Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF62 and GFF63 are separated by 7 nucleotides GFF63 and GFF64 overlap by 4 nucleotides GFF64 and GFF65 are separated by 35 nucleotides
GFF62 - hypothetical protein, at 78,076 to 79,422
GFF62
GFF63 - Putative glycosyltransferase, at 79,430 to 80,512
GFF63
GFF64 - hypothetical protein, at 80,509 to 81,462
GFF64
GFF65 - hypothetical protein, at 81,498 to 82,433
GFF65
Position (kb) 79
80
81 Strain fitness (log2 ratio) -2
-1
0
1 at 79.114 kb on + strand, within GFF62 at 79.114 kb on + strand, within GFF62 at 79.252 kb on + strand, within GFF62 at 79.253 kb on - strand, within GFF62 at 79.439 kb on + strand at 79.439 kb on + strand at 79.439 kb on + strand at 79.440 kb on - strand at 79.440 kb on - strand at 79.440 kb on - strand at 79.448 kb on + strand at 79.449 kb on - strand at 79.449 kb on - strand at 79.574 kb on + strand, within GFF63 at 79.574 kb on + strand, within GFF63 at 79.575 kb on - strand, within GFF63 at 79.575 kb on - strand, within GFF63 at 79.628 kb on + strand, within GFF63 at 79.628 kb on + strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.637 kb on + strand, within GFF63 at 79.673 kb on + strand, within GFF63 at 79.673 kb on + strand, within GFF63 at 79.691 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.766 kb on + strand, within GFF63 at 79.776 kb on - strand, within GFF63 at 80.000 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.007 kb on - strand, within GFF63 at 80.007 kb on - strand, within GFF63 at 80.202 kb on - strand, within GFF63 at 80.202 kb on - strand, within GFF63 at 80.369 kb on + strand, within GFF63 at 80.369 kb on + strand, within GFF63 at 80.370 kb on - strand, within GFF63 at 80.384 kb on + strand, within GFF63 at 80.462 kb on + strand at 80.463 kb on - strand at 80.463 kb on - strand at 80.642 kb on - strand, within GFF64 at 80.642 kb on - strand, within GFF64 at 80.642 kb on - strand, within GFF64 at 80.765 kb on - strand, within GFF64 at 80.765 kb on - strand, within GFF64 at 80.887 kb on + strand, within GFF64 at 80.888 kb on - strand, within GFF64 at 80.888 kb on - strand, within GFF64 at 81.058 kb on + strand, within GFF64 at 81.058 kb on + strand, within GFF64 at 81.058 kb on + strand, within GFF64 at 81.058 kb on + strand, within GFF64 at 81.058 kb on + strand, within GFF64 at 81.059 kb on - strand, within GFF64 at 81.059 kb on - strand, within GFF64 at 81.214 kb on + strand, within GFF64 at 81.214 kb on + strand, within GFF64 at 81.214 kb on + strand, within GFF64 at 81.215 kb on - strand, within GFF64 at 81.215 kb on - strand, within GFF64 at 81.215 kb on - strand, within GFF64 at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.477 kb on + strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.478 kb on - strand at 81.481 kb on + strand at 81.482 kb on - strand at 81.482 kb on - strand at 81.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 79,114 + GFF62 0.77 +0.5 79,114 + GFF62 0.77 -1.7 79,252 + GFF62 0.87 +1.1 79,253 - GFF62 0.87 -0.9 79,439 + -1.0 79,439 + +1.1 79,439 + +0.1 79,440 - -0.7 79,440 - -0.0 79,440 - +1.5 79,448 + +0.1 79,449 - +0.0 79,449 - -0.9 79,574 + GFF63 0.13 +0.1 79,574 + GFF63 0.13 -2.0 79,575 - GFF63 0.13 -0.3 79,575 - GFF63 0.13 -0.8 79,628 + GFF63 0.18 -0.0 79,628 + GFF63 0.18 -0.7 79,629 - GFF63 0.18 -0.3 79,629 - GFF63 0.18 -1.6 79,629 - GFF63 0.18 -0.2 79,637 + GFF63 0.19 -0.1 79,673 + GFF63 0.22 +0.5 79,673 + GFF63 0.22 -0.8 79,691 + GFF63 0.24 -1.4 79,757 + GFF63 0.30 +0.6 79,757 + GFF63 0.30 -0.3 79,757 + GFF63 0.30 -2.0 79,758 - GFF63 0.30 -1.7 79,758 - GFF63 0.30 +0.3 79,758 - GFF63 0.30 +0.1 79,758 - GFF63 0.30 -0.2 79,758 - GFF63 0.30 -1.1 79,758 - GFF63 0.30 -0.1 79,766 + GFF63 0.31 -0.2 79,776 - GFF63 0.32 -0.9 80,000 + GFF63 0.53 +1.5 80,006 + GFF63 0.53 -0.5 80,006 + GFF63 0.53 -0.5 80,006 + GFF63 0.53 -0.5 80,007 - GFF63 0.53 +0.4 80,007 - GFF63 0.53 +0.2 80,202 - GFF63 0.71 -0.2 80,202 - GFF63 0.71 +0.3 80,369 + GFF63 0.87 +0.1 80,369 + GFF63 0.87 -0.1 80,370 - GFF63 0.87 +0.3 80,384 + GFF63 0.88 +0.5 80,462 + +1.0 80,463 - -0.4 80,463 - +0.9 80,642 - GFF64 0.14 +0.2 80,642 - GFF64 0.14 -1.0 80,642 - GFF64 0.14 -1.3 80,765 - GFF64 0.27 -0.9 80,765 - GFF64 0.27 -0.6 80,887 + GFF64 0.40 +1.3 80,888 - GFF64 0.40 -0.9 80,888 - GFF64 0.40 -1.0 81,058 + GFF64 0.58 -0.9 81,058 + GFF64 0.58 -1.1 81,058 + GFF64 0.58 -0.5 81,058 + GFF64 0.58 -0.4 81,058 + GFF64 0.58 -0.9 81,059 - GFF64 0.58 -0.2 81,059 - GFF64 0.58 -1.3 81,214 + GFF64 0.74 -0.7 81,214 + GFF64 0.74 +0.6 81,214 + GFF64 0.74 -0.3 81,215 - GFF64 0.74 +0.9 81,215 - GFF64 0.74 +0.4 81,215 - GFF64 0.74 -0.1 81,477 + +0.2 81,477 + -0.0 81,477 + -0.9 81,477 + -0.4 81,477 + -0.1 81,477 + -0.8 81,477 + +1.6 81,477 + -0.8 81,478 - -0.1 81,478 - -0.4 81,478 - -0.3 81,478 - +0.1 81,478 - +0.9 81,481 + -0.7 81,482 - -0.2 81,482 - -0.3 81,506 + -0.0
Or see this region's nucleotide sequence