Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF747 and GFF748 are separated by 15 nucleotides GFF748 and GFF749 are separated by 2 nucleotides GFF749 and GFF750 are separated by 168 nucleotides
GFF747 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 10,429 to 11,733
GFF747
GFF748 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase (EC 4.1.3.17), at 11,749 to 12,447
GFF748
GFF749 - 4-oxalomesaconate hydratase (EC 4.2.1.83), at 12,450 to 13,478
GFF749
GFF750 - Transcriptional regulator LigR, LysR family, at 13,647 to 14,924
GFF750
Position (kb)
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 11.531 kb on + strand, within GFF747 at 11.531 kb on + strand, within GFF747 at 11.532 kb on - strand, within GFF747 at 11.532 kb on - strand, within GFF747 at 11.567 kb on + strand, within GFF747 at 11.567 kb on + strand, within GFF747 at 11.568 kb on - strand, within GFF747 at 11.568 kb on - strand, within GFF747 at 11.759 kb on + strand at 11.760 kb on - strand at 11.760 kb on - strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.798 kb on + strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 11.799 kb on - strand at 12.515 kb on - strand at 12.515 kb on - strand at 12.515 kb on - strand at 12.562 kb on + strand, within GFF749 at 12.563 kb on - strand, within GFF749 at 12.563 kb on - strand, within GFF749 at 12.580 kb on + strand, within GFF749 at 12.694 kb on + strand, within GFF749 at 12.695 kb on - strand, within GFF749 at 12.695 kb on - strand, within GFF749 at 12.695 kb on - strand, within GFF749 at 12.790 kb on + strand, within GFF749 at 13.453 kb on + strand at 13.454 kb on - strand at 13.560 kb on + strand at 13.560 kb on + strand at 13.560 kb on + strand at 13.560 kb on + strand at 13.561 kb on - strand at 14.208 kb on + strand, within GFF750 at 14.209 kb on - strand, within GFF750 at 14.209 kb on - strand, within GFF750 at 14.394 kb on + strand, within GFF750 at 14.395 kb on - strand, within GFF750 at 14.395 kb on - strand, within GFF750
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 11,531 + GFF747 0.84 -0.1 11,531 + GFF747 0.84 -2.3 11,532 - GFF747 0.85 -0.6 11,532 - GFF747 0.85 -1.5 11,567 + GFF747 0.87 -0.9 11,567 + GFF747 0.87 -0.1 11,568 - GFF747 0.87 -0.9 11,568 - GFF747 0.87 -0.4 11,759 + +0.0 11,760 - +0.3 11,760 - -1.8 11,798 + -1.9 11,798 + +0.4 11,798 + -1.1 11,798 + -0.1 11,798 + +1.3 11,798 + -0.5 11,798 + +1.7 11,798 + -1.1 11,799 - +1.1 11,799 - -0.6 11,799 - +0.0 11,799 - +0.1 11,799 - -1.1 11,799 - -1.1 11,799 - +0.5 11,799 - -2.3 12,515 - -0.8 12,515 - -0.8 12,515 - -0.8 12,562 + GFF749 0.11 -0.2 12,563 - GFF749 0.11 +1.2 12,563 - GFF749 0.11 -0.3 12,580 + GFF749 0.13 -2.8 12,694 + GFF749 0.24 -0.4 12,695 - GFF749 0.24 -1.5 12,695 - GFF749 0.24 -0.1 12,695 - GFF749 0.24 -0.4 12,790 + GFF749 0.33 -0.9 13,453 + -1.4 13,454 - -0.8 13,560 + +0.0 13,560 + -0.3 13,560 + -0.7 13,560 + -0.2 13,561 - +0.3 14,208 + GFF750 0.44 -0.3 14,209 - GFF750 0.44 -0.4 14,209 - GFF750 0.44 -0.8 14,394 + GFF750 0.58 -0.6 14,395 - GFF750 0.59 -0.1 14,395 - GFF750 0.59 -1.1
Or see this region's nucleotide sequence