Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6401 and GFF6402 overlap by 4 nucleotides GFF6402 and GFF6403 are separated by 27 nucleotides GFF6403 and GFF6404 are separated by 148 nucleotides
GFF6401 - Formiminoglutamic iminohydrolase (EC 3.5.3.13), at 75,354 to 76,736
GFF6401
GFF6402 - Imidazolonepropionase (EC 3.5.2.7), at 76,733 to 77,959
GFF6402
GFF6403 - Stresses-induced protein Ves (HutD), at 77,987 to 78,559
GFF6403
GFF6404 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 78,708 to 79,376
GFF6404
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 75.883 kb on + strand, within GFF6401 at 75.883 kb on + strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.890 kb on - strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.339 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.369 kb on + strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.370 kb on - strand, within GFF6401 at 76.417 kb on + strand, within GFF6401 at 76.418 kb on - strand, within GFF6401 at 76.474 kb on + strand, within GFF6401 at 76.474 kb on + strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.475 kb on - strand, within GFF6401 at 76.804 kb on - strand at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.091 kb on + strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.092 kb on - strand, within GFF6402 at 77.094 kb on + strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.095 kb on - strand, within GFF6402 at 77.194 kb on - strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.397 kb on + strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.398 kb on - strand, within GFF6402 at 77.472 kb on + strand, within GFF6402 at 77.561 kb on + strand, within GFF6402 at 77.561 kb on + strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.562 kb on - strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.685 kb on + strand, within GFF6402 at 77.686 kb on - strand, within GFF6402 at 77.736 kb on + strand, within GFF6402 at 77.737 kb on - strand, within GFF6402 at 77.737 kb on - strand, within GFF6402 at 78.299 kb on + strand, within GFF6403 at 78.299 kb on + strand, within GFF6403 at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.694 kb on + strand at 78.695 kb on - strand at 78.695 kb on - strand at 78.695 kb on - strand at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.787 kb on + strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.788 kb on - strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.808 kb on + strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.809 kb on - strand, within GFF6404 at 78.927 kb on + strand, within GFF6404
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 75,883 + GFF6401 0.38 +0.3 75,883 + GFF6401 0.38 +0.1 75,884 - GFF6401 0.38 +3.2 75,884 - GFF6401 0.38 -0.7 75,890 - GFF6401 0.39 +0.2 76,255 + GFF6401 0.65 +0.2 76,255 + GFF6401 0.65 -1.0 76,255 + GFF6401 0.65 -0.9 76,339 + GFF6401 0.71 -0.7 76,369 + GFF6401 0.73 +0.1 76,369 + GFF6401 0.73 -0.6 76,369 + GFF6401 0.73 +0.0 76,369 + GFF6401 0.73 +0.9 76,369 + GFF6401 0.73 +0.3 76,370 - GFF6401 0.73 +0.2 76,370 - GFF6401 0.73 -0.9 76,370 - GFF6401 0.73 -1.7 76,370 - GFF6401 0.73 +0.7 76,417 + GFF6401 0.77 -1.0 76,418 - GFF6401 0.77 -0.8 76,474 + GFF6401 0.81 -0.5 76,474 + GFF6401 0.81 +0.2 76,475 - GFF6401 0.81 -0.0 76,475 - GFF6401 0.81 +1.0 76,475 - GFF6401 0.81 +0.3 76,475 - GFF6401 0.81 +1.5 76,475 - GFF6401 0.81 +0.1 76,475 - GFF6401 0.81 -0.4 76,804 - +0.4 77,091 + GFF6402 0.29 -0.4 77,091 + GFF6402 0.29 +0.5 77,091 + GFF6402 0.29 +0.1 77,091 + GFF6402 0.29 -0.3 77,091 + GFF6402 0.29 +0.1 77,091 + GFF6402 0.29 +1.7 77,091 + GFF6402 0.29 -0.4 77,092 - GFF6402 0.29 +1.0 77,092 - GFF6402 0.29 -0.4 77,092 - GFF6402 0.29 -0.5 77,092 - GFF6402 0.29 -0.4 77,092 - GFF6402 0.29 +0.7 77,092 - GFF6402 0.29 +1.2 77,092 - GFF6402 0.29 +0.4 77,092 - GFF6402 0.29 +1.0 77,092 - GFF6402 0.29 -1.0 77,094 + GFF6402 0.29 -0.2 77,095 - GFF6402 0.30 +0.7 77,095 - GFF6402 0.30 -0.7 77,095 - GFF6402 0.30 -0.2 77,194 - GFF6402 0.38 -0.5 77,397 + GFF6402 0.54 -0.4 77,397 + GFF6402 0.54 -0.6 77,397 + GFF6402 0.54 -0.4 77,398 - GFF6402 0.54 -0.2 77,398 - GFF6402 0.54 -0.3 77,398 - GFF6402 0.54 -0.1 77,398 - GFF6402 0.54 -0.3 77,398 - GFF6402 0.54 -0.7 77,398 - GFF6402 0.54 +0.3 77,398 - GFF6402 0.54 -0.0 77,398 - GFF6402 0.54 +0.4 77,472 + GFF6402 0.60 -1.0 77,561 + GFF6402 0.67 +0.1 77,561 + GFF6402 0.67 -0.8 77,562 - GFF6402 0.68 +0.9 77,562 - GFF6402 0.68 -0.8 77,562 - GFF6402 0.68 +1.6 77,562 - GFF6402 0.68 +1.0 77,562 - GFF6402 0.68 -1.3 77,562 - GFF6402 0.68 -0.7 77,562 - GFF6402 0.68 +0.4 77,562 - GFF6402 0.68 +0.1 77,685 + GFF6402 0.78 -0.4 77,685 + GFF6402 0.78 -0.2 77,685 + GFF6402 0.78 -0.7 77,686 - GFF6402 0.78 +0.1 77,736 + GFF6402 0.82 +0.3 77,737 - GFF6402 0.82 -0.2 77,737 - GFF6402 0.82 -2.0 78,299 + GFF6403 0.54 -0.5 78,299 + GFF6403 0.54 -1.0 78,694 + -0.8 78,694 + -1.0 78,694 + +1.0 78,694 + -0.2 78,694 + -1.9 78,694 + +0.2 78,694 + -1.2 78,695 - +0.3 78,695 - -0.1 78,695 - -0.2 78,787 + GFF6404 0.12 -0.3 78,787 + GFF6404 0.12 -0.0 78,787 + GFF6404 0.12 +1.0 78,787 + GFF6404 0.12 +0.2 78,787 + GFF6404 0.12 +0.4 78,788 - GFF6404 0.12 +0.1 78,788 - GFF6404 0.12 -0.1 78,788 - GFF6404 0.12 -0.9 78,788 - GFF6404 0.12 -0.0 78,808 + GFF6404 0.15 -0.4 78,808 + GFF6404 0.15 -1.0 78,808 + GFF6404 0.15 +0.4 78,809 - GFF6404 0.15 +0.3 78,809 - GFF6404 0.15 +1.5 78,809 - GFF6404 0.15 +0.2 78,809 - GFF6404 0.15 -0.0 78,809 - GFF6404 0.15 +0.7 78,927 + GFF6404 0.33 -0.5
Or see this region's nucleotide sequence