Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7201 and GFF7202 are separated by 17 nucleotides GFF7202 and GFF7203 are separated by 7 nucleotides GFF7203 and GFF7204 are separated by 176 nucleotides GFF7204 and GFF7205 are separated by 28 nucleotides
GFF7201 - Alkanesulfonates transport system permease protein, at 19,437 to 20,252
GFF7201
GFF7202 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 20,270 to 21,142
GFF7202
GFF7203 - Two-component transcriptional response regulator, LuxR family, at 21,150 to 22,622
GFF7203
GFF7204 - no description, at 22,799 to 23,386
GFF7204
GFF7205 - no description, at 23,415 to 23,612
GFF7205
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.333 kb on + strand at 20.333 kb on + strand at 20.333 kb on + strand at 20.333 kb on + strand at 20.334 kb on - strand at 20.334 kb on - strand at 20.484 kb on - strand, within GFF7202 at 20.622 kb on - strand, within GFF7202 at 20.622 kb on - strand, within GFF7202 at 21.152 kb on - strand at 21.387 kb on + strand, within GFF7203 at 21.387 kb on + strand, within GFF7203 at 21.387 kb on + strand, within GFF7203 at 21.388 kb on - strand, within GFF7203 at 21.676 kb on + strand, within GFF7203 at 21.676 kb on + strand, within GFF7203 at 21.787 kb on + strand, within GFF7203 at 21.787 kb on + strand, within GFF7203 at 21.788 kb on - strand, within GFF7203 at 21.788 kb on - strand, within GFF7203 at 22.043 kb on - strand, within GFF7203 at 22.093 kb on + strand, within GFF7203 at 22.195 kb on + strand, within GFF7203 at 22.195 kb on + strand, within GFF7203 at 22.196 kb on - strand, within GFF7203 at 22.853 kb on + strand at 22.854 kb on - strand at 22.854 kb on - strand at 22.856 kb on + strand at 22.857 kb on - strand at 22.857 kb on - strand at 23.309 kb on + strand, within GFF7204 at 23.309 kb on + strand, within GFF7204 at 23.309 kb on + strand, within GFF7204 at 23.309 kb on + strand, within GFF7204 at 23.310 kb on - strand, within GFF7204 at 23.425 kb on + strand at 23.426 kb on - strand at 23.426 kb on - strand at 23.426 kb on - strand at 23.426 kb on - strand at 23.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 20,333 + +0.2 20,333 + -0.2 20,333 + +0.0 20,333 + -0.1 20,334 - -0.6 20,334 - -0.6 20,484 - GFF7202 0.25 -0.8 20,622 - GFF7202 0.40 -0.1 20,622 - GFF7202 0.40 +0.4 21,152 - -1.2 21,387 + GFF7203 0.16 -1.1 21,387 + GFF7203 0.16 +0.2 21,387 + GFF7203 0.16 +1.6 21,388 - GFF7203 0.16 -0.7 21,676 + GFF7203 0.36 +0.6 21,676 + GFF7203 0.36 -0.3 21,787 + GFF7203 0.43 +0.0 21,787 + GFF7203 0.43 -0.8 21,788 - GFF7203 0.43 +0.2 21,788 - GFF7203 0.43 -0.8 22,043 - GFF7203 0.61 +0.6 22,093 + GFF7203 0.64 +1.5 22,195 + GFF7203 0.71 +2.6 22,195 + GFF7203 0.71 +0.3 22,196 - GFF7203 0.71 +0.1 22,853 + +0.7 22,854 - -1.9 22,854 - -0.1 22,856 + -0.3 22,857 - -0.5 22,857 - -0.5 23,309 + GFF7204 0.87 -0.5 23,309 + GFF7204 0.87 +1.1 23,309 + GFF7204 0.87 -0.3 23,309 + GFF7204 0.87 +0.7 23,310 - GFF7204 0.87 +0.5 23,425 + -0.1 23,426 - +0.4 23,426 - -0.1 23,426 - -0.5 23,426 - +0.2 23,426 - -0.2
Or see this region's nucleotide sequence