Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6750 and GFF6751 are separated by 29 nucleotides GFF6751 and GFF6752 overlap by 4 nucleotides
GFF6750 - hypothetical protein, at 63,530 to 64,090
GFF6750
GFF6751 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 64,120 to 64,887
GFF6751
GFF6752 - 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (EC 1.2.1.91) / 2-oxepin-2(3H)-ylideneacetyl-CoA hydrolase (EC 3.3.2.12), at 64,884 to 66,944
GFF6752
Position (kb) 64
65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 63.990 kb on + strand, within GFF6750 at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.035 kb on + strand at 64.036 kb on - strand at 64.036 kb on - strand at 64.036 kb on - strand at 64.036 kb on - strand at 64.036 kb on - strand at 64.036 kb on - strand at 64.103 kb on + strand at 64.104 kb on - strand at 64.121 kb on + strand at 64.121 kb on + strand at 64.121 kb on + strand at 64.173 kb on - strand at 64.173 kb on - strand at 64.173 kb on - strand at 64.173 kb on - strand at 64.415 kb on + strand, within GFF6751 at 64.415 kb on + strand, within GFF6751 at 64.416 kb on - strand, within GFF6751 at 64.933 kb on + strand at 64.934 kb on - strand at 64.934 kb on - strand at 64.934 kb on - strand at 64.934 kb on - strand at 64.934 kb on - strand at 65.110 kb on + strand, within GFF6752 at 65.110 kb on + strand, within GFF6752 at 65.176 kb on + strand, within GFF6752 at 65.176 kb on + strand, within GFF6752 at 65.239 kb on + strand, within GFF6752 at 65.239 kb on + strand, within GFF6752 at 65.239 kb on + strand, within GFF6752 at 65.240 kb on - strand, within GFF6752 at 65.240 kb on - strand, within GFF6752 at 65.240 kb on - strand, within GFF6752 at 65.246 kb on - strand, within GFF6752 at 65.258 kb on + strand, within GFF6752 at 65.258 kb on + strand, within GFF6752 at 65.452 kb on + strand, within GFF6752 at 65.453 kb on - strand, within GFF6752 at 65.864 kb on - strand, within GFF6752 at 66.118 kb on - strand, within GFF6752 at 66.382 kb on + strand, within GFF6752 at 66.697 kb on + strand, within GFF6752 at 66.724 kb on + strand, within GFF6752 at 66.724 kb on + strand, within GFF6752 at 66.724 kb on + strand, within GFF6752 at 66.725 kb on - strand, within GFF6752 at 66.725 kb on - strand, within GFF6752 at 66.725 kb on - strand, within GFF6752 at 66.725 kb on - strand, within GFF6752 at 66.806 kb on - strand at 66.922 kb on + strand at 66.923 kb on - strand at 66.923 kb on - strand at 66.923 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 63,990 + GFF6750 0.82 +1.0 64,035 + -0.8 64,035 + -0.6 64,035 + +1.0 64,035 + -0.1 64,035 + -2.0 64,035 + -0.7 64,035 + +0.1 64,035 + -0.0 64,035 + +0.3 64,035 + +0.0 64,035 + -0.7 64,035 + -0.2 64,036 - -0.3 64,036 - +1.7 64,036 - -0.8 64,036 - -0.3 64,036 - -0.1 64,036 - -0.0 64,103 + -1.1 64,104 - -0.5 64,121 + -0.6 64,121 + -1.0 64,121 + -0.4 64,173 - -1.3 64,173 - -0.8 64,173 - -0.9 64,173 - +0.7 64,415 + GFF6751 0.38 -0.3 64,415 + GFF6751 0.38 -0.4 64,416 - GFF6751 0.39 -0.3 64,933 + -1.0 64,934 - -1.3 64,934 - -1.1 64,934 - -0.6 64,934 - -0.6 64,934 - -0.4 65,110 + GFF6752 0.11 -1.0 65,110 + GFF6752 0.11 +2.3 65,176 + GFF6752 0.14 +1.2 65,176 + GFF6752 0.14 +0.0 65,239 + GFF6752 0.17 -0.1 65,239 + GFF6752 0.17 -0.3 65,239 + GFF6752 0.17 -0.4 65,240 - GFF6752 0.17 -0.5 65,240 - GFF6752 0.17 -1.4 65,240 - GFF6752 0.17 -0.5 65,246 - GFF6752 0.18 +0.7 65,258 + GFF6752 0.18 -0.6 65,258 + GFF6752 0.18 +0.7 65,452 + GFF6752 0.28 +0.9 65,453 - GFF6752 0.28 -3.1 65,864 - GFF6752 0.48 -0.1 66,118 - GFF6752 0.60 +1.2 66,382 + GFF6752 0.73 -0.8 66,697 + GFF6752 0.88 +1.1 66,724 + GFF6752 0.89 -2.3 66,724 + GFF6752 0.89 +0.9 66,724 + GFF6752 0.89 +0.3 66,725 - GFF6752 0.89 -1.2 66,725 - GFF6752 0.89 -0.4 66,725 - GFF6752 0.89 -1.2 66,725 - GFF6752 0.89 +0.6 66,806 - +0.0 66,922 + -0.9 66,923 - -0.5 66,923 - -0.3 66,923 - +0.6
Or see this region's nucleotide sequence