Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6073 and GFF6074 are separated by 19 nucleotides GFF6074 and GFF6075 are separated by 145 nucleotides
GFF6073 - Tripartite tricarboxylate transporter TctA family, at 135,256 to 136,767
GFF6073
GFF6074 - Tripartite tricarboxylate transporter TctB family, at 136,787 to 137,275
GFF6074
GFF6075 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 137,421 to 139,217
GFF6075
Position (kb) 136
137
138 Strain fitness (log2 ratio) -2
-1
0
1
2 at 135.929 kb on + strand, within GFF6073 at 135.929 kb on + strand, within GFF6073 at 135.929 kb on + strand, within GFF6073 at 135.930 kb on - strand, within GFF6073 at 135.930 kb on - strand, within GFF6073 at 135.930 kb on - strand, within GFF6073 at 136.611 kb on - strand, within GFF6073 at 136.704 kb on - strand at 136.704 kb on - strand at 136.704 kb on - strand at 136.851 kb on + strand, within GFF6074 at 136.851 kb on + strand, within GFF6074 at 136.851 kb on + strand, within GFF6074 at 136.852 kb on - strand, within GFF6074 at 136.852 kb on - strand, within GFF6074 at 136.852 kb on - strand, within GFF6074 at 136.852 kb on - strand, within GFF6074 at 136.941 kb on + strand, within GFF6074 at 136.941 kb on + strand, within GFF6074 at 136.941 kb on + strand, within GFF6074 at 136.942 kb on - strand, within GFF6074 at 136.942 kb on - strand, within GFF6074 at 137.154 kb on + strand, within GFF6074 at 137.154 kb on + strand, within GFF6074 at 137.155 kb on - strand, within GFF6074 at 137.190 kb on + strand, within GFF6074 at 137.190 kb on + strand, within GFF6074 at 137.191 kb on - strand, within GFF6074 at 137.191 kb on - strand, within GFF6074 at 137.191 kb on - strand, within GFF6074 at 137.191 kb on - strand, within GFF6074 at 137.191 kb on - strand, within GFF6074 at 137.363 kb on - strand at 137.415 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.417 kb on + strand at 137.418 kb on - strand at 137.418 kb on - strand at 137.418 kb on - strand at 137.418 kb on - strand at 137.423 kb on - strand at 137.605 kb on + strand, within GFF6075 at 137.605 kb on + strand, within GFF6075 at 137.605 kb on + strand, within GFF6075 at 137.606 kb on - strand, within GFF6075 at 137.626 kb on + strand, within GFF6075 at 137.626 kb on + strand, within GFF6075 at 137.627 kb on - strand, within GFF6075 at 137.665 kb on + strand, within GFF6075 at 137.815 kb on + strand, within GFF6075 at 137.816 kb on - strand, within GFF6075 at 137.816 kb on - strand, within GFF6075 at 138.125 kb on - strand, within GFF6075 at 138.125 kb on - strand, within GFF6075
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 135,929 + GFF6073 0.45 +0.1 135,929 + GFF6073 0.45 +0.7 135,929 + GFF6073 0.45 +0.0 135,930 - GFF6073 0.45 -0.1 135,930 - GFF6073 0.45 +1.7 135,930 - GFF6073 0.45 +0.3 136,611 - GFF6073 0.90 -1.4 136,704 - -1.0 136,704 - +0.9 136,704 - -0.7 136,851 + GFF6074 0.13 +1.5 136,851 + GFF6074 0.13 -0.7 136,851 + GFF6074 0.13 +0.8 136,852 - GFF6074 0.13 -0.3 136,852 - GFF6074 0.13 -0.5 136,852 - GFF6074 0.13 -0.8 136,852 - GFF6074 0.13 -0.9 136,941 + GFF6074 0.31 -0.1 136,941 + GFF6074 0.31 +0.8 136,941 + GFF6074 0.31 +0.1 136,942 - GFF6074 0.32 -0.4 136,942 - GFF6074 0.32 -0.9 137,154 + GFF6074 0.75 -0.5 137,154 + GFF6074 0.75 -1.8 137,155 - GFF6074 0.75 +0.7 137,190 + GFF6074 0.82 -0.4 137,190 + GFF6074 0.82 -1.1 137,191 - GFF6074 0.83 +0.6 137,191 - GFF6074 0.83 +1.5 137,191 - GFF6074 0.83 -0.7 137,191 - GFF6074 0.83 +1.2 137,191 - GFF6074 0.83 +0.1 137,363 - -0.5 137,415 + -0.2 137,417 + -0.0 137,417 + +0.2 137,417 + -0.9 137,417 + -0.3 137,417 + -1.7 137,417 + -1.5 137,417 + -2.5 137,417 + -0.2 137,417 + +0.1 137,418 - +0.1 137,418 - -0.7 137,418 - +2.7 137,418 - +1.1 137,423 - -0.2 137,605 + GFF6075 0.10 +0.2 137,605 + GFF6075 0.10 -1.6 137,605 + GFF6075 0.10 -0.3 137,606 - GFF6075 0.10 -0.7 137,626 + GFF6075 0.11 -1.1 137,626 + GFF6075 0.11 -0.5 137,627 - GFF6075 0.11 -1.1 137,665 + GFF6075 0.14 +0.7 137,815 + GFF6075 0.22 -0.6 137,816 - GFF6075 0.22 -0.9 137,816 - GFF6075 0.22 -0.5 138,125 - GFF6075 0.39 -0.5 138,125 - GFF6075 0.39 -0.7
Or see this region's nucleotide sequence