Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF867 and GFF868 are separated by 8 nucleotides GFF868 and GFF869 are separated by 153 nucleotides
GFF867 - Oxidoreductase, GMC family, at 174,599 to 176,224
GFF867
GFF868 - Uracil-DNA glycosylase, family 1, at 176,233 to 177,084
GFF868
GFF869 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 177,238 to 177,891
GFF869
Position (kb) 176
177
178 Strain fitness (log2 ratio) -2
-1
0
1
2 at 175.290 kb on + strand, within GFF867 at 175.291 kb on - strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.318 kb on - strand, within GFF867 at 175.869 kb on + strand, within GFF867 at 175.869 kb on + strand, within GFF867 at 175.870 kb on - strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.933 kb on - strand, within GFF867 at 175.986 kb on + strand, within GFF867 at 175.986 kb on + strand, within GFF867 at 176.199 kb on + strand at 176.199 kb on + strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.808 kb on + strand, within GFF868 at 176.809 kb on - strand, within GFF868 at 177.136 kb on - strand at 177.139 kb on - strand at 177.418 kb on + strand, within GFF869 at 177.419 kb on - strand, within GFF869 at 177.421 kb on + strand, within GFF869 at 177.421 kb on + strand, within GFF869 at 177.421 kb on + strand, within GFF869 at 177.422 kb on - strand, within GFF869 at 177.422 kb on - strand, within GFF869 at 177.422 kb on - strand, within GFF869 at 177.424 kb on + strand, within GFF869 at 177.424 kb on + strand, within GFF869 at 177.424 kb on + strand, within GFF869 at 177.424 kb on + strand, within GFF869 at 177.425 kb on - strand, within GFF869 at 177.425 kb on - strand, within GFF869 at 177.449 kb on - strand, within GFF869 at 177.449 kb on - strand, within GFF869 at 177.449 kb on - strand, within GFF869 at 177.542 kb on - strand, within GFF869 at 177.767 kb on + strand, within GFF869 at 177.770 kb on - strand, within GFF869 at 177.770 kb on - strand, within GFF869 at 177.778 kb on + strand, within GFF869 at 177.922 kb on + strand at 177.925 kb on - strand at 177.955 kb on + strand at 178.010 kb on + strand at 178.010 kb on + strand at 178.011 kb on - strand at 178.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 175,290 + GFF867 0.42 +0.6 175,291 - GFF867 0.43 +0.4 175,317 + GFF867 0.44 +0.1 175,317 + GFF867 0.44 +0.2 175,317 + GFF867 0.44 +0.9 175,318 - GFF867 0.44 -0.0 175,869 + GFF867 0.78 +0.0 175,869 + GFF867 0.78 +0.3 175,870 - GFF867 0.78 -0.1 175,932 + GFF867 0.82 +0.9 175,932 + GFF867 0.82 +0.1 175,932 + GFF867 0.82 +1.1 175,932 + GFF867 0.82 -0.9 175,933 - GFF867 0.82 -0.1 175,986 + GFF867 0.85 -0.8 175,986 + GFF867 0.85 +0.9 176,199 + +0.5 176,199 + -0.0 176,200 - +0.2 176,200 - -0.4 176,200 - -1.8 176,200 - -1.5 176,808 + GFF868 0.67 +2.2 176,809 - GFF868 0.68 +0.6 177,136 - +0.0 177,139 - -0.5 177,418 + GFF869 0.28 -1.4 177,419 - GFF869 0.28 -1.5 177,421 + GFF869 0.28 +1.0 177,421 + GFF869 0.28 -0.4 177,421 + GFF869 0.28 -0.3 177,422 - GFF869 0.28 +0.7 177,422 - GFF869 0.28 +0.5 177,422 - GFF869 0.28 -0.6 177,424 + GFF869 0.28 +0.6 177,424 + GFF869 0.28 -0.4 177,424 + GFF869 0.28 -1.0 177,424 + GFF869 0.28 -0.3 177,425 - GFF869 0.29 -1.3 177,425 - GFF869 0.29 -0.1 177,449 - GFF869 0.32 -0.1 177,449 - GFF869 0.32 -0.6 177,449 - GFF869 0.32 -0.8 177,542 - GFF869 0.46 +0.2 177,767 + GFF869 0.81 +0.9 177,770 - GFF869 0.81 -0.1 177,770 - GFF869 0.81 -0.4 177,778 + GFF869 0.83 +0.7 177,922 + -0.8 177,925 - +0.7 177,955 + -0.1 178,010 + -0.5 178,010 + +0.1 178,011 - -0.7 178,026 - +0.5
Or see this region's nucleotide sequence