Strain Fitness in Variovorax sp. SCN45 around GFF6746

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6745 and GFF6746 overlap by 8 nucleotidesGFF6746 and GFF6747 overlap by 4 nucleotides GFF6745 - hypothetical protein, at 58,675 to 59,538 GFF6745 GFF6746 - dolichyl-phosphate-mannose-protein mannosyltransferase family protein, at 59,531 to 61,147 GFF6746 GFF6747 - Glycosyl transferase, family 2, at 61,144 to 62,229 GFF6747 Position (kb) 59 60 61 62Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 58.538 kb on + strandat 58.556 kb on + strandat 58.557 kb on - strandat 58.676 kb on + strandat 58.676 kb on + strandat 58.676 kb on + strandat 58.684 kb on + strandat 58.684 kb on + strandat 58.685 kb on - strandat 58.685 kb on - strandat 58.899 kb on - strand, within GFF6745at 58.925 kb on + strand, within GFF6745at 58.926 kb on - strand, within GFF6745at 58.926 kb on - strand, within GFF6745at 58.926 kb on - strand, within GFF6745at 59.057 kb on + strand, within GFF6745at 59.159 kb on + strand, within GFF6745at 59.160 kb on - strand, within GFF6745at 59.220 kb on - strand, within GFF6745at 59.220 kb on - strand, within GFF6745at 59.249 kb on + strand, within GFF6745at 59.250 kb on - strand, within GFF6745at 59.250 kb on - strand, within GFF6745at 59.771 kb on + strand, within GFF6746at 59.771 kb on + strand, within GFF6746at 59.771 kb on + strand, within GFF6746at 59.771 kb on + strand, within GFF6746at 59.772 kb on - strand, within GFF6746at 59.832 kb on + strand, within GFF6746at 59.832 kb on + strand, within GFF6746at 59.833 kb on - strand, within GFF6746at 59.833 kb on - strand, within GFF6746at 59.919 kb on + strand, within GFF6746at 59.935 kb on - strand, within GFF6746at 59.937 kb on + strand, within GFF6746at 59.938 kb on - strand, within GFF6746at 59.938 kb on - strand, within GFF6746at 59.938 kb on - strand, within GFF6746at 59.938 kb on - strand, within GFF6746at 59.938 kb on - strand, within GFF6746at 59.940 kb on + strand, within GFF6746at 59.940 kb on + strand, within GFF6746at 59.940 kb on + strand, within GFF6746at 59.940 kb on + strand, within GFF6746at 59.940 kb on + strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 59.941 kb on - strand, within GFF6746at 60.024 kb on + strand, within GFF6746at 60.342 kb on - strand, within GFF6746at 60.342 kb on - strand, within GFF6746at 60.342 kb on - strand, within GFF6746at 60.345 kb on + strand, within GFF6746at 60.345 kb on + strand, within GFF6746at 60.346 kb on - strand, within GFF6746at 60.346 kb on - strand, within GFF6746at 60.417 kb on + strand, within GFF6746at 60.417 kb on + strand, within GFF6746at 60.418 kb on - strand, within GFF6746at 60.418 kb on - strand, within GFF6746at 60.418 kb on - strand, within GFF6746at 60.436 kb on - strand, within GFF6746at 60.621 kb on + strand, within GFF6746at 60.622 kb on - strand, within GFF6746at 60.930 kb on + strand, within GFF6746at 60.930 kb on + strand, within GFF6746at 60.931 kb on - strand, within GFF6746at 61.199 kb on + strandat 61.199 kb on + strandat 61.199 kb on + strandat 61.226 kb on + strandat 61.241 kb on + strandat 61.241 kb on + strandat 61.241 kb on + strandat 61.241 kb on + strandat 61.242 kb on - strandat 61.358 kb on + strand, within GFF6747at 61.358 kb on + strand, within GFF6747at 61.359 kb on - strand, within GFF6747at 61.553 kb on + strand, within GFF6747at 61.554 kb on - strand, within GFF6747at 61.554 kb on - strand, within GFF6747at 61.554 kb on - strand, within GFF6747at 61.554 kb on - strand, within GFF6747at 61.554 kb on - strand, within GFF6747at 61.682 kb on + strand, within GFF6747at 61.682 kb on + strand, within GFF6747at 61.683 kb on - strand, within GFF6747at 61.683 kb on - strand, within GFF6747at 61.745 kb on + strand, within GFF6747at 61.745 kb on + strand, within GFF6747at 61.746 kb on - strand, within GFF6747at 61.746 kb on - strand, within GFF6747at 61.746 kb on - strand, within GFF6747at 62.063 kb on + strand, within GFF6747at 62.064 kb on - strand, within GFF6747

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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58,538 + -0.4
58,556 + -0.5
58,557 - +0.3
58,676 + -0.5
58,676 + +1.7
58,676 + -0.5
58,684 + -0.2
58,684 + -1.0
58,685 - -0.2
58,685 - +0.9
58,899 - GFF6745 0.26 +0.2
58,925 + GFF6745 0.29 +0.4
58,926 - GFF6745 0.29 -0.1
58,926 - GFF6745 0.29 -0.7
58,926 - GFF6745 0.29 -0.8
59,057 + GFF6745 0.44 -0.0
59,159 + GFF6745 0.56 -0.0
59,160 - GFF6745 0.56 +0.4
59,220 - GFF6745 0.63 -1.2
59,220 - GFF6745 0.63 +0.3
59,249 + GFF6745 0.66 -0.8
59,250 - GFF6745 0.67 +0.5
59,250 - GFF6745 0.67 -3.0
59,771 + GFF6746 0.15 -1.7
59,771 + GFF6746 0.15 +1.6
59,771 + GFF6746 0.15 +3.6
59,771 + GFF6746 0.15 -0.8
59,772 - GFF6746 0.15 +0.2
59,832 + GFF6746 0.19 +0.5
59,832 + GFF6746 0.19 +0.5
59,833 - GFF6746 0.19 +0.5
59,833 - GFF6746 0.19 +0.5
59,919 + GFF6746 0.24 -0.1
59,935 - GFF6746 0.25 +0.7
59,937 + GFF6746 0.25 +1.0
59,938 - GFF6746 0.25 +0.3
59,938 - GFF6746 0.25 +0.8
59,938 - GFF6746 0.25 -0.7
59,938 - GFF6746 0.25 -3.1
59,938 - GFF6746 0.25 -0.8
59,940 + GFF6746 0.25 +0.0
59,940 + GFF6746 0.25 +0.2
59,940 + GFF6746 0.25 +0.2
59,940 + GFF6746 0.25 -0.4
59,940 + GFF6746 0.25 -0.4
59,941 - GFF6746 0.25 +0.0
59,941 - GFF6746 0.25 +0.3
59,941 - GFF6746 0.25 -0.3
59,941 - GFF6746 0.25 -0.4
59,941 - GFF6746 0.25 +0.7
59,941 - GFF6746 0.25 +0.7
60,024 + GFF6746 0.30 -0.1
60,342 - GFF6746 0.50 +1.7
60,342 - GFF6746 0.50 +0.1
60,342 - GFF6746 0.50 +0.3
60,345 + GFF6746 0.50 +0.2
60,345 + GFF6746 0.50 -2.1
60,346 - GFF6746 0.50 +0.5
60,346 - GFF6746 0.50 -0.7
60,417 + GFF6746 0.55 +0.0
60,417 + GFF6746 0.55 -0.1
60,418 - GFF6746 0.55 +0.3
60,418 - GFF6746 0.55 +0.1
60,418 - GFF6746 0.55 +0.1
60,436 - GFF6746 0.56 +0.3
60,621 + GFF6746 0.67 +0.3
60,622 - GFF6746 0.67 +1.1
60,930 + GFF6746 0.87 +0.7
60,930 + GFF6746 0.87 +0.8
60,931 - GFF6746 0.87 -0.7
61,199 + -0.4
61,199 + -0.4
61,199 + -0.2
61,226 + -0.6
61,241 + +2.2
61,241 + +0.0
61,241 + -0.3
61,241 + -1.6
61,242 - -0.8
61,358 + GFF6747 0.20 -1.3
61,358 + GFF6747 0.20 +1.0
61,359 - GFF6747 0.20 -0.1
61,553 + GFF6747 0.38 +1.7
61,554 - GFF6747 0.38 -0.8
61,554 - GFF6747 0.38 +0.6
61,554 - GFF6747 0.38 -0.6
61,554 - GFF6747 0.38 +0.7
61,554 - GFF6747 0.38 -1.7
61,682 + GFF6747 0.50 -0.6
61,682 + GFF6747 0.50 -1.6
61,683 - GFF6747 0.50 +0.2
61,683 - GFF6747 0.50 +0.7
61,745 + GFF6747 0.55 -0.1
61,745 + GFF6747 0.55 +0.2
61,746 - GFF6747 0.55 +0.3
61,746 - GFF6747 0.55 +0.3
61,746 - GFF6747 0.55 -0.7
62,063 + GFF6747 0.85 -0.7
62,064 - GFF6747 0.85 -1.3

Or see this region's nucleotide sequence