Strain Fitness in Variovorax sp. SCN45 around GFF6997

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6995 and GFF6996 overlap by 4 nucleotidesGFF6996 and GFF6997 overlap by 4 nucleotidesGFF6997 and GFF6998 are separated by 1 nucleotidesGFF6998 and GFF6999 are separated by 46 nucleotides GFF6995 - Flagellar M-ring protein FliF, at 12,387 to 13,901 GFF6995 GFF6996 - Flagellar hook-basal body complex protein FliE, at 13,898 to 14,263 GFF6996 GFF6997 - Flagellar basal-body rod protein FlgC, at 14,260 to 14,709 GFF6997 GFF6998 - Flagellar basal-body rod protein FlgB, at 14,711 to 15,094 GFF6998 GFF6999 - Flagellar P-ring protein FlgI, at 15,141 to 16,277 GFF6999 Position (kb) 14 15Strain fitness (log2 ratio) -1 0 1at 13.880 kb on + strandat 13.880 kb on + strandat 13.901 kb on + strandat 13.915 kb on + strandat 14.048 kb on + strand, within GFF6996at 14.337 kb on - strand, within GFF6997at 14.439 kb on - strand, within GFF6997at 14.702 kb on + strandat 14.915 kb on + strand, within GFF6998at 14.979 kb on + strand, within GFF6998

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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13,880 + +0.2
13,880 + +0.3
13,901 + +0.4
13,915 + +0.1
14,048 + GFF6996 0.41 -0.6
14,337 - GFF6997 0.17 -0.5
14,439 - GFF6997 0.40 -1.6
14,702 + -1.0
14,915 + GFF6998 0.53 -0.4
14,979 + GFF6998 0.70 -0.5

Or see this region's nucleotide sequence