Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6007 and GFF6008 are separated by 193 nucleotides GFF6008 and GFF6009 are separated by 13 nucleotides
GFF6007 - Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8), at 65,384 to 67,624
GFF6007
GFF6008 - Citrate synthase (si) (EC 2.3.3.1), at 67,818 to 68,603
GFF6008
GFF6009 - Acyl-CoA dehydrogenase STM0857, at 68,617 to 69,780
GFF6009
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 66.877 kb on + strand, within GFF6007 at 66.877 kb on + strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.948 kb on + strand, within GFF6007 at 67.666 kb on - strand at 68.061 kb on + strand, within GFF6008 at 68.062 kb on - strand, within GFF6008 at 68.062 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008 at 68.641 kb on + strand at 68.642 kb on - strand at 68.642 kb on - strand at 68.695 kb on + strand at 68.695 kb on + strand at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.887 kb on + strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.888 kb on - strand, within GFF6009 at 68.938 kb on + strand, within GFF6009 at 69.055 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.325 kb on + strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.326 kb on - strand, within GFF6009 at 69.416 kb on - strand, within GFF6009 at 69.524 kb on - strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.586 kb on + strand, within GFF6009 at 69.587 kb on - strand, within GFF6009
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 66,877 + GFF6007 0.67 -0.8 66,877 + GFF6007 0.67 -0.9 66,878 - GFF6007 0.67 +0.8 66,878 - GFF6007 0.67 +0.4 66,878 - GFF6007 0.67 -0.3 66,948 + GFF6007 0.70 -0.1 67,666 - +0.1 68,061 + GFF6008 0.31 +0.7 68,062 - GFF6008 0.31 +0.2 68,062 - GFF6008 0.31 -1.3 68,350 - GFF6008 0.68 -1.1 68,350 - GFF6008 0.68 +0.3 68,350 - GFF6008 0.68 -3.0 68,641 + -0.0 68,642 - -0.1 68,642 - -0.5 68,695 + -1.0 68,695 + +0.6 68,887 + GFF6009 0.23 -0.7 68,887 + GFF6009 0.23 +0.3 68,887 + GFF6009 0.23 -0.6 68,887 + GFF6009 0.23 -0.0 68,888 - GFF6009 0.23 -0.4 68,888 - GFF6009 0.23 -0.2 68,888 - GFF6009 0.23 -1.2 68,938 + GFF6009 0.28 -0.3 69,055 + GFF6009 0.38 +0.6 69,325 + GFF6009 0.61 +0.3 69,325 + GFF6009 0.61 -1.1 69,325 + GFF6009 0.61 +1.3 69,326 - GFF6009 0.61 -0.2 69,326 - GFF6009 0.61 +0.1 69,416 - GFF6009 0.69 -1.3 69,524 - GFF6009 0.78 -0.3 69,586 + GFF6009 0.83 +0.5 69,586 + GFF6009 0.83 -0.1 69,587 - GFF6009 0.83 -0.3
Or see this region's nucleotide sequence