84 -0.8 1,740,629 + -0.3 1,740,719 + -0.6 1,740,720 - +0.4 1,740,736 - +0.2 1,740,926 + -1.2 1,740,927 - +0.5 1,741,037 + zapD BNILDI_08700 0.12 -0.2 1,741,037 + zapD BNILDI_08700 0.12 -0.6 1,741,038 - zapD BNILDI_08700 0.12 -0.6 1,741,038 - zapD BNILDI_08700 0.12 -0.4 1,741,038 - zapD BNILDI_08700 0.12 +0.3 1,741,038 - zapD BNILDI_08700 0.12 +0.5 1,741,038 - zapD BNILDI_08700 0.12 -0.4 1,741,038 - zapD BNILDI_08700 0.12 +0.6 1,741,170 + zapD BNILDI_08700 0.30 +0.2 1,741,187 + zapD BNILDI_08700 0.32 +0.1 1,741,187 + zapD BNILDI_08700 0.32 +0.0 1,741,188 - zapD BNILDI_08700 0.32 +0.1
Or see this region's nucleotide sequence