Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7336 and GFF7337 are separated by 194 nucleotides GFF7337 and GFF7338 overlap by 8 nucleotides GFF7338 and GFF7339 overlap by 4 nucleotides
GFF7336 - Alkanesulfonate utilization operon LysR-family regulator Cbl, at 5,045 to 5,980
GFF7336
GFF7337 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 6,175 to 7,137
GFF7337
GFF7338 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,130 to 7,957
GFF7338
GFF7339 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 7,954 to 8,781
GFF7339
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5.281 kb on - strand, within GFF7336 at 5.389 kb on - strand, within GFF7336 at 5.721 kb on + strand, within GFF7336 at 5.722 kb on - strand, within GFF7336 at 6.065 kb on - strand at 6.300 kb on - strand, within GFF7337 at 6.381 kb on - strand, within GFF7337 at 6.537 kb on - strand, within GFF7337 at 6.638 kb on + strand, within GFF7337 at 6.639 kb on - strand, within GFF7337 at 7.239 kb on + strand, within GFF7338 at 7.342 kb on - strand, within GFF7338 at 7.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 5,281 - GFF7336 0.25 +1.7 5,389 - GFF7336 0.37 -3.0 5,721 + GFF7336 0.72 -0.5 5,722 - GFF7336 0.72 +0.6 6,065 - -0.1 6,300 - GFF7337 0.13 -1.7 6,381 - GFF7337 0.21 -2.2 6,537 - GFF7337 0.38 -0.5 6,638 + GFF7337 0.48 -2.3 6,639 - GFF7337 0.48 +0.7 7,239 + GFF7338 0.13 +1.7 7,342 - GFF7338 0.26 -0.7 7,947 - +0.0
Or see this region's nucleotide sequence