Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5767 and GFF5768 are separated by 28 nucleotides GFF5768 and GFF5769 are separated by 1 nucleotides GFF5769 and GFF5770 are separated by 147 nucleotides
GFF5767 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 139,610 to 140,695
GFF5767
GFF5768 - Chemotaxis regulator - transmits chemoreceptor signals to flagellar motor components CheY, at 140,724 to 141,122
GFF5768
GFF5769 - Chemotaxis response - phosphatase CheZ, at 141,124 to 141,768
GFF5769
GFF5770 - Flagellar biosynthesis protein FlhB, at 141,916 to 143,070
GFF5770
Position (kb)
141
142 Strain fitness (log2 ratio)
-1
0
1
2 at 140.309 kb on + strand, within GFF5767 at 140.315 kb on + strand, within GFF5767 at 140.316 kb on - strand, within GFF5767 at 140.316 kb on - strand, within GFF5767 at 140.316 kb on - strand, within GFF5767 at 140.316 kb on - strand, within GFF5767 at 140.366 kb on + strand, within GFF5767 at 140.366 kb on + strand, within GFF5767 at 140.367 kb on - strand, within GFF5767 at 140.448 kb on - strand, within GFF5767 at 140.650 kb on - strand at 140.720 kb on + strand at 141.298 kb on + strand, within GFF5769 at 141.299 kb on - strand, within GFF5769 at 141.479 kb on - strand, within GFF5769 at 141.766 kb on + strand at 141.833 kb on - strand at 141.887 kb on + strand at 141.888 kb on - strand at 141.888 kb on - strand at 141.888 kb on - strand at 142.456 kb on + strand, within GFF5770 at 142.456 kb on + strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.457 kb on - strand, within GFF5770 at 142.561 kb on + strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.562 kb on - strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.750 kb on + strand, within GFF5770 at 142.751 kb on - strand, within GFF5770
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 140,309 + GFF5767 0.64 +0.5 140,315 + GFF5767 0.65 -1.5 140,316 - GFF5767 0.65 -0.3 140,316 - GFF5767 0.65 +0.7 140,316 - GFF5767 0.65 +0.1 140,316 - GFF5767 0.65 -0.5 140,366 + GFF5767 0.70 -1.7 140,366 + GFF5767 0.70 -0.0 140,367 - GFF5767 0.70 -0.1 140,448 - GFF5767 0.77 -0.6 140,650 - +0.5 140,720 + -1.3 141,298 + GFF5769 0.27 +0.5 141,299 - GFF5769 0.27 -0.2 141,479 - GFF5769 0.55 +0.9 141,766 + +0.1 141,833 - -1.3 141,887 + -0.5 141,888 - -0.1 141,888 - +1.3 141,888 - -0.7 142,456 + GFF5770 0.47 -0.6 142,456 + GFF5770 0.47 -1.5 142,457 - GFF5770 0.47 +2.0 142,457 - GFF5770 0.47 +0.1 142,561 + GFF5770 0.56 -0.4 142,562 - GFF5770 0.56 +0.1 142,562 - GFF5770 0.56 -1.1 142,750 + GFF5770 0.72 -0.7 142,750 + GFF5770 0.72 +0.6 142,751 - GFF5770 0.72 -0.3
Or see this region's nucleotide sequence