Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5928 and GFF5929 are separated by 25 nucleotides GFF5929 and GFF5930 are separated by 21 nucleotides GFF5930 and GFF5931 are separated by 15 nucleotides GFF5931 and GFF5932 are separated by 13 nucleotides
GFF5928 - hypothetical protein, at 143,234 to 144,346
GFF5928
GFF5929 - Mll2356 protein, at 144,372 to 144,854
GFF5929
GFF5930 - VgrG protein, at 144,876 to 147,254
GFF5930
GFF5931 - FIG01219766: hypothetical protein, at 147,270 to 147,665
GFF5931
GFF5932 - no description, at 147,679 to 148,311
GFF5932
Position (kb)
144
145
146
147
148 Strain fitness (log2 ratio)
-1
0
1
2 at 143.926 kb on - strand, within GFF5928 at 144.022 kb on - strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.433 kb on + strand, within GFF5929 at 144.433 kb on + strand, within GFF5929 at 144.434 kb on - strand, within GFF5929 at 144.860 kb on + strand at 144.860 kb on + strand at 144.861 kb on - strand at 145.310 kb on + strand, within GFF5930 at 145.311 kb on - strand, within GFF5930 at 145.627 kb on + strand, within GFF5930 at 145.627 kb on + strand, within GFF5930 at 145.759 kb on + strand, within GFF5930 at 145.844 kb on - strand, within GFF5930 at 145.969 kb on - strand, within GFF5930 at 145.969 kb on - strand, within GFF5930 at 146.557 kb on + strand, within GFF5930 at 146.558 kb on - strand, within GFF5930 at 146.558 kb on - strand, within GFF5930 at 146.737 kb on + strand, within GFF5930 at 146.737 kb on + strand, within GFF5930 at 146.738 kb on - strand, within GFF5930 at 146.738 kb on - strand, within GFF5930 at 146.986 kb on + strand, within GFF5930
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 143,926 - GFF5928 0.62 -0.2 144,022 - GFF5928 0.71 -0.2 144,132 + GFF5928 0.81 -0.5 144,132 + GFF5928 0.81 -0.8 144,132 + GFF5928 0.81 -0.3 144,132 + GFF5928 0.81 -1.0 144,133 - GFF5928 0.81 -0.2 144,133 - GFF5928 0.81 +0.1 144,133 - GFF5928 0.81 +0.1 144,433 + GFF5929 0.13 +0.6 144,433 + GFF5929 0.13 +0.0 144,434 - GFF5929 0.13 -0.2 144,860 + -0.2 144,860 + -1.5 144,861 - -0.3 145,310 + GFF5930 0.18 +0.2 145,311 - GFF5930 0.18 -1.1 145,627 + GFF5930 0.32 +0.1 145,627 + GFF5930 0.32 +0.0 145,759 + GFF5930 0.37 +0.1 145,844 - GFF5930 0.41 +2.1 145,969 - GFF5930 0.46 -0.6 145,969 - GFF5930 0.46 -0.9 146,557 + GFF5930 0.71 +0.4 146,558 - GFF5930 0.71 +0.6 146,558 - GFF5930 0.71 +0.4 146,737 + GFF5930 0.78 -0.2 146,737 + GFF5930 0.78 -0.3 146,738 - GFF5930 0.78 -0.2 146,738 - GFF5930 0.78 +0.7 146,986 + GFF5930 0.89 -0.7
Or see this region's nucleotide sequence