Experiment: JK36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hpaH and hpcD are separated by 110 nucleotides hpcD and hpaD are separated by 9 nucleotides hpaD and hpaE are separated by 1 nucleotides
BNILDI_07865: hpaH - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 1,573,082 to 1,573,885
hpaH
BNILDI_07870: hpcD - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 1,573,996 to 1,574,376
hpcD
BNILDI_07875: hpaD - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,574,386 to 1,575,237
hpaD
BNILDI_07880: hpaE - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 1,575,239 to 1,576,705
hpaE
Position (kb) 1574
1575
1576 Strain fitness (log2 ratio) -1
0
1
2 at 1573.503 kb on + strand, within hpaH at 1573.503 kb on + strand, within hpaH at 1573.503 kb on + strand, within hpaH at 1573.503 kb on + strand, within hpaH at 1573.504 kb on - strand, within hpaH at 1573.870 kb on + strand at 1573.940 kb on + strand at 1573.941 kb on - strand at 1574.188 kb on - strand, within hpcD at 1574.364 kb on + strand at 1574.440 kb on + strand at 1574.472 kb on - strand, within hpaD at 1574.517 kb on + strand, within hpaD at 1574.518 kb on - strand, within hpaD at 1574.518 kb on - strand, within hpaD at 1574.538 kb on - strand, within hpaD at 1574.559 kb on + strand, within hpaD at 1574.560 kb on - strand, within hpaD at 1574.560 kb on - strand, within hpaD at 1574.639 kb on + strand, within hpaD at 1574.639 kb on + strand, within hpaD at 1574.640 kb on - strand, within hpaD at 1574.640 kb on - strand, within hpaD at 1574.934 kb on + strand, within hpaD at 1574.964 kb on - strand, within hpaD at 1574.964 kb on - strand, within hpaD at 1574.969 kb on + strand, within hpaD at 1574.969 kb on + strand, within hpaD at 1574.969 kb on + strand, within hpaD at 1574.969 kb on + strand, within hpaD at 1574.970 kb on - strand, within hpaD at 1574.985 kb on + strand, within hpaD at 1575.011 kb on + strand, within hpaD at 1575.047 kb on + strand, within hpaD at 1575.188 kb on + strand at 1575.199 kb on + strand at 1575.231 kb on + strand at 1575.232 kb on - strand at 1575.232 kb on - strand at 1575.319 kb on - strand at 1575.319 kb on - strand at 1575.354 kb on - strand at 1575.354 kb on - strand at 1575.469 kb on - strand, within hpaE at 1575.507 kb on - strand, within hpaE at 1575.984 kb on + strand, within hpaE at 1575.985 kb on - strand, within hpaE at 1576.055 kb on + strand, within hpaE at 1576.056 kb on - strand, within hpaE at 1576.056 kb on - strand, within hpaE at 1576.056 kb on - strand, within hpaE at 1576.107 kb on - strand, within hpaE
Per-strain Table
Position Strand Gene LocusTag Fraction JK36 remove 1,573,503 + hpaH BNILDI_07865 0.52 +0.1 1,573,503 + hpaH BNILDI_07865 0.52 +0.5 1,573,503 + hpaH BNILDI_07865 0.52 +0.1 1,573,503 + hpaH BNILDI_07865 0.52 +0.1 1,573,504 - hpaH BNILDI_07865 0.52 +1.1 1,573,870 + -0.9 1,573,940 + -0.3 1,573,941 - -0.6 1,574,188 - hpcD BNILDI_07870 0.50 -0.7 1,574,364 + +0.6 1,574,440 + -0.9 1,574,472 - hpaD BNILDI_07875 0.10 +0.1 1,574,517 + hpaD BNILDI_07875 0.15 +1.9 1,574,518 - hpaD BNILDI_07875 0.15 -0.5 1,574,518 - hpaD BNILDI_07875 0.15 -0.2 1,574,538 - hpaD BNILDI_07875 0.18 -0.7 1,574,559 + hpaD BNILDI_07875 0.20 +1.0 1,574,560 - hpaD BNILDI_07875 0.20 -1.4 1,574,560 - hpaD BNILDI_07875 0.20 -0.2 1,574,639 + hpaD BNILDI_07875 0.30 +1.1 1,574,639 + hpaD BNILDI_07875 0.30 -0.3 1,574,640 - hpaD BNILDI_07875 0.30 +0.4 1,574,640 - hpaD BNILDI_07875 0.30 +0.4 1,574,934 + hpaD BNILDI_07875 0.64 -0.5 1,574,964 - hpaD BNILDI_07875 0.68 -0.7 1,574,964 - hpaD BNILDI_07875 0.68 -0.3 1,574,969 + hpaD BNILDI_07875 0.68 +0.4 1,574,969 + hpaD BNILDI_07875 0.68 -1.3 1,574,969 + hpaD BNILDI_07875 0.68 -0.4 1,574,969 + hpaD BNILDI_07875 0.68 +0.4 1,574,970 - hpaD BNILDI_07875 0.69 -1.0 1,574,985 + hpaD BNILDI_07875 0.70 +1.0 1,575,011 + hpaD BNILDI_07875 0.73 -1.0 1,575,047 + hpaD BNILDI_07875 0.78 +0.3 1,575,188 + +0.4 1,575,199 + -0.2 1,575,231 + -1.1 1,575,232 - -0.7 1,575,232 - +0.0 1,575,319 - -0.1 1,575,319 - +0.5 1,575,354 - -0.1 1,575,354 - -1.0 1,575,469 - hpaE BNILDI_07880 0.16 -0.5 1,575,507 - hpaE BNILDI_07880 0.18 -0.1 1,575,984 + hpaE BNILDI_07880 0.51 -0.1 1,575,985 - hpaE BNILDI_07880 0.51 -1.5 1,576,055 + hpaE BNILDI_07880 0.56 +0.4 1,576,056 - hpaE BNILDI_07880 0.56 -0.1 1,576,056 - hpaE BNILDI_07880 0.56 +0.9 1,576,056 - hpaE BNILDI_07880 0.56 -0.3 1,576,107 - hpaE BNILDI_07880 0.59 +2.3
Or see this region's nucleotide sequence