Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF870 and GFF871 are separated by 20 nucleotides GFF871 and GFF872 overlap by 4 nucleotides GFF872 and GFF873 overlap by 4 nucleotides GFF873 and GFF874 overlap by 4 nucleotides
GFF870 - TonB-dependent receptor, at 178,388 to 180,517
GFF870
GFF871 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 180,538 to 181,431
GFF871
GFF872 - Hemin ABC transporter, permease protein, at 181,428 to 182,420
GFF872
GFF873 - KEGG: iron complex transport system ATP-binding protein , at 182,417 to 183,151
GFF873
GFF874 - no description, at 183,148 to 184,188
GFF874
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2 at 180.428 kb on + strand at 180.428 kb on + strand at 180.429 kb on - strand at 180.429 kb on - strand at 180.429 kb on - strand at 180.429 kb on - strand at 180.431 kb on + strand at 180.431 kb on + strand at 180.431 kb on + strand at 180.432 kb on - strand at 180.506 kb on + strand at 180.507 kb on - strand at 180.507 kb on - strand at 180.802 kb on + strand, within GFF871 at 180.863 kb on - strand, within GFF871 at 181.075 kb on + strand, within GFF871 at 181.075 kb on + strand, within GFF871 at 181.076 kb on - strand, within GFF871 at 181.132 kb on + strand, within GFF871 at 181.132 kb on + strand, within GFF871 at 181.396 kb on + strand at 181.396 kb on + strand at 181.688 kb on + strand, within GFF872 at 181.689 kb on + strand, within GFF872 at 181.690 kb on - strand, within GFF872 at 181.929 kb on + strand, within GFF872 at 182.446 kb on - strand at 182.446 kb on - strand at 182.446 kb on - strand at 182.664 kb on - strand, within GFF873 at 183.084 kb on - strand at 183.084 kb on - strand at 183.317 kb on - strand, within GFF874
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 180,428 + -0.8 180,428 + +0.2 180,429 - -0.4 180,429 - -0.6 180,429 - +1.0 180,429 - -1.7 180,431 + -1.3 180,431 + -0.4 180,431 + -0.1 180,432 - -0.5 180,506 + +0.0 180,507 - -0.2 180,507 - -0.1 180,802 + GFF871 0.30 -0.0 180,863 - GFF871 0.36 -1.7 181,075 + GFF871 0.60 +0.7 181,075 + GFF871 0.60 -0.1 181,076 - GFF871 0.60 +2.1 181,132 + GFF871 0.66 +0.6 181,132 + GFF871 0.66 +1.5 181,396 + -1.0 181,396 + +0.2 181,688 + GFF872 0.26 +0.3 181,689 + GFF872 0.26 +0.0 181,690 - GFF872 0.26 +1.6 181,929 + GFF872 0.50 -0.7 182,446 - +0.1 182,446 - +2.2 182,446 - -0.2 182,664 - GFF873 0.34 +1.1 183,084 - -0.4 183,084 - -0.1 183,317 - GFF874 0.16 +0.3
Or see this region's nucleotide sequence